Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID.
10.1101/gad.7.6.1033
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

A Drosophila cDNA encoding the largest TFIID subunit (p230) was isolated using a degenerate oligodeoxynucleotide probe based on an amino acid sequence of the purified protein. The entire cDNA sequence contains an open reading frame encoding a polypeptide of 2068 amino acids, corresponding to a calculated molecular mass of 232 kD. The deduced amino acid sequence showed a strong sequence similarity with the protein encoded by a human gene (CCG1) implicated in cell cycle progression through G1, suggesting that p230 may be a target for cell cycle regulatory factors. The recombinant protein expressed in Sf9 cells via a baculovirus vector interacts directly with the TATA box-binding subunit of TFIID (TFIID tau or TBP) from Drosophila, human, and yeast. Surprisingly, recombinant p230 inhibits the TATA box-binding activity and function of TFIID tau, suggesting that p230 interactions with TFIID tau, and possible modulations thereof by other factors may play an important role in TFIID function.

Bibliography

Kokubo, T., Gong, D. W., Yamashita, S., Horikoshi, M., Roeder, R. G., & Nakatani, Y. (1993). Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID. Genes & Development, 7(6), 1033–1046.

Authors 6
  1. T Kokubo (first)
  2. D W Gong (additional)
  3. S Yamashita (additional)
  4. M Horikoshi (additional)
  5. R G Roeder (additional)
  6. Y Nakatani (additional)
References 84 Referenced 69
  1. Ashburner, M. 1989. Drosophila: A laboratory handbook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  2. 10.3109/10425179209020804 / DNA Sequencing / A homologue of the Drosophila female sterile homeotic (fsh) gene in the class II region of the human MHC. (1992)
  3. 10.1101/gad.6.2.166
  4. 10.1016/0092-8674(92)90443-G
  5. 10.1002/jcp.1040840308
  6. {'key': '2021111418091208000_7.6.1033.6', 'first-page': '2487', 'article-title': 'Sequence requirement for synthetic peptide mediated translocation to the nucleus.', 'volume': '9', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Sequence requirement for synthetic peptide mediated translocation to the nucleus. (1989)
  7. 10.1016/0092-8674(90)90511-C
  8. 10.1016/0092-8674(91)90167-W
  9. 10.1016/0092-8674(88)90144-4
  10. {'key': '2021111418091208000_7.6.1033.10', 'first-page': '1879', 'article-title': 'A presumptive helicase (MOT1 gene product) affects gene expression and is required for viability in the yeast Saccharomyces cerevisiae.', 'volume': '12', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / A presumptive helicase (MOT1 gene product) affects gene expression and is required for viability in the yeast Saccharomyces cerevisiae. (1992)
  11. 10.1016/0092-8674(81)90019-2
  12. 10.1002/j.1460-2075.1992.tb05507.x / EMBO J. / Two distinct yeast transcriptional activators require the function of the GCN5 protein to promote normal levels of transcription. (1992)
  13. 10.1002/j.1460-2075.1990.tb08283.x / EMBO J. / The cell type-specific octamer transcription factor OTF-2 has two domains required for the activation of transcription. (1990)
  14. 10.1016/0092-8674(92)90129-Z
  15. 10.1016/0092-8674(91)90166-V
  16. 10.1016/S0959-437X(05)80279-5
  17. 10.1038/346245a0
  18. Harlow, E. and D. Lane. 1988. Antibodies: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  19. 10.1016/0012-1606(89)90094-8
  20. 10.1093/nar/20.10.2603
  21. 10.1073/pnas.81.19.6004
  22. 10.1101/gad.6.12a.2288
  23. 10.1016/0092-8674(90)90682-5
  24. 10.1038/346387a0
  25. 10.1016/S0092-8674(88)80011-4
  26. 10.1016/0092-8674(88)90118-3
  27. 10.1073/pnas.86.13.4843
  28. 10.1038/341299a0
  29. 10.1073/pnas.88.12.5124
  30. 10.1016/0092-8674(91)90028-W
  31. 10.1038/344830a0
  32. {'key': '2021111418091208000_7.6.1033.32', 'first-page': '4503', 'article-title': 'Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae.', 'volume': '12', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae. (1992)
  33. 10.1126/science.2006421
  34. 10.1002/j.1460-2075.1988.tb02973.x / EMBO J. / Four mating-type genes control sexual differentiation in the fission yeast. (1988)
  35. Identification of TFIID components required for transcriptional activation by USF. J. Biol. Chem. and (in press).. Kokubo, T., . Takada, R. . Yamashita, S. . Gong, D.-W. . Roeder, R.G. . Horikoshi, M. . Nakatani. Y. .
  36. 10.1016/S0021-9258(18)48058-1 / J. Biol. Chem. / Substrate specificity determinants for casein kinase II as deduced from studies with synthetic peptides. (1987)
  37. 10.1016/0076-6879(87)54085-X
  38. 10.1101/gad.6.9.1707
  39. 10.1073/pnas.88.7.2687
  40. {'key': '2021111418091208000_7.6.1033.40', 'first-page': '1893', 'article-title': 'An essential Saccharomyces cerevisiae gene homologous to SNF2 encodes a helicase-related protein in a new family.', 'volume': '12', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / An essential Saccharomyces cerevisiae gene homologous to SNF2 encodes a helicase-related protein in a new family. (1992)
  41. 10.1016/0092-8674(91)90188-5
  42. LeGendre, N. and P. Matsudaira. 1989. Purification of proteins and peptides by SDS-PAGE. Academic Press, San Diego, CA.
  43. 10.1101/gad.5.12b.2441
  44. 10.1016/0092-8674(92)90311-Y
  45. 10.1016/S0021-9258(19)83616-5 / J. Biol. Chem. / Expression of thymidine kinase and dihydrofolate reductase genes in mammalian ts mutants of the cell cycle. (1985)
  46. 10.1038/344517a0
  47. 10.1002/j.1460-2075.1992.tb05032.x / EMBO J. / Casein kinase II phosphorylation increases the rate of serum response factor-binding site exchange. (1992)
  48. 10.1073/pnas.87.23.9148
  49. {'key': '2021111418091208000_7.6.1033.49', 'first-page': '4028', 'article-title': 'Factors involved in specific transcription by mammalian RNA polymerase II: Purification, genetic specificity, and TATA box-promoter interactions of TFIID.', 'volume': '8', 'year': '1988', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Factors involved in specific transcription by mammalian RNA polymerase II: Purification, genetic specificity, and TATA box-promoter interactions of TFIID. (1988)
  50. 10.1073/pnas.87.11.4289
  51. 10.1038/348086a0
  52. 10.1016/0092-8674(92)90192-F
  53. 10.1126/science.2363050
  54. 10.1016/0092-8674(85)90306-X
  55. {'key': '2021111418091208000_7.6.1033.55', 'first-page': '4809', 'article-title': 'The conserved carboxy-terminal domain of Saccharomyces cerevisiae TFIID is sufficient to support normal cell growth.', 'volume': '11', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The conserved carboxy-terminal domain of Saccharomyces cerevisiae TFIID is sufficient to support normal cell growth. (1991)
  56. 10.1101/gad.5.11.1935
  57. 10.1016/S0021-9258(18)48334-2 / J. Biol. Chem. / Diverse transcriptional functions of the multisubunit eukaryotic TFIID complex. (1992)
  58. 10.1016/0168-9525(91)90279-Y
  59. 10.1016/0968-0004(91)90164-Q
  60. 10.1093/nar/15.19.8112
  61. 10.1038/354245a0
  62. 10.1016/0092-8674(85)90021-2
  63. 10.1073/pnas.82.13.4394
  64. 10.1002/j.1460-2075.1988.tb02996.x / EMBO J. / Molecular cloning of the cDNA of human X chromosomal gene (CCG1) which complements the temperature-sensitive G1 mutants, tsBN462 and ts13, of the BHK cell line. (1988)
  65. {'key': '2021111418091208000_7.6.1033.65', 'first-page': '3317', 'article-title': 'The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.', 'volume': '11', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. (1991)
  66. 10.1038/354241a0
  67. 10.1073/pnas.89.24.12028
  68. 10.1016/0092-8674(89)90176-1
  69. 10.1073/pnas.87.13.4917
  70. 10.1038/345783a0
  71. 10.1073/pnas.89.24.11809
  72. 10.1016/0092-8674(92)90191-E
  73. 10.1101/gad.5.12a.2212
  74. 10.1016/0092-8674(92)90218-2
  75. 10.1101/gad.5.5.880
  76. 10.1016/S0021-9258(18)53769-8 / J. Biol. Chem. / Direct interaction between the transcriptional activation domain of human p53 and the TATA box-binding protein. (1993)
  77. 10.1002/j.1460-2075.1991.tb07928.x / EMBO J. / Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box. (1991)
  78. 10.1002/j.1460-2075.1992.tb05280.x / EMBO J. / The nucleolar transcription factor mUBF is phosphorylated by casein kinase II in the C-terminal hyperacidic tail which is essential for transactivation. (1992)
  79. {'key': '2021111418091208000_7.6.1033.79', 'first-page': '77', 'article-title': 'Microinjection of RNA polymerase II corrects the temperature-sensitive defect of tsAF8 cells.', 'volume': '60', 'year': '1984', 'journal-title': 'Mol. Cell. Biochem.'} / Mol. Cell. Biochem. / Microinjection of RNA polymerase II corrects the temperature-sensitive defect of tsAF8 cells. (1984)
  80. 10.1093/nar/17.3.1197
  81. 10.1073/pnas.89.7.2839
  82. 10.1002/j.1460-2075.1991.tb07710.x / EMBO J. / Requirement for acidic amino acid residues immediately N-terminal to the conserved domain of Saccharomyces cerevisiae TFIID. (1991)
  83. 10.1101/gad.6.10.1964
  84. 10.1016/0092-8674(88)90574-0
Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:11 p.m.)
Indexed 1 year ago (Aug. 3, 2024, 3:39 p.m.)
Issued 32 years, 3 months ago (June 1, 1993)
Published 32 years, 3 months ago (June 1, 1993)
Published Online 32 years, 3 months ago (June 1, 1993)
Published Print 32 years, 3 months ago (June 1, 1993)
Funders 0

None

@article{Kokubo_1993, title={Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.6.1033}, DOI={10.1101/gad.7.6.1033}, number={6}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Kokubo, T and Gong, D W and Yamashita, S and Horikoshi, M and Roeder, R G and Nakatani, Y}, year={1993}, month=jun, pages={1033–1046} }