A switch from Myc:Max to Mad:Max heterocomplexes accompanies monocyte/macrophage differentiation.
10.1101/gad.7.11.2110
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

Mad is a basic-helix-loop-helix-zipper protein that heterodimerizes with Max in vitro. Mad:Max heterodimers recognize the same E-box-related DNA-binding sites as Myc:Max heterodimers. However, in transient transfection assays Myc and Mad influence transcription in opposite ways through interaction with Max; Myc activates while Mad represses transcription. Here, we demonstrate that Mad protein is induced rapidly upon differentiation of cells of the myeloid lineage. The Mad protein is synthesized in human cells as a 35-kD nuclear phosphoprotein with an extremely short half-life (t1/2 = 15-30 min) and can be detected in vivo in a complex with Max. In the undifferentiated U937 monocyte cell line Max was found complexed with Myc but not Mad. However, Mad:Max complexes began to accumulate as early as 2 hr after induction of macrophage differentiation with TPA. By 48 hr following TPA treatment only Mad:Max complexes were detectable. These data show that differentiation is accompanied by a change in the composition of Max heterocomplexes. We speculate that this switch in heterocomplexes results in a change in the transcriptional regulation of Myc:Max target genes required for cell proliferation.

Bibliography

Ayer, D. E., & Eisenman, R. N. (1993). A switch from Myc:Max to Mad:Max heterocomplexes accompanies monocyte/macrophage differentiation. Genes & Development, 7(11), 2110–2119.

Authors 2
  1. D E Ayer (first)
  2. R N Eisenman (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 14, 2021, 9:14 p.m.)
Indexed 2 months ago (June 21, 2025, 12:10 a.m.)
Issued 31 years, 9 months ago (Nov. 1, 1993)
Published 31 years, 9 months ago (Nov. 1, 1993)
Published Online 31 years, 9 months ago (Nov. 1, 1993)
Published Print 31 years, 9 months ago (Nov. 1, 1993)
Funders 0

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@article{Ayer_1993, title={A switch from Myc:Max to Mad:Max heterocomplexes accompanies monocyte/macrophage differentiation.}, volume={7}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.7.11.2110}, DOI={10.1101/gad.7.11.2110}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Ayer, D E and Eisenman, R N}, year={1993}, month=nov, pages={2110–2119} }