A single cDNA, hTFIIA/alpha, encodes both the p35 and p19 subunits of human TFIIA.
10.1101/gad.7.11.2220
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

TFIIA is a transcription factor that, by interacting with the TATA-binding subunit (TBP) of TFIID, modulates transcription initiation by RNA polymerase II in vitro. By use of a mobility shift assay, TFIIA was purified from HeLa cells as a complex of 35-, 19-, and 12-kD subunits. Oligonucleotides were used to isolate a human cDNA clone, hTFIIA/alpha, which encodes a 55-kD protein with homology to the product of the yeast gene TOA1. The open reading frame of hTFIIA/alpha contains peptide sequences obtained from both the p35 and p19 subunits of natural human TFIIA, and thus encodes these two subunits. Consistent with this, antiserum raised against the 55-kD hTFIIA/alpha-encoded protein reacted with both the p35 and p19 subunits of natural TFIIA, and the recombinant protein could functionally replace those subunits in a mobility shift assay with renatured p12. An efficient affinity purification for natural human TFIIA was suggested by the sequence of the hTFIIA/alpha protein and demonstrated biochemically. Finally, transcription from the adenovirus major late promoter was greatly reduced in nuclear extracts depleted with anti-TFIIA/alpha serum and was restored to original levels by the readdition of purified human TFIIA.

Bibliography

DeJong, J., & Roeder, R. G. (1993). A single cDNA, hTFIIA/alpha, encodes both the p35 and p19 subunits of human TFIIA. Genes & Development, 7(11), 2220–2234.

Authors 2
  1. J DeJong (first)
  2. R G Roeder (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:15 p.m.)
Indexed 3 weeks, 6 days ago (Aug. 5, 2025, 9:07 a.m.)
Issued 31 years, 10 months ago (Nov. 1, 1993)
Published 31 years, 10 months ago (Nov. 1, 1993)
Published Online 31 years, 10 months ago (Nov. 1, 1993)
Published Print 31 years, 10 months ago (Nov. 1, 1993)
Funders 0

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@article{DeJong_1993, title={A single cDNA, hTFIIA/alpha, encodes both the p35 and p19 subunits of human TFIIA.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.11.2220}, DOI={10.1101/gad.7.11.2220}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={DeJong, J and Roeder, R G}, year={1993}, month=nov, pages={2220–2234} }