DOI: 10.1101/gad.7.11.2149. Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast.

Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast.

10.1101/gad.7.11.2149

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

MCM2 and MCM3 are two genetically interacting and structurally related proteins essential for growth in Saccharomyces cerevisiae. Mutants defective in these proteins affect the stability of minichromosomes in general, but the severity of the defect is dependent on the autonomously replicating sequence (ARS) that drives the replication of that plasmid. In this paper we show by two-dimensional gel electrophoresis that the initiation of DNA synthesis at chromosomal replication origins is also reduced in frequency in these mutants. We show further that the nuclear and subnuclear localizations of the MCM2 and MCM3 proteins are temporally regulated with respect to the cell cycle. These proteins enter the nucleus at the end of mitosis, persist there throughout G1 phase, and disappear from it at the beginning of S phase. Once inside the nucleus, a fraction of the MCM2 and MCM3 proteins becomes tightly associated with DNA. The association of MCM2 and MCM3 with chromatin presumably leads to the initiation of DNA synthesis, and their subsequent disappearance from the nucleus presumably prevents reinitiation of DNA synthesis at replication origins. This temporally and spatially restricted localization of MCM2 and MCM3 in the nucleus may serve to ensure that DNA replication occurs once and only once per cell cycle.

Bibliography

Yan, H., Merchant, A. M., & Tye, B. K. (1993). Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast. Genes & Development, 7(11), 2149â2160.

Authors 3

H Yan (first)
A M Merchant (additional)
B K Tye (additional)

References 39 Referenced 166

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Gibson, S.I. 1989. “Analysis of mcm3, a minichromosome maintenance mutant of yeast with a cell division cycle arrest phenotype.” Ph.D. thesis, Cornell University, Ithaca, NY.
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Harlow, E. and D. Lane. 1988. Antibodies: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
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Pringle, J.R. and L.H. Hartwell. 1981. The Saccharomyces cerevisiae cell cycle. In The molecular biology of the yeast Saccharomyces J.N. Strathern, E.W. Jones, and J.R. Broach, pp. 79–142. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 14, 2021, 9:14 p.m.)
Indexed	1 year, 5 months ago (March 20, 2024, 9:17 p.m.)
Issued	31 years, 9 months ago (Nov. 1, 1993)
Published	31 years, 9 months ago (Nov. 1, 1993)
Published Online	31 years, 9 months ago (Nov. 1, 1993)
Published Print	31 years, 9 months ago (Nov. 1, 1993)

Funders 0

None

BibTeX

@article{Yan_1993, title={Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.11.2149}, DOI={10.1101/gad.7.11.2149}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Yan, H and Merchant, A M and Tye, B K}, year={1993}, month=nov, pages={2149–2160} }

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