GAL4 disrupts a repressing nucleosome during activation of GAL1 transcription in vivo.
10.1101/gad.7.5.857
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

Photofootprinting in vivo of GAL1 reveals an activation-dependent pattern between the UASG and the TATA box, in a sequence not required for transcriptional activation by GAL4. The pattern results from a nucleosome whose position depends on sequences within the UASG. In the wild-type gene, activation by GAL4 and derivatives disrupts this nucleosome. This activity is independent of interactions with DNA-bound core transcription factors and is proportional to the strength of the activator. Presence of the nucleosome correlates with low basal transcription levels under various conditions, suggesting a role in limiting basal expression. We propose a role for the GAL4 activation domain in displacing a nucleosome and suggest that this is part of the mechanism by which GAL4 activates transcription in vivo.

Bibliography

Axelrod, J. D., Reagan, M. S., & Majors, J. (1993). GAL4 disrupts a repressing nucleosome during activation of GAL1 transcription in vivo. Genes & Development, 7(5), 857–869.

Authors 3
  1. J D Axelrod (first)
  2. M S Reagan (additional)
  3. J Majors (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:10 p.m.)
Indexed 1 month, 3 weeks ago (July 1, 2025, 5:12 p.m.)
Issued 32 years, 3 months ago (May 1, 1993)
Published 32 years, 3 months ago (May 1, 1993)
Published Online 32 years, 3 months ago (May 1, 1993)
Published Print 32 years, 3 months ago (May 1, 1993)
Funders 0

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@article{Axelrod_1993, title={GAL4 disrupts a repressing nucleosome during activation of GAL1 transcription in vivo.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.5.857}, DOI={10.1101/gad.7.5.857}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Axelrod, J D and Reagan, M S and Majors, J}, year={1993}, month=may, pages={857–869} }