Abstract
The HAT4 gene from the plant Arabidopsis thaliana encodes a homeo domain protein that contains a leucine zipper motif. Homeo domain-leucine zipper (HD-Zip) proteins have not been found in animal systems, suggesting that HAT4 may define a new family of transcription factors that regulate higher plant development. To explore this possibility, functional studies of HAT4 were carried out in yeast and in transgenic plants. Point mutants of HAT4 isolated in yeast define functionally critical residues within the HD-Zip domain, many of which correspond to highly conserved positions in known homeo domains and leucine zippers. Transgenic plants bearing constructs that alter HAT4 expression exhibit a series of interesting developmental phenotypes, including changes in morphology and developmental rate. Thus, the HAT4 gene of Arabidopsis encodes an HD-Zip protein that functions as a novel developmental regulator.
References
59
Referenced
103
10.1016/0092-8674(92)90100-Q
10.1016/0092-8674(90)90587-5
10.1016/0092-8674(85)90246-6
10.1016/0092-8674(87)90585-X
10.1126/science.2255907
10.1126/science.2884726
10.1016/0168-9525(90)90253-3
/ Trends Genet. / The structure of the homeodomain and its functional implications. (1990)10.1038/334721a0
10.1126/science.1496375
10.1016/0092-8674(92)90079-R
10.1016/0168-9525(92)90021-U
10.1016/0092-8674(90)90492-W
10.1016/0092-8674(81)90432-3
10.1126/science.2147779
{'key': '2021111418074349000_7.3.367.15', 'first-page': '163', 'article-title': 'Transformation of intact yeast cells treated with alkali cations.', 'volume': '53', 'year': '1983', 'journal-title': 'J. Bacteriol.'}
/ J. Bacteriol. / Transformation of intact yeast cells treated with alkali cations. (1983)10.1016/0168-9525(92)90020-5
10.1016/S0065-2660(08)60029-2
10.1016/0092-8674(90)90453-L
{'key': '2021111418074349000_7.3.367.19', 'first-page': '45', 'article-title': 'The complex syndrome of ttg mutants.', 'volume': '18', 'year': '1981', 'journal-title': 'Arabidopsis Inf. Serv.'}
/ Arabidopsis Inf. Serv. / The complex syndrome of ttg mutants. (1981)10.1126/science.3289117
{'key': '2021111418074349000_7.3.367.21', 'first-page': '260', 'article-title': 'Primary structure of the Saccharomyces cerevisiae GAL4 gene.', 'volume': '4', 'year': '1984', 'journal-title': 'Mol. Cell. Biol.'}
/ Mol. Cell. Biol. / Primary structure of the Saccharomyces cerevisiae GAL4 gene. (1984)10.1001/jama.1992.03480110100042
10.1126/science.1465611
10.1073/pnas.86.18.7092
10.1038/334631a0
10.1101/gad.5.3.484
10.1007/BF00019223
10.1016/0092-8674(92)90471-N
10.1126/science.2667136
10.1111/j.1574-6968.1990.tb04041.x
10.1016/0092-8674(91)90292-7
10.1016/0092-8674(92)90159-A
10.1016/0092-8674(91)90523-2
10.1126/science.2911757
10.1126/science.1948029
10.1038/346329a0
- Richards, E. 1988. Preparation of genomic DNA from plant tissue. In Current protocols in molecular biology (ed. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, and J.A. Smith), pp. 2.3.1–3. Greene/Wiley-Interscience, New York.
10.1002/j.1460-2075.1991.tb07703.x
/ EMBO J. / A novel class of plant proteins containing a homeodomain with a closely linked leucine zipper motif. (1991)- Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
10.1007/BF01953055
- Schena, M. 1990. “Mammalian glucocorticoid receptor action in yeast.” Ph.D. thesis, University of California at San Francisco, San Francisco, CA.
10.1073/pnas.89.9.3894
10.1126/science.3043665
10.1101/gad.3.10.1590
10.1016/0076-6879(91)94029-C
10.1073/pnas.88.23.10421
10.1007/BF00340712
{'key': '2021111418074349000_7.3.367.48', 'first-page': '25', 'article-title': 'The structure and function of the homeodomain.', 'volume': '989', 'year': '1989', 'journal-title': 'Biochim. Biophys. Acta'}
/ Biochim. Biophys. Acta / The structure and function of the homeodomain. (1989)10.1016/S1046-2023(05)80158-6
10.1016/0968-0004(89)90145-X
10.1073/pnas.85.15.5536
10.1126/science.2683088
10.1038/350241a0
10.1126/science.1846704
10.1101/gad.5.9.1553
10.1126/science.1925541
10.1016/0092-8674(91)90526-5
10.1038/346035a0
{'key': '2021111418074349000_7.3.367.59', 'first-page': '1985', 'article-title': 'Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.', 'volume': '4', 'year': '1984', 'journal-title': 'Mol. Cell Biol.'}
/ Mol. Cell Biol. / Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. (1984)
Dates
Type | When |
---|---|
Created | 18 years, 2 months ago (June 5, 2007, 5:15 p.m.) |
Deposited | 3 years, 9 months ago (Nov. 14, 2021, 9:08 p.m.) |
Indexed | 4 weeks ago (Aug. 5, 2025, 8:37 a.m.) |
Issued | 32 years, 6 months ago (March 1, 1993) |
Published | 32 years, 6 months ago (March 1, 1993) |
Published Online | 32 years, 6 months ago (March 1, 1993) |
Published Print | 32 years, 6 months ago (March 1, 1993) |
@article{Schena_1993, title={The HAT4 gene of Arabidopsis encodes a developmental regulator.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.3.367}, DOI={10.1101/gad.7.3.367}, number={3}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Schena, M and Lloyd, A M and Davis, R W}, year={1993}, month=mar, pages={367–379} }