DOI: 10.1101/gad.6.8.1518. dorsal-twist interactions establish snail expression in the presumptive mesoderm of the Drosophila embryo.

dorsal-twist interactions establish snail expression in the presumptive mesoderm of the Drosophila embryo.

10.1101/gad.6.8.1518

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The first step in the differentiation of the Drosophila mesoderm is the activation of two regulatory genes, twist (twi) and snail (sna), in ventral regions of early embryos. sna is a transcriptional repressor that is uniformly expressed throughout the presumptive mesoderm. Its sharp lateral limits help to establish the boundary between the mesoderm and neuroectoderm. Genetic studies suggest that sna is a target of the dorsal (dl) morphogen, and this interaction provides a model for determining how a morphogen gradient establishes a sharp, on/off threshold response. We present evidence that dl and twi directly activate sna expression. Site-directed mutagenesis of dl- and twi-binding sites within defined regions of the sna promoter suggest that the two proteins (containing the Rel and helix-loop-helix domains, respectively) function multiplicatively to ensure strong, uniform expression of sna, particularly in ventral-lateral regions where there are diminishing amounts of dl. These results are consistent with the possibility that the sharp sna borders are formed by multiplying the shallow dl gradient and the steeper twi gradient.

Bibliography

Ip, Y. T., Park, R. E., Kosman, D., Yazdanbakhsh, K., & Levine, M. (1992). dorsal-twist interactions establish snail expression in the presumptive mesoderm of the Drosophila embryo. Genes & Development, 6(8), 1518â1530.

Authors 5

Y T Ip (first)
R E Park (additional)
D Kosman (additional)
K Yazdanbakhsh (additional)
M Levine (additional)

References 44 Referenced 262

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{'key': '2021111320240783000_6.8.1518.5', 'first-page': '1515', 'article-title': 'Spatial regulation of zerknüllt: A dorsal-ventral patterning gene in Drosophila.', 'volume': '3', 'year': '1989', 'journal-title': 'Genes & Dev.'} / Genes & Dev. / Spatial regulation of zerknüllt: A dorsal-ventral patterning gene in Drosophila. (1989)
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{'key': '2021111320240783000_6.8.1518.13', 'first-page': '15', 'article-title': 'The role of the dorsal morphogen gradient in Drosophila embryogenesis.', 'volume': '3', 'year': '1992', 'journal-title': 'Sem. Dev. Biol.'} / Sem. Dev. Biol. / The role of the dorsal morphogen gradient in Drosophila embryogenesis. (1992)
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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:27 p.m.)
Indexed	2 months, 2 weeks ago (June 11, 2025, 10:43 p.m.)
Issued	33 years ago (Aug. 1, 1992)
Published	33 years ago (Aug. 1, 1992)
Published Online	33 years ago (Aug. 1, 1992)
Published Print	33 years ago (Aug. 1, 1992)

Funders 0

None

BibTeX

@article{Ip_1992, title={dorsal-twist interactions establish snail expression in the presumptive mesoderm of the Drosophila embryo.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.8.1518}, DOI={10.1101/gad.6.8.1518}, number={8}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Ip, Y T and Park, R E and Kosman, D and Yazdanbakhsh, K and Levine, M}, year={1992}, month=aug, pages={1518–1530} }

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