Conserved functional domains of the RNA polymerase III general transcription factor BRF.
10.1101/gad.8.23.2879
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

In Saccharomyces cerevisiae, two components of the RNA polymerase III (Pol III) general transcription factor TFIIIB are the TATA-binding protein (TBP) and the B-related factor (BRF), so called because its amino-terminal half is homologous to the Pol II transcription factor IIB (TFIIB). We have cloned BRF genes from the yeasts Kluyveromyces lactis and Candida albicans. Despite the large evolutionary distance between these species and S. cerevisiae, the BRF proteins are conserved highly. Although the homology is most pronounced in the amino-terminal half, conserved regions also exist in the carboxy-terminal half that is unique to BRF. By assaying for interactions between BRF and other Pol III transcription factors, we show that it is able to bind to the 135-kD subunit of TFIIIC and also to TBP. Surprisingly, in addition to binding the TFIIB-homologous amino-terminal portion of BRF, TBP also interacts strongly with the carboxy-terminal half. Deleting two conserved regions in the BRF carboxy-terminal region abrogates this interaction. Furthermore, TBP mutations that selectively inhibit Pol III transcription in vivo impair interactions between TBP and the BRF carboxy-terminal domain. Finally, we demonstrate that BRF but not TFIIB binds the Pol III subunit C34 and we define a region of C34 necessary for this interaction. These observations provide insights into the roles performed by BRF in Pol III transcription complex assembly.

Bibliography

Khoo, B., Brophy, B., & Jackson, S. P. (1994). Conserved functional domains of the RNA polymerase III general transcription factor BRF. Genes & Development, 8(23), 2879–2890.

Authors 3
  1. B Khoo (first)
  2. B Brophy (additional)
  3. S P Jackson (additional)
References 62 Referenced 104
  1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl. 1991. In Current protocols in molecular biology. John Wiley and Sons/Greene, New York.
  2. {'key': '2021111418191913000_8.23.2879.2', 'first-page': '1243', 'article-title': 'In vitro DNA binding activity of Fos/Jun and BZLF1 but not C/EBP is affected by redox changes.', 'volume': '6', 'year': '1991', 'journal-title': 'Oncogene'} / Oncogene / In vitro DNA binding activity of Fos/Jun and BZLF1 but not C/EBP is affected by redox changes. (1991)
  3. 10.1093/nar/21.5.1289
  4. 10.1073/pnas.90.12.5628
  5. 10.1128/jb.173.7.2250-2255.1991 / J. Bacteriol. / Evolutionary relationships among pathogenic Candida species and relatives. (1991)
  6. {'key': '2021111418191913000_8.23.2879.6', 'first-page': '5181', 'article-title': 'Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC.', 'volume': '11', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC. (1991)
  7. {'key': '2021111418191913000_8.23.2879.7', 'first-page': '942', 'article-title': 'Orientation and topography of RNA polymerase III in transcription complexes.', 'volume': '13', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Orientation and topography of RNA polymerase III in transcription complexes. (1993)
  8. 10.1016/0092-8674(92)90351-C
  9. 10.1073/pnas.90.12.5633
  10. 10.1002/j.1460-2075.1993.tb05936.x / EMBO J. / Unique TATA-binding protein-containing complexes and cofactors involved in transcription by RNA polymerases II and III. (1993)
  11. 10.1101/gad.6.10.1940
  12. 10.1016/0092-8674(92)90039-F
  13. 10.1126/science.8211143
  14. 10.1016/0092-8674(81)90019-2
  15. 10.1093/nar/21.18.4180
  16. 10.1146/annurev.bi.57.070188.004301
  17. 10.1016/0968-0004(91)90070-C
  18. 10.1101/gad.7.6.1021
  19. 10.1101/gad.7.7b.1291
  20. {'key': '2021111418191913000_8.23.2879.20', 'first-page': '189', 'article-title': 'CLUSTAL V: Improved software for multiple sequence alignment.', 'volume': '8', 'year': '1992', 'journal-title': 'Comp. Appl. Biosci.'} / Comp. Appl. Biosci. / CLUSTAL V: Improved software for multiple sequence alignment. (1992)
  21. 10.1038/363744a0
  22. 10.1093/nar/20.24.6451
  23. 10.1016/S0021-9258(18)95736-4 / J. Biol. Chem. / Yeast RNA polymerase C and its subunits. Specific antibodies as structural and functional probes. (1985)
  24. 10.1002/j.1460-2075.1991.tb07958.x / EMBO J. / A conserved heptapeptide restrains the activity of the yeast heat shock transcription factor. (1991)
  25. 10.1126/science.2194289
  26. {'key': '2021111418191913000_8.23.2879.26', 'first-page': '2551', 'article-title': 'Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes.', 'volume': '9', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes. (1989)
  27. 10.1016/0092-8674(90)90739-2
  28. 10.1073/pnas.88.16.7308
  29. 10.1016/0092-8674(92)90399-W
  30. 10.1016/S0021-9258(17)37628-7 / J. Biol. Chem. / Involvement of the basic repeat domain of TATA-binding protein (TBP) in transcription by RNA polymerases I, II, and III. (1994)
  31. 10.1038/365512a0
  32. 10.1016/0022-2836(82)90515-0
  33. 10.1016/0092-8674(91)90321-O
  34. 10.1038/353569a0
  35. 10.1016/0092-8674(92)90397-U
  36. 10.1016/0092-8674(92)90350-L
  37. {'key': '2021111418191913000_8.23.2879.37', 'first-page': '6253', 'article-title': 'Potential RNA polymerase II-induced interactions of transcription factor TFIIB.', 'volume': '13', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Potential RNA polymerase II-induced interactions of transcription factor TFIIB. (1993)
  38. 10.1073/pnas.90.9.4027
  39. 10.1093/nar/21.15.3413
  40. 10.1126/science.2363050
  41. 10.1016/0092-8674(92)90040-J
  42. 10.1016/S0021-9258(18)48334-2 / J. Biol. Chem. / Diverse transcriptional functions of the multisubunit eukaryotic TFIID complex. (1992)
  43. 10.1016/0092-8674(93)90042-O
  44. 10.1038/363741a0
  45. 10.1126/science.8191287
  46. 10.1093/nar/14.19.7767
  47. 10.1007/BF00340712
  48. {'key': '2021111418191913000_8.23.2879.48', 'first-page': '5889', 'article-title': 'Cofractionation of the TATA-binding protein with the RNA polymerase III transcription factor TFIIIB.', 'volume': '20', 'year': '1992', 'journal-title': 'Cell'} / Cell / Cofractionation of the TATA-binding protein with the RNA polymerase III transcription factor TFIIIB. (1992)
  49. 10.1016/0378-1119(88)90005-4
  50. 10.1016/S0021-9258(19)36622-0 / J. Biol. Chem. / An essential and specific subunit of RNA polymerase III (C) is encoded by gene RPC34 in Saccharomyces cerevisiae. (1992)
  51. 10.1126/science.8108728
  52. 10.1016/0092-8674(92)90396-T
  53. 10.1073/pnas.89.23.11292
  54. 10.1016/S0723-2020(11)80099-8
  55. 10.1101/gad.6.8.1542
  56. 10.1016/S0021-9258(19)36839-5 / J. Biol. Chem. / Interaction between a complex of RNA polymerase III subunits and the 70 kDa component of transcription factor IIIB. (1993)
  57. White, R.J. 1994. RNA polymerase III transcription in molecular biology intelligence unit. R.G. Landes Co., Austin, TX.
  58. 10.1016/0092-8674(92)90398-V
  59. 10.1016/0168-9525(92)90136-R
  60. 10.1111/j.1432-1033.1993.tb17626.x / Eur. J. Biochem. / RNA polymerase III. (1993)
  61. {'key': '2021111418191913000_8.23.2879.61', 'first-page': '1111', 'article-title': 'Characterization of a positive regulatory gene, LAC9, that controls induction of the lactose-galactose regulon of Kluyveromyces lactis: Structural and functional relationships to GAL4 of Saccharomyces cerevisiae.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol Cell. Biol.'} / Mol Cell. Biol. / Characterization of a positive regulatory gene, LAC9, that controls induction of the lactose-galactose regulon of Kluyveromyces lactis: Structural and functional relationships to GAL4 of Saccharomyces cerevisiae. (1987)
  62. 10.1126/science.8332911
Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:28 p.m.)
Indexed 1 year, 1 month ago (July 13, 2024, 1:15 p.m.)
Issued 30 years, 9 months ago (Dec. 1, 1994)
Published 30 years, 9 months ago (Dec. 1, 1994)
Published Online 30 years, 9 months ago (Dec. 1, 1994)
Published Print 30 years, 9 months ago (Dec. 1, 1994)
Funders 0

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@article{Khoo_1994, title={Conserved functional domains of the RNA polymerase III general transcription factor BRF.}, volume={8}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.8.23.2879}, DOI={10.1101/gad.8.23.2879}, number={23}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Khoo, B and Brophy, B and Jackson, S P}, year={1994}, month=dec, pages={2879–2890} }