DOI: 10.1101/gad.8.24.2974. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth.

Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth.

10.1101/gad.8.24.2974

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Diploid Saccharomyces cerevisiae strains starved for nitrogen undergo a developmental transition from a colonial form of growth to a filamentous pseudohyphal form. This dimorphism requires a polar budding pattern and elements of the MAP kinase signal transduction pathway essential for mating pheromone response in haploids. We report here that haploid strains exhibit an invasive growth behavior with many similarities to pseudohyphal development, including filament formation and agar penetration. Haploid filament formation depends on a switch from an axial to a bipolar mode of bud site selection. Filament formation is distinct from agar penetration in both haploids and diploids. We find that the same components of the MAP kinase cascade necessary for diploid pseudohyphal development (STE20, STE11, STE7, and STE12) are also required for both filament formation and agar penetration in haploids. Thus, haploid yeast cells can enter either of two developmental pathways: mating or invasive growth, both of which depend on elements of a single MAP kinase cascade. Our results provide a novel developmental model to study the dynamics of signal transduction, with implications for higher eukaryotes.

Bibliography

Roberts, R. L., & Fink, G. R. (1994). Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes & Development, 8(24), 2974â2985.

Authors 2

R L Roberts (first)
G R Fink (additional)

References 47 Referenced 514

10.1101/gad.8.12.1367
10.1073/pnas.86.24.9976
10.1016/0968-0004(94)90147-3
10.1016/S0021-9258(17)42228-9 / J. Biol. Chem. / Endothelin-1 and fibroblast growth factors stimulate the mitogen-activated protein kinase signaling cascade in cardiac myocytes. The potential role of the cascade in the integration of two signaling pathways leading to myocyte hypertrophy. (1994)
10.1016/0092-8674(92)90182-C
10.1126/science.7681220
10.1016/0092-8674(94)90362-X
10.1101/gad.6.7.1305
10.1016/0092-8674(90)90503-7
10.1016/0092-8674(91)90015-Q
10.1016/0092-8674(94)90427-8
10.1007/BF00334769
10.1126/science.1411546
10.1016/0092-8674(87)90166-8
10.1101/gad.4.4.492
10.1101/SQB.1991.056.01.007
10.1073/pnas.88.21.9392
10.1091/mbc.4.5.495
10.1016/0898-6568(93)90078-Z
10.1128/jb.80.4.567-568.1960 / J. Bacteriology / Bud position in Saccharomyces cerevisiae. (1960)
10.1101/gad.6.7.1280
10.1126/science.1496375
{'key': '2021111418193754000_8.24.2974.23', 'first-page': '2100', 'article-title': 'Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development.', 'volume': '14', 'year': '1994', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. (1994)
10.1016/0092-8674(92)90079-R
10.1016/0304-4165(66)90387-4 / Biochim. Biophys. Acta / Multiplicity of the amino acid permeases in S. cerevisiae. (1966)
{'key': '2021111418193754000_8.24.2974.26', 'first-page': '2952', 'article-title': 'Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae.', 'volume': '11', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. (1991)
10.1101/gad.8.3.313
10.1091/mbc.5.9.1003
10.1038/358417a0
10.1101/gad.8.2.160
10.1126/science.8385802
10.1002/j.1460-2075.1992.tb05587.x / EMBO J. / The protein kinase homologue Ste20p is required to link the yeast pheromone response G protein βγ subunits to downstream signaling components. (1992)
10.1126/science.8259520
Liu, H., Koehler, J. and Fink. G.R. 1994. Suppression of hyphal formation in Candida albicans by mutation of a STE12 homolog. Science (in press).
10.1073/pnas.91.16.7762
10.1016/0968-0004(92)90003-R
10.1073/pnas.90.2.452
10.1101/gad.4.11.1862
10.1016/0896-6273(92)90177-F
Sherman, F., G.R. Fink, and J. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
10.1101/gad.6.7.1293
10.1042/bj2880351
{'key': '2021111418193754000_8.24.2974.43', 'first-page': '2316', 'article-title': 'Two genes required for cell fusion during yeast conjugation: Evidence for a pheromone-induced surface protein.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Two genes required for cell fusion during yeast conjugation: Evidence for a pheromone-induced surface protein. (1987)
{'key': '2021111418193754000_8.24.2974.44', 'first-page': '749', 'article-title': 'The yeast repeated element sigma contains a hormone-inducible promoter.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell. Bio.'} / Mol. Cell. Bio. / The yeast repeated element sigma contains a hormone-inducible promoter. (1987)
10.1016/0092-8674(89)90249-3
10.1101/gad.8.2.147
{'key': '2021111418193754000_8.24.2974.47', 'first-page': '2069', 'article-title': 'Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases.', 'volume': '13', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases. (1993)

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:28 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 6, 2025, 8:56 a.m.)
Issued	30 years, 8 months ago (Dec. 15, 1994)
Published	30 years, 8 months ago (Dec. 15, 1994)
Published Online	30 years, 8 months ago (Dec. 15, 1994)
Published Print	30 years, 8 months ago (Dec. 15, 1994)

Funders 0

None

BibTeX

@article{Roberts_1994, title={Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth.}, volume={8}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.8.24.2974}, DOI={10.1101/gad.8.24.2974}, number={24}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Roberts, R L and Fink, G R}, year={1994}, month=dec, pages={2974–2985} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T12:56:46Z",
    "timestamp": 1754485006073
  },
  "reference-count": 47,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "24",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1994,
        12,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Diploid Saccharomyces cerevisiae strains starved for nitrogen undergo a developmental transition from a colonial form of growth to a filamentous pseudohyphal form. This dimorphism requires a polar budding pattern and elements of the MAP kinase signal transduction pathway essential for mating pheromone response in haploids. We report here that haploid strains exhibit an invasive growth behavior with many similarities to pseudohyphal development, including filament formation and agar penetration. Haploid filament formation depends on a switch from an axial to a bipolar mode of bud site selection. Filament formation is distinct from agar penetration in both haploids and diploids. We find that the same components of the MAP kinase cascade necessary for diploid pseudohyphal development (STE20, STE11, STE7, and STE12) are also required for both filament formation and agar penetration in haploids. Thus, haploid yeast cells can enter either of two developmental pathways: mating or invasive growth, both of which depend on elements of a single MAP kinase cascade. Our results provide a novel developmental model to study the dynamics of signal transduction, with implications for higher eukaryotes.</jats:p>",
  "DOI": "10.1101/gad.8.24.2974",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        6,
        5
      ]
    ],
    "date-time": "2007-06-05T21:15:46Z",
    "timestamp": 1181078146000
  },
  "page": "2974-2985",
  "source": "Crossref",
  "is-referenced-by-count": 514,
  "title": "Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth.",
  "prefix": "10.1101",
  "volume": "8",
  "author": [
    {
      "given": "R L",
      "family": "Roberts",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "G R",
      "family": "Fink",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        1994,
        12,
        15
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111418193754000_8.24.2974.1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.12.1367"
    },
    {
      "key": "2021111418193754000_8.24.2974.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.86.24.9976"
    },
    {
      "key": "2021111418193754000_8.24.2974.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0968-0004(94)90147-3"
    },
    {
      "key": "2021111418193754000_8.24.2974.4",
      "doi-asserted-by": "crossref",
      "first-page": "1110",
      "DOI": "10.1016/S0021-9258(17)42228-9",
      "article-title": "Endothelin-1 and fibroblast growth factors stimulate the mitogen-activated protein kinase signaling cascade in cardiac myocytes. The potential role of the cascade in the integration of two signaling pathways leading to myocyte hypertrophy.",
      "volume": "269",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418193754000_8.24.2974.5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90182-C"
    },
    {
      "key": "2021111418193754000_8.24.2974.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7681220"
    },
    {
      "key": "2021111418193754000_8.24.2974.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90362-X"
    },
    {
      "key": "2021111418193754000_8.24.2974.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.7.1305"
    },
    {
      "key": "2021111418193754000_8.24.2974.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90503-7"
    },
    {
      "key": "2021111418193754000_8.24.2974.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90015-Q"
    },
    {
      "key": "2021111418193754000_8.24.2974.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90427-8"
    },
    {
      "key": "2021111418193754000_8.24.2974.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00334769"
    },
    {
      "key": "2021111418193754000_8.24.2974.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1411546"
    },
    {
      "key": "2021111418193754000_8.24.2974.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(87)90166-8"
    },
    {
      "key": "2021111418193754000_8.24.2974.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.4.4.492"
    },
    {
      "key": "2021111418193754000_8.24.2974.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/SQB.1991.056.01.007"
    },
    {
      "key": "2021111418193754000_8.24.2974.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.88.21.9392"
    },
    {
      "key": "2021111418193754000_8.24.2974.18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.4.5.495"
    },
    {
      "key": "2021111418193754000_8.24.2974.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0898-6568(93)90078-Z"
    },
    {
      "key": "2021111418193754000_8.24.2974.20",
      "doi-asserted-by": "crossref",
      "first-page": "567",
      "DOI": "10.1128/jb.80.4.567-568.1960",
      "article-title": "Bud position in Saccharomyces cerevisiae.",
      "volume": "80",
      "year": "1960",
      "journal-title": "J. Bacteriology"
    },
    {
      "key": "2021111418193754000_8.24.2974.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.7.1280"
    },
    {
      "key": "2021111418193754000_8.24.2974.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1496375"
    },
    {
      "key": "2021111418193754000_8.24.2974.23",
      "first-page": "2100",
      "article-title": "Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development.",
      "volume": "14",
      "year": "1994",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111418193754000_8.24.2974.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90079-R"
    },
    {
      "key": "2021111418193754000_8.24.2974.25",
      "doi-asserted-by": "crossref",
      "first-page": "325",
      "DOI": "10.1016/0304-4165(66)90387-4",
      "article-title": "Multiplicity of the amino acid permeases in S. cerevisiae.",
      "volume": "127",
      "year": "1966",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "2021111418193754000_8.24.2974.26",
      "first-page": "2952",
      "article-title": "Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae.",
      "volume": "11",
      "year": "1991",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111418193754000_8.24.2974.27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.3.313"
    },
    {
      "key": "2021111418193754000_8.24.2974.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.5.9.1003"
    },
    {
      "key": "2021111418193754000_8.24.2974.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/358417a0"
    },
    {
      "key": "2021111418193754000_8.24.2974.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.2.160"
    },
    {
      "key": "2021111418193754000_8.24.2974.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8385802"
    },
    {
      "key": "2021111418193754000_8.24.2974.32",
      "doi-asserted-by": "crossref",
      "first-page": "4815",
      "DOI": "10.1002/j.1460-2075.1992.tb05587.x",
      "article-title": "The protein kinase homologue Ste20p is required to link the yeast pheromone response G protein \u03b2\u03b3 subunits to downstream signaling components.",
      "volume": "11",
      "year": "1992",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111418193754000_8.24.2974.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8259520"
    },
    {
      "key": "2021111418193754000_8.24.2974.34",
      "unstructured": "Liu, H., Koehler, J. and Fink. G.R. 1994. Suppression of hyphal formation in Candida albicans by mutation of a STE12 homolog. Science (in press)."
    },
    {
      "key": "2021111418193754000_8.24.2974.35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.91.16.7762"
    },
    {
      "key": "2021111418193754000_8.24.2974.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0968-0004(92)90003-R"
    },
    {
      "key": "2021111418193754000_8.24.2974.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.2.452"
    },
    {
      "key": "2021111418193754000_8.24.2974.38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.4.11.1862"
    },
    {
      "key": "2021111418193754000_8.24.2974.39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(92)90177-F"
    },
    {
      "key": "2021111418193754000_8.24.2974.40",
      "unstructured": "Sherman, F., G.R. Fink, and J. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York."
    },
    {
      "key": "2021111418193754000_8.24.2974.41",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.7.1293"
    },
    {
      "key": "2021111418193754000_8.24.2974.42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2880351"
    },
    {
      "key": "2021111418193754000_8.24.2974.43",
      "first-page": "2316",
      "article-title": "Two genes required for cell fusion during yeast conjugation: Evidence for a pheromone-induced surface protein.",
      "volume": "7",
      "year": "1987",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111418193754000_8.24.2974.44",
      "first-page": "749",
      "article-title": "The yeast repeated element sigma contains a hormone-inducible promoter.",
      "volume": "7",
      "year": "1987",
      "journal-title": "Mol. Cell. Bio."
    },
    {
      "key": "2021111418193754000_8.24.2974.45",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90249-3"
    },
    {
      "key": "2021111418193754000_8.24.2974.46",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.2.147"
    },
    {
      "key": "2021111418193754000_8.24.2974.47",
      "first-page": "2069",
      "article-title": "Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases.",
      "volume": "13",
      "year": "1993",
      "journal-title": "Mol. Cell. Biol."
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.8.24.2974",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        15
      ]
    ],
    "date-time": "2021-11-15T02:28:29Z",
    "timestamp": 1636943309000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.8.24.2974"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1994,
        12,
        15
      ]
    ]
  },
  "references-count": 47,
  "journal-issue": {
    "issue": "24",
    "published-print": {
      "date-parts": [
        [
          1994,
          12,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.8.24.2974"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.8.24.2974",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1994,
        12,
        15
      ]
    ]
  }
}