DOI: 10.1101/gad.824700. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription

Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription

10.1101/gad.824700

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The activities of several mRNA processing factors are coupled to transcription through binding to RNA polymerase II (Pol II). The largest subunit of Pol II contains a repetitive carboxy-terminal domain (CTD) that becomes highly phosphorylated during transcription. mRNA-capping enzyme binds only to phosphorylated CTD, whereas other processing factors may bind to both phosphorylated and unphosphorylated forms. Capping occurs soon after transcription initiation and before other processing events, raising the question of whether capping components remain associated with the transcription complex after they have modified the 5′ end of the mRNA. Chromatin immunoprecipitation in Saccharomyces cerevisiae shows that capping enzyme cross-links to promoters but not coding regions. In contrast, the mRNA cap methyltransferase and the Hrp1/CFIB polyadenylation factor cross-link to both promoter and coding regions. Remarkably, the phosphorylation pattern of the CTD changes during transcription. Ser 5 phosphorylation is detected primarily at promoter regions dependent on TFIIH. In contrast, Ser 2 phosphorylation is seen only in coding regions. These results suggest a dynamic association of mRNA processing factors with differently modified forms of the polymerase throughout the transcription cycle.

Bibliography

Komarnitsky, P., Cho, E.-J., & Buratowski, S. (2000). Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription. Genes & Development, 14(19), 2452â2460.

Authors 3

Philip Komarnitsky (first)
Eun-Jung Cho (additional)
Stephen Buratowski (additional)

References 38 Referenced 878

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Dates

Type	When
Created	23 years ago (July 26, 2002, 7:59 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 10:36 p.m.)
Indexed	1 day, 5 hours ago (Aug. 20, 2025, 9:07 a.m.)
Issued	24 years, 10 months ago (Oct. 1, 2000)
Published	24 years, 10 months ago (Oct. 1, 2000)
Published Online	24 years, 10 months ago (Oct. 1, 2000)
Published Print	24 years, 10 months ago (Oct. 1, 2000)

Funders 0

None

BibTeX

@article{Komarnitsky_2000, title={Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription}, volume={14}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.824700}, DOI={10.1101/gad.824700}, number={19}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Komarnitsky, Philip and Cho, Eun-Jung and Buratowski, Stephen}, year={2000}, month=oct, pages={2452–2460} }

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