Apoptosis or retinoblastoma: alternative fates of photoreceptors expressing the HPV-16 E7 gene in the presence or absence of p53.
10.1101/gad.8.11.1300
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

A transgenic mouse model for retinoblastoma was produced previously by directing SV40 T antigen expression to retinal photoreceptor cells using the promoter of the interstitial retinol-binding protein (IRBP) gene. This gene becomes active prior to the terminal differentiation of photoreceptors. Because T antigen-transforming activity is attributable, at least in part, to the inactivation of the retinoblastoma (pRb) and p53 tumor suppressor proteins, we addressed the role of p53 in the development of retinoblastoma in mice. Transgenic mice expressing HPV-16 E7 under the control of the IRBP promoter were generated to inactivate pRb in photoreceptors while leaving p53 intact. Rather than developing retinoblastomas, the retinas of these mice degenerate due to photoreceptor cell death at a time in development when photoreceptors are normally undergoing terminal differentiation. The dying cells exhibit the histological and ultrastructural features of apoptosis and contain fragmented DNA. p53 is required for the induction of apoptosis in this model, because mice expressing E7 in a p53 nullizygous background develop retinal tumors instead of undergoing retinal degeneration.

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Howes, K. A., Ransom, N., Papermaster, D. S., Lasudry, J. G., Albert, D. M., & Windle, J. J. (1994). Apoptosis or retinoblastoma: alternative fates of photoreceptors expressing the HPV-16 E7 gene in the presence or absence of p53. Genes & Development, 8(11), 1300–1310.

Authors 6
  1. K A Howes (first)
  2. N Ransom (additional)
  3. D S Papermaster (additional)
  4. J G Lasudry (additional)
  5. D M Albert (additional)
  6. J J Windle (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:21 p.m.)
Indexed 10 hours, 46 minutes ago (Aug. 24, 2025, 7:06 p.m.)
Issued 31 years, 2 months ago (June 1, 1994)
Published 31 years, 2 months ago (June 1, 1994)
Published Online 31 years, 2 months ago (June 1, 1994)
Published Print 31 years, 2 months ago (June 1, 1994)
Funders 0

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@article{Howes_1994, title={Apoptosis or retinoblastoma: alternative fates of photoreceptors expressing the HPV-16 E7 gene in the presence or absence of p53.}, volume={8}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.8.11.1300}, DOI={10.1101/gad.8.11.1300}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Howes, K A and Ransom, N and Papermaster, D S and Lasudry, J G and Albert, D M and Windle, J J}, year={1994}, month=jun, pages={1300–1310} }