DOI: 10.1101/gad.7.11.2235. Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex.

Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex.

10.1101/gad.7.11.2235

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The basal factor TFIIA has been shown to act early during initiation in both the mammalian and yeast transcription systems, but a TFIIA-like activity has not been identified in Drosophila. While characterizing the Drosophila TFIID complex, we discovered that a 30-kD protein that cofractionated with dTFIID was homologous to the previously identified, large subunit of yeast TFIIA. Here, we report the cloning and biochemical characterization of Drosophila TFIIA-L. Coimmunoprecipitation studies with anti-dTBP, anti-dTFIIA-L, and anti-TAF antibodies indicated a tight association of the endogenous dTFIIA and dTFIID. However, dTFIIA could be dissociated from dTFIID under conditions that did not elute the TAFs, and the eluted material had mobility shift and transcriptional activities associated with TFIIA. Peptide sequence and Western analysis with antibodies raised against the amino- and carboxy-terminal portions of recombinant dTFIIA-L revealed that a precursor 48-kD species was cleaved in vivo, giving rise to the 30- and 20-kD subunits of dTFIIA that remain associated with each other and with dTFIID. Protein-protein interaction assays identified dTBP and dTAFII110 as targets for binding TFIIA in the TFIID complex. These results suggest that TFIIA may form a specific complex with both TAFs and other components of the transcriptional machinery during formation of the initiation complex.

Bibliography

Yokomori, K., Admon, A., Goodrich, J. A., Chen, J. L., & Tjian, R. (1993). Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex. Genes & Development, 7(11), 2235â2245.

Authors 5

K Yokomori (first)
A Admon (additional)
J A Goodrich (additional)
J L Chen (additional)
R Tjian (additional)

References 37 Referenced 91

{'key': '2021111418110922000_7.11.2235.1', 'first-page': '461', 'article-title': 'Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF.', 'volume': '8', 'year': '1992', 'journal-title': 'Nature'} / Nature / Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF. (1992)
{'key': '2021111418110922000_7.11.2235.2', 'first-page': '1602', 'article-title': 'RAP30/RAP74: A general initiation factor that binds to RNA polymerase II.', 'volume': '8', 'year': '1988', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / RAP30/RAP74: A general initiation factor that binds to RNA polymerase II. (1988)
{'key': '2021111418110922000_7.11.2235.3', 'first-page': '413', 'article-title': 'Factors involved in transcription by mammalian RNA polymerase II: Purification and analysis of transcription factor IIA and identification of transcription factor IIJ.', 'volume': '12', 'year': '1992', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Factors involved in transcription by mammalian RNA polymerase II: Purification and analysis of transcription factor IIA and identification of transcription factor IIJ. (1992)
10.1101/gad.3.11.1677
{'key': '2021111418110922000_7.11.2235.5', 'first-page': '563', 'article-title': 'Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation.', 'volume': '55', 'year': '1991', 'journal-title': 'Cell'} / Cell / Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation. (1991)
10.1038/363176a0
10.1038/355464a0
10.1126/science.1529339
10.1016/S0021-9258(18)45947-9 / J. Biol. Chem. / Factors involved in specific transcription initiation by RNA polymerase II: Identification and characterization of factor IIH. (1992)
10.1038/352689a0
10.1038/362179a0
10.1016/0092-8674(90)90682-5
10.1016/0092-8674(93)90664-C
10.1016/S0021-9258(18)31585-0 / J. Biol. Chem. / Factors involved in specific transcription by mammalian RNA polymerase II: Purification and functional analysis of general transcription factor IIE. (1991)
10.1016/0092-8674(92)90172-9
10.1101/gad.7.6.1033
10.1073/pnas.88.22.10004
10.1038/365227a0
10.1126/science.2363050
10.1038/354369a0
10.1016/0092-8674(85)90306-X
10.1016/0092-8674(90)90683-6
10.1101/gad.5.11.1935
10.1016/S0021-9258(18)55000-6 / J. Biol. Chem. / The yeast general transcription factor TFIIA is composed of two polypeptide subunits. (1991)
10.1126/science.1546313
{'key': '2021111418110922000_7.11.2235.26', 'first-page': '3322', 'article-title': 'Factors involved in specific transcription by mammalian RNA polymerase II.', 'volume': '262', 'year': '1987', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Factors involved in specific transcription by mammalian RNA polymerase II. (1987)
10.1016/0378-1119(87)90165-X
10.1038/362175a0
10.1016/S0021-9258(19)45397-0 / J. Biol. Chem. / Separation and characterization of factors mediating accurate transcription by RNA polymerase II. (1982)
10.1073/pnas.82.13.4394
10.1101/gad.5.12a.2212
10.1126/science.3413495
10.1016/S0021-9258(17)45349-X / J. Biol. Chem. / Fractionation of the general RNA polymerase II transcription factors from Drosophila embryos. (1990)
10.1038/362511a0
10.1016/0092-8674(93)90286-Y
10.1016/0955-0674(92)90016-6
10.1101/gad.6.10.1964

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:15 p.m.)
Indexed	2 weeks, 3 days ago (Aug. 5, 2025, 9:09 a.m.)
Issued	31 years, 9 months ago (Nov. 1, 1993)
Published	31 years, 9 months ago (Nov. 1, 1993)
Published Online	31 years, 9 months ago (Nov. 1, 1993)
Published Print	31 years, 9 months ago (Nov. 1, 1993)

Funders 0

None

BibTeX

@article{Yokomori_1993, title={Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.11.2235}, DOI={10.1101/gad.7.11.2235}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Yokomori, K and Admon, A and Goodrich, J A and Chen, J L and Tjian, R}, year={1993}, month=nov, pages={2235–2245} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T13:09:26Z",
    "timestamp": 1754399366353
  },
  "reference-count": 37,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "11",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1993,
        11
      ]
    ]
  },
  "abstract": "<jats:p>The basal factor TFIIA has been shown to act early during initiation in both the mammalian and yeast transcription systems, but a TFIIA-like activity has not been identified in Drosophila. While characterizing the Drosophila TFIID complex, we discovered that a 30-kD protein that cofractionated with dTFIID was homologous to the previously identified, large subunit of yeast TFIIA. Here, we report the cloning and biochemical characterization of Drosophila TFIIA-L. Coimmunoprecipitation studies with anti-dTBP, anti-dTFIIA-L, and anti-TAF antibodies indicated a tight association of the endogenous dTFIIA and dTFIID. However, dTFIIA could be dissociated from dTFIID under conditions that did not elute the TAFs, and the eluted material had mobility shift and transcriptional activities associated with TFIIA. Peptide sequence and Western analysis with antibodies raised against the amino- and carboxy-terminal portions of recombinant dTFIIA-L revealed that a precursor 48-kD species was cleaved in vivo, giving rise to the 30- and 20-kD subunits of dTFIIA that remain associated with each other and with dTFIID. Protein-protein interaction assays identified dTBP and dTAFII110 as targets for binding TFIIA in the TFIID complex. These results suggest that TFIIA may form a specific complex with both TAFs and other components of the transcriptional machinery during formation of the initiation complex.</jats:p>",
  "DOI": "10.1101/gad.7.11.2235",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        6,
        5
      ]
    ],
    "date-time": "2007-06-05T21:15:46Z",
    "timestamp": 1181078146000
  },
  "page": "2235-2245",
  "source": "Crossref",
  "is-referenced-by-count": 91,
  "title": "Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex.",
  "prefix": "10.1101",
  "volume": "7",
  "author": [
    {
      "given": "K",
      "family": "Yokomori",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "A",
      "family": "Admon",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J A",
      "family": "Goodrich",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J L",
      "family": "Chen",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "R",
      "family": "Tjian",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        1993,
        11,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111418110922000_7.11.2235.1",
      "first-page": "461",
      "article-title": "Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF.",
      "volume": "8",
      "year": "1992",
      "journal-title": "Nature"
    },
    {
      "key": "2021111418110922000_7.11.2235.2",
      "first-page": "1602",
      "article-title": "RAP30/RAP74: A general initiation factor that binds to RNA polymerase II.",
      "volume": "8",
      "year": "1988",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111418110922000_7.11.2235.3",
      "first-page": "413",
      "article-title": "Factors involved in transcription by mammalian RNA polymerase II: Purification and analysis of transcription factor IIA and identification of transcription factor IIJ.",
      "volume": "12",
      "year": "1992",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111418110922000_7.11.2235.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.3.11.1677"
    },
    {
      "key": "2021111418110922000_7.11.2235.5",
      "first-page": "563",
      "article-title": "Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation.",
      "volume": "55",
      "year": "1991",
      "journal-title": "Cell"
    },
    {
      "key": "2021111418110922000_7.11.2235.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/363176a0"
    },
    {
      "key": "2021111418110922000_7.11.2235.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/355464a0"
    },
    {
      "key": "2021111418110922000_7.11.2235.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1529339"
    },
    {
      "key": "2021111418110922000_7.11.2235.9",
      "doi-asserted-by": "crossref",
      "first-page": "2786",
      "DOI": "10.1016/S0021-9258(18)45947-9",
      "article-title": "Factors involved in specific transcription initiation by RNA polymerase II: Identification and characterization of factor IIH.",
      "volume": "267",
      "year": "1992",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418110922000_7.11.2235.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/352689a0"
    },
    {
      "key": "2021111418110922000_7.11.2235.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/362179a0"
    },
    {
      "key": "2021111418110922000_7.11.2235.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90682-5"
    },
    {
      "key": "2021111418110922000_7.11.2235.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90664-C"
    },
    {
      "key": "2021111418110922000_7.11.2235.14",
      "doi-asserted-by": "crossref",
      "first-page": "9304",
      "DOI": "10.1016/S0021-9258(18)31585-0",
      "article-title": "Factors involved in specific transcription by mammalian RNA polymerase II: Purification and functional analysis of general transcription factor IIE.",
      "volume": "266",
      "year": "1991",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418110922000_7.11.2235.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90172-9"
    },
    {
      "key": "2021111418110922000_7.11.2235.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.7.6.1033"
    },
    {
      "key": "2021111418110922000_7.11.2235.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.88.22.10004"
    },
    {
      "key": "2021111418110922000_7.11.2235.18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/365227a0"
    },
    {
      "key": "2021111418110922000_7.11.2235.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.2363050"
    },
    {
      "key": "2021111418110922000_7.11.2235.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/354369a0"
    },
    {
      "key": "2021111418110922000_7.11.2235.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(85)90306-X"
    },
    {
      "key": "2021111418110922000_7.11.2235.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90683-6"
    },
    {
      "key": "2021111418110922000_7.11.2235.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.5.11.1935"
    },
    {
      "key": "2021111418110922000_7.11.2235.24",
      "doi-asserted-by": "crossref",
      "first-page": "19320",
      "DOI": "10.1016/S0021-9258(18)55000-6",
      "article-title": "The yeast general transcription factor TFIIA is composed of two polypeptide subunits.",
      "volume": "266",
      "year": "1991",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418110922000_7.11.2235.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1546313"
    },
    {
      "key": "2021111418110922000_7.11.2235.26",
      "first-page": "3322",
      "article-title": "Factors involved in specific transcription by mammalian RNA polymerase II.",
      "volume": "262",
      "year": "1987",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418110922000_7.11.2235.27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(87)90165-X"
    },
    {
      "key": "2021111418110922000_7.11.2235.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/362175a0"
    },
    {
      "key": "2021111418110922000_7.11.2235.29",
      "doi-asserted-by": "crossref",
      "first-page": "14419",
      "DOI": "10.1016/S0021-9258(19)45397-0",
      "article-title": "Separation and characterization of factors mediating accurate transcription by RNA polymerase II.",
      "volume": "257",
      "year": "1982",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418110922000_7.11.2235.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.82.13.4394"
    },
    {
      "key": "2021111418110922000_7.11.2235.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.5.12a.2212"
    },
    {
      "key": "2021111418110922000_7.11.2235.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3413495"
    },
    {
      "key": "2021111418110922000_7.11.2235.33",
      "doi-asserted-by": "crossref",
      "first-page": "21223",
      "DOI": "10.1016/S0021-9258(17)45349-X",
      "article-title": "Fractionation of the general RNA polymerase II transcription factors from Drosophila embryos.",
      "volume": "265",
      "year": "1990",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111418110922000_7.11.2235.34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/362511a0"
    },
    {
      "key": "2021111418110922000_7.11.2235.35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90286-Y"
    },
    {
      "key": "2021111418110922000_7.11.2235.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0955-0674(92)90016-6"
    },
    {
      "key": "2021111418110922000_7.11.2235.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.10.1964"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.7.11.2235",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        15
      ]
    ],
    "date-time": "2021-11-15T02:15:14Z",
    "timestamp": 1636942514000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.7.11.2235"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1993,
        11
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "11",
    "published-online": {
      "date-parts": [
        [
          1993,
          11,
          1
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          1993,
          11
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.7.11.2235"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.7.11.2235",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1993,
        11
      ]
    ]
  }
}