A null c-myc mutation causes lethality before 10.5 days of gestation in homozygotes and reduced fertility in heterozygous female mice.
10.1101/gad.7.4.671
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

To directly assess c-myc function in cellular proliferation, differentiation, and embryogenesis, we have used homologous recombination in embryonic stem cells to generate both heterozygous and homozygous c-myc mutant ES cell lines. The mutation is a null allele at the protein level. Mouse chimeras from seven heterozygous cell lines transmitted the mutant allele to their offspring. The analysis of embryos from two clones has shown that the mutation is lethal in homozygotes between 9.5 and 10.5 days of gestation. The embryos are generally smaller and retarded in development compared with their littermates. Pathologic abnormalities include the heart, pericardium, neural tube, and delay or failure in turning of the embryo. Heterozygous females have reduced fertility owing to embryonic resorption before 9.5 days of gestation in 14% of implanted embryos. c-Myc protein is necessary for embryonic survival beyond 10.5 days of gestation; however, it appears to be dispensable for cell division both in ES cell lines and in the embryo before that time.

Bibliography

Davis, A. C., Wims, M., Spotts, G. D., Hann, S. R., & Bradley, A. (1993). A null c-myc mutation causes lethality before 10.5 days of gestation in homozygotes and reduced fertility in heterozygous female mice. Genes & Development, 7(4), 671–682.

Authors 5
  1. A C Davis (first)
  2. M Wims (additional)
  3. G D Spotts (additional)
  4. S R Hann (additional)
  5. A Bradley (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 1 year, 6 months ago (Feb. 14, 2024, 4:23 p.m.)
Indexed 3 days, 6 hours ago (Aug. 23, 2025, 1:03 a.m.)
Issued 32 years, 4 months ago (April 1, 1993)
Published 32 years, 4 months ago (April 1, 1993)
Published Online 32 years, 4 months ago (April 1, 1993)
Published Print 32 years, 4 months ago (April 1, 1993)
Funders 0

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@article{Davis_1993, title={A null c-myc mutation causes lethality before 10.5 days of gestation in homozygotes and reduced fertility in heterozygous female mice.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.4.671}, DOI={10.1101/gad.7.4.671}, number={4}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Davis, A C and Wims, M and Spotts, G D and Hann, S R and Bradley, A}, year={1993}, month=apr, pages={671–682} }