DOI: 10.1101/gad.7.3.479. JunB differs from c-Jun in its DNA-binding and dimerization domains, and represses c-Jun by formation of inactive heterodimers.

JunB differs from c-Jun in its DNA-binding and dimerization domains, and represses c-Jun by formation of inactive heterodimers.

10.1101/gad.7.3.479

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

JunB differs considerably from c-Jun in its ability to activate AP-1-responsive genes and induce oncogenic transformation. We demonstrate that the decreased ability of JunB to activate gene expression is the result of a small number of amino acid changes between its DNA-binding and dimerization motifs and the corresponding regions of c-Jun. These changes lead to a 10-fold decrease in the DNA-binding activity of JunB. JunB can be converted into a c-Jun-like activator by substituting four amino acids in its DNA-binding and dimerization motifs with the corresponding c-Jun sequences. JunB can also attenuate trans-activation by c-Jun, an activity mediated by its leucine zipper. This ability depends on two glycine residues that decrease the stability of the JunB leucine zipper, resulting in decreased homodimerization and increased heterodimerization. These results illustrate how small changes in primary structure, including chemically conservative changes, can result in functional divergence of two highly related transcriptional regulators.

Bibliography

Deng, T., & Karin, M. (1993). JunB differs from c-Jun in its DNA-binding and dimerization domains, and represses c-Jun by formation of inactive heterodimers. Genes & Development, 7(3), 479â490.

Authors 2

T Deng (first)
M Karin (additional)

References 58 Referenced 246

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{'key': '2021111418074345000_7.3.479.22', 'first-page': '606', 'article-title': 'The metal responsive elements act as positive modulators of human metallothionein-IIλ enhancer activity.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The metal responsive elements act as positive modulators of human metallothionein-IIλ enhancer activity. (1987)
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Dates

Type	When
Created	18 years, 3 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:08 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:12 a.m.)
Issued	32 years, 6 months ago (March 1, 1993)
Published	32 years, 6 months ago (March 1, 1993)
Published Online	32 years, 6 months ago (March 1, 1993)
Published Print	32 years, 6 months ago (March 1, 1993)

Funders 0

None

BibTeX

@article{Deng_1993, title={JunB differs from c-Jun in its DNA-binding and dimerization domains, and represses c-Jun by formation of inactive heterodimers.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.3.479}, DOI={10.1101/gad.7.3.479}, number={3}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Deng, T and Karin, M}, year={1993}, month=mar, pages={479–490} }

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        [
          1993,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.7.3.479"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.7.3.479",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1993,
        3
      ]
    ]
  }
}