DOI: 10.1101/gad.8.1.60. unc-101, a gene required for many aspects of Caenorhabditis elegans development and behavior, encodes a clathrin-associated protein.

unc-101, a gene required for many aspects of Caenorhabditis elegans development and behavior, encodes a clathrin-associated protein.

10.1101/gad.8.1.60

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Our genetic analysis indicates that the unc-101 gene of Caenorhabditis elegans is required for many aspects of development and behavior, including negative regulation of vulval differentiation. We have cloned unc-101 and found that it encodes a homolog of the mammalian medium chains of clathrin-associated protein complexes located at the trans-Golgi and the plasma membrane, AP47 and AP50, respectively. Therefore, clathrin-mediated events might contribute to the negative regulation of vulval differentiation. Comparison of sequences, including a full-length sequence of a C. elegans AP50 homolog, reveals that UNC-101 is most closely related to AP47. Mouse AP47 and nematode UNC-101 proteins are functionally equivalent as assayed in transgenic nematodes. We have sequenced the mutant alleles of unc-101 identified in various genetic screens and shown that all but one are deletions or nonsense mutations, suggesting that these alleles severely reduce unc-101 function.

Bibliography

Lee, J., Jongeward, G. D., & Sternberg, P. W. (1994). unc-101, a gene required for many aspects of Caenorhabditis elegans development and behavior, encodes a clathrin-associated protein. Genes & Development, 8(1), 60â73.

Authors 3

J Lee (first)
G D Jongeward (additional)
P W Sternberg (additional)

References 46 Referenced 102

10.1002/j.1460-2075.1988.tb02897.x / EMBO J. / Structural relationships between clathrin assembly proteins from the Golgi and the plasma membrane. (1988)
10.1093/genetics/128.2.251 / Genetics / Multiple functions of let-23, a C. elegans receptor tyrosine kinase gene required for vulval induction. (1991)
10.1038/348693a0
Aroian, R.V., Lesa, G.M. and Sternberg. P.W. 1993a. Mutations in the Caenorhabditis elegans let-23 EGF receptor-like gene define elements important for cell-type specificity and function. EMBO J. 12(13): (in press). (10.1002/j.1460-2075.1994.tb06269.x)
{'key': '2021111418115837000_8.1.60.5', 'first-page': '626', 'article-title': 'Splicing in Caenorhabditis elegans does not require an AG at the 3′ splice acceptor site.', 'volume': '13', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Splicing in Caenorhabditis elegans does not require an AG at the 3′ splice acceptor site. (1993)
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10.1093/genetics/134.4.1119 / Genetics / The Drosophila clathrin heavy chain gene: Clathrin function is essential in a multicellular organism. (1993)
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{'key': '2021111418115837000_8.1.60.11', 'first-page': '4395', 'article-title': 'Comparison of intron-dependent and intron-independent gene expression.', 'volume': '8', 'year': '1988', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Comparison of intron-dependent and intron-independent gene expression. (1988)
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{'key': '2021111418115837000_8.1.60.25', 'first-page': '12182', 'article-title': 'The clathrin coat assembly polypeptide complex: Autophosphorylation and assembly activities.', 'volume': '262', 'year': '1987', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / The clathrin coat assembly polypeptide complex: Autophosphorylation and assembly activities. (1987)
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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:15 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 5, 2025, 8:12 a.m.)
Issued	31 years, 7 months ago (Jan. 1, 1994)
Published	31 years, 7 months ago (Jan. 1, 1994)
Published Online	31 years, 7 months ago (Jan. 1, 1994)
Published Print	31 years, 7 months ago (Jan. 1, 1994)

Funders 0

None

BibTeX

@article{Lee_1994, title={unc-101, a gene required for many aspects of Caenorhabditis elegans development and behavior, encodes a clathrin-associated protein.}, volume={8}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.8.1.60}, DOI={10.1101/gad.8.1.60}, number={1}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Lee, J and Jongeward, G D and Sternberg, P W}, year={1994}, month=jan, pages={60–73} }

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    "10.1101/gad.8.1.60"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.8.1.60",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1994,
        1
      ]
    ]
  }
}