DOI: 10.1101/gad.8.14.1654. Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the adipogenic program in a variety of mouse fibroblastic cells.

Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the adipogenic program in a variety of mouse fibroblastic cells.

10.1101/gad.8.14.1654

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The CCAAT/enhancer-binding protein alpha (C/EBP alpha) has been implicated in the regulation of adipoblast differentiation. In this study we investigate the potential of C/EBP alpha to promote the adipogenic program in a variety of fibroblastic cells. Transduction of the C/EBP alpha gene into eight mouse fibroblastic cell lines by retroviruses and DNA transfection generates adipocyte colonies at variable frequencies. The most dramatic results are obtained with NIH-3T3 cells, in which the percentage of G418-resistant colonies that exhibit the adipocyte morphology is reproducibly > 50% when the C/EBP alpha gene is transduced by retroviruses. The ability to promote the adipogenic program requires the potent transcriptional activation domain of C/EBP alpha and is not observed with C/EBP beta. Paradoxically, in spite of its antimitogenic effects, clonal cell lines that stably express high amounts of C/EBP alpha can readily be generated. Stable expression of C/EBP alpha in BALB/c-3T3 cells dramatically enhances their ability to terminally differentiate into adipocytes. The results demonstrate that C/EBP alpha can efficiently promote the adipogenic program in a variety of mouse fibroblastic cells, including those that have little or no spontaneous capacity to undergo adipogenesis.

Bibliography

Freytag, S. O., Paielli, D. L., & Gilbert, J. D. (1994). Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the adipogenic program in a variety of mouse fibroblastic cells. Genes & Development, 8(14), 1654â1663.

Authors 3

S O Freytag (first)
D L Paielli (additional)
J D Gilbert (additional)

References 34 Referenced 360

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{'key': '2021111418162325000_8.14.1654.22', 'first-page': '1480', 'article-title': 'An attenuator domain is sandwiched by two distinct transactivation domains in the transcription factor C/EBP.', 'volume': '11', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / An attenuator domain is sandwiched by two distinct transactivation domains in the transcription factor C/EBP. (1991)
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10.1101/gad.5.9.1553

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:22 p.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:35 p.m.)
Issued	31 years, 1 month ago (July 15, 1994)
Published	31 years, 1 month ago (July 15, 1994)
Published Online	31 years, 1 month ago (July 15, 1994)
Published Print	31 years, 1 month ago (July 15, 1994)

Funders 0

None

BibTeX

@article{Freytag_1994, title={Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the adipogenic program in a variety of mouse fibroblastic cells.}, volume={8}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.8.14.1654}, DOI={10.1101/gad.8.14.1654}, number={14}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Freytag, S O and Paielli, D L and Gilbert, J D}, year={1994}, month=jul, pages={1654–1663} }

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