p105 and p98 precursor proteins play an active role in NF-kappa B-mediated signal transduction.
10.1101/gad.7.4.705
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The Rel/NF-kappa B family of transcription factors is composed of two distinct subgroups, proteins that undergo proteolytic processing and contain SWI6/ankyrin repeats in their carboxyl termini (p105, p98), and those without such repeats that do not require processing (p65, c-Rel, RelB, and Dorsal). We demonstrate that the p105 and p98 precursors share functional properties with the I kappa B proteins, which also contain SWI6/ankyrin repeats. Both p105 and p98 were found to form stable complexes with other Rel/NF-kappa B family members, including p65 and c-Rel. Association with the precursors is sufficient for cytoplasmic retention of either p65 or c-Rel, both of which are otherwise nuclear. These complexes undergo stimulus-responsive processing to produce active p50/c-Rel and p55/c-Rel complexes. These observations suggest a second pathway leading to NF-kappa B induction, in which processing of the precursors rather than phosphorylation of I kappa B plays a major role.

Bibliography

Mercurio, F., DiDonato, J. A., Rosette, C., & Karin, M. (1993). p105 and p98 precursor proteins play an active role in NF-kappa B-mediated signal transduction. Genes & Development, 7(4), 705–718.

Authors 4
  1. F Mercurio (first)
  2. J A DiDonato (additional)
  3. C Rosette (additional)
  4. M Karin (additional)
References 60 Referenced 227
  1. 10.1038/332166a0
  2. {'key': '2021111418082804000_7.4.705.2', 'first-page': '63', 'article-title': 'The inducible transcriptional activator NF-κB: Regulation by distinct protein subunits.', 'volume': '1072', 'year': '1991', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta / The inducible transcriptional activator NF-κB: Regulation by distinct protein subunits. (1991)
  3. 10.1126/science.3140380
  4. 10.1016/0092-8674(88)90382-0
  5. 10.1101/gad.3.11.1689
  6. Baeuerke, P.A. and D. Baltimore. 1991. The physiology of the NF-κB transcription factor. In The horomonal control regulation of gene transcription (ed. P. Cohen and J.G. Foulkes), pp. 423–446. Elsevier Science Publishers, Amsterdam. (10.1016/B978-0-444-81382-4.50026-8)
  7. 10.1016/0092-8674(90)90146-6
  8. 10.1101/gad.6.10.1899
  9. 10.1002/j.1460-2075.1991.tb04994.x / EMBO J. / Cytoplasmic retention, DNA-binding and processing of the NF-κB p50 precursor are controlled by a small region in its C-terminus. (1991)
  10. {'key': '2021111418082804000_7.4.705.10', 'first-page': '135', 'article-title': 'NF-κB and related proteins: Rel/dorsal homologies meet ankyrin-like repeats.', 'volume': '3', 'year': '1992', 'journal-title': 'Trends Biol. Sci.'} / Trends Biol. Sci. / NF-κB and related proteins: Rel/dorsal homologies meet ankyrin-like repeats. (1992)
  11. 10.1038/348076a0
  12. {'key': '2021111418082804000_7.4.705.12', 'first-page': '685', 'article-title': 'A novel mitogen-inducible gene product related to p50/p105-NF-κB participates in transactivation through a κB site.', 'volume': '12', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / A novel mitogen-inducible gene product related to p50/p105-NF-κB participates in transactivation through a κB site. (1992)
  13. {'key': '2021111418082804000_7.4.705.13', 'first-page': '935', 'article-title': 'A human rel proto-oncogene cDNA containing an Alu fragment as a potential coding exon.', 'volume': '4', 'year': '1989', 'journal-title': 'Oncogene'} / Oncogene / A human rel proto-oncogene cDNA containing an Alu fragment as a potential coding exon. (1989)
  14. 10.1128/jvi.66.6.3758-3767.1992 / J. Virol. / p105, the NF-κB p50 precursor protein, is one of the cellular proteins complexed with the v-Rel oncoprotein in transformed chicken spleen cells. (1992)
  15. {'key': '2021111418082804000_7.4.705.15', 'first-page': '1109', 'article-title': 'Avian reticuloendotheliosis virus-transformed lymphoid cells contain multiple pp59v-rel complexes.', 'volume': '5', 'year': '1990', 'journal-title': 'Oncogene'} / Oncogene / Avian reticuloendotheliosis virus-transformed lymphoid cells contain multiple pp59v-rel complexes. (1990)
  16. 10.1126/science.1891714
  17. 10.1093/nar/11.5.1475
  18. 10.1038/354395a0
  19. 10.1038/344678a0
  20. 10.1016/0092-8674(90)90276-K
  21. 10.1016/0168-9525(91)90421-L / Trends Genet / Malignant transformation by mutant Rel proteins. (1991)
  22. Harlow, E. and D. Lane. 1988. Cell staining. In Antibodies: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  23. 10.1016/0092-8674(91)90022-Q
  24. 10.1073/pnas.89.6.2489
  25. 10.1016/0092-8674(92)90083-O
  26. 10.1016/0092-8674(92)90162-6
  27. 10.1016/0092-8674(92)90082-N
  28. 10.1096/fasebj.6.8.1317309 / FASEB J. / Signal transduction from cell surface to nucleus in development and disease. (1992)
  29. 10.1101/gad.5.8.1464
  30. 10.1016/0092-8674(90)90275-J
  31. 10.1016/0092-8674(89)90833-7
  32. 10.1016/0042-6822(90)90195-W
  33. 10.1002/j.1460-2075.1991.tb07708.x / EMBO J. / Inhibition of transcription factors belonging to the rel/NF-κB family by a transdominant negative mutant. (1991)
  34. 10.1016/0003-9861(86)90398-X
  35. 10.1089/dna.1992.11.523
  36. 10.1073/pnas.88.3.966
  37. 10.1073/pnas.87.24.10028
  38. {'key': '2021111418082804000_7.4.705.38', 'first-page': '677', 'article-title': 'Viral rel and cellular rel associate with cellular proteins in transformed and normal cells.', 'volume': '4', 'year': '1989', 'journal-title': 'Oncogene'} / Oncogene / Viral rel and cellular rel associate with cellular proteins in transformed and normal cells. (1989)
  39. 10.1016/0092-8674(91)90285-7
  40. 10.1016/0092-8674(91)90320-X
  41. 10.1016/0092-8674(90)90347-H
  42. 10.1073/pnas.89.5.1529
  43. {'key': '2021111418082804000_7.4.705.43', 'first-page': '566', 'article-title': 'A novel T-cell trans-activator that recognizes a phorbol-esterinducibille element of the interleukin-2 promotor.', 'volume': '2', 'year': '1990', 'journal-title': 'New Biol.'} / New Biol. / A novel T-cell trans-activator that recognizes a phorbol-esterinducibille element of the interleukin-2 promotor. (1990)
  44. 10.1016/0092-8674(92)90353-E
  45. 10.1038/350625a0
  46. 10.1126/science.2006423
  47. 10.1002/bies.950140204
  48. {'key': '2021111418082804000_7.4.705.48', 'first-page': '674', 'article-title': 'RelB, a new Rel family transcription activator that can interact with p50-NF-κB.', 'volume': '12', 'year': '1992', 'journal-title': 'Mol. Cell Biol.'} / Mol. Cell Biol. / RelB, a new Rel family transcription activator that can interact with p50-NF-κB. (1992)
  49. 10.1038/352733a0
  50. {'key': '2021111418082804000_7.4.705.50', 'first-page': '2424', 'article-title': 'In vitro activation and nuclear translocation of NF-κB catalyzed by cyclic AMP-dependent protein kinase and protein kinase C.', 'volume': '9', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / In vitro activation and nuclear translocation of NF-κB catalyzed by cyclic AMP-dependent protein kinase and protein kinase C. (1989)
  51. {'key': '2021111418082804000_7.4.705.51', 'first-page': '4730', 'article-title': 'p59v-Rel, the transforming protein of reticuloendotheliosis virus, is complexed with at least four other proteins in transformed chicken lymphoid cells.', 'volume': '2', 'year': '1988', 'journal-title': 'J. Virol.'} / J. Virol. / p59v-Rel, the transforming protein of reticuloendotheliosis virus, is complexed with at least four other proteins in transformed chicken lymphoid cells. (1988)
  52. {'key': '2021111418082804000_7.4.705.52', 'first-page': '103', 'article-title': 'Detection and characterization of the protein encoded by the chicken c-Rel protooncogene.', 'volume': '2', 'year': '1988', 'journal-title': 'Oncogene Res.'} / Oncogene Res. / Detection and characterization of the protein encoded by the chicken c-Rel protooncogene. (1988)
  53. 10.1073/pnas.80.20.6229
  54. 10.1126/science.3118464
  55. {'key': '2021111418082804000_7.4.705.55', 'first-page': '4067', 'article-title': 'κB site-dependent activation of the interleukin-2 receptor α-chain gene promotor by human c-Rel.', 'volume': '12', 'year': '1992', 'journal-title': 'Mol. Cell Biol.'} / Mol. Cell Biol. / κB site-dependent activation of the interleukin-2 receptor α-chain gene promotor by human c-Rel. (1992)
  56. 10.1073/pnas.85.8.2479
  57. 10.1101/gad.4.11.1975
  58. 10.1002/j.1460-2075.1991.tb07707.x / EMBO J. / NF-κB contacts DNA by a heterodimer of the p50 and p65 subunit. (1991)
  59. 10.1128/jvi.52.1.172-182.1984 / J. Virol. / Nucleic acid sequences of the oncogene v-rel in reticulendotheliosis virus strain T and its cellular homolog, the proto-oncogene c-rel. (1984)
  60. 10.1016/0092-8674(90)90806-P
Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:12 p.m.)
Indexed 1 month, 4 weeks ago (June 26, 2025, 3:02 p.m.)
Issued 32 years, 4 months ago (April 1, 1993)
Published 32 years, 4 months ago (April 1, 1993)
Published Online 32 years, 4 months ago (April 1, 1993)
Published Print 32 years, 4 months ago (April 1, 1993)
Funders 0

None

@article{Mercurio_1993, title={p105 and p98 precursor proteins play an active role in NF-kappa B-mediated signal transduction.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.4.705}, DOI={10.1101/gad.7.4.705}, number={4}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Mercurio, F and DiDonato, J A and Rosette, C and Karin, M}, year={1993}, month=apr, pages={705–718} }