DOI: 10.1101/gad.6.8.1480. Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.

Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.

10.1101/gad.6.8.1480

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The myc family of cellular oncogenes encodes three highly related nuclear phosphoproteins (c-Myc, N-Myc, and L-Myc) that are believed to function as sequence-specific transcription factors capable of regulating genes important in cellular growth and differentiation. Current evidence indicates that Myc family proteins exist as biologically active heterodimeric complexes in association with another helix-loop-helix leucine zipper phosphoprotein, Max. We have investigated the common and unique properties among the Myc family, as well as the physiological role of Max in the regulation of Myc family function. We demonstrate that trans-activation-incompetent mutants of one Myc family member can act in trans to dominantly suppress the cotransformation activities of all three Myc oncoproteins, indicating that the Myc family functions through common genetic elements in its cellular transformation pathways. Employing co-immunoprecipitation with either anti-Myc or anti-Max antibodies, we show that the transfected normal c-Myc, N-Myc, and L-Myc oncoproteins associate with the endogenous Max protein in REF transformants, indicating that the Max interaction represents at least one component common to Myc family function. In addition, we observed a striking reduction in Myc cotransformation activity when a Max expression construct was added to myc/ras co-transfections. We discuss these biological findings in the context of a proposed model for Myc/Max function and regulation in which Max serves as either an obligate partner in the Myc/Max transcriptional complex or as a repressor in the form of a transcriptionally inert Max/Max homodimer capable of occupying Myc/Max-responsive gene targets.

Bibliography

Mukherjee, B., Morgenbesser, S. D., & DePinho, R. A. (1992). Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants. Genes & Development, 6(8), 1480â1492.

Authors 3

B Mukherjee (first)
S D Morgenbesser (additional)
R A DePinho (additional)

References 45 Referenced 99

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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	1 year, 6 months ago (Feb. 14, 2024, 4:29 p.m.)
Indexed	1 year ago (Aug. 7, 2024, 8:33 a.m.)
Issued	33 years, 1 month ago (Aug. 1, 1992)
Published	33 years, 1 month ago (Aug. 1, 1992)
Published Online	33 years, 1 month ago (Aug. 1, 1992)
Published Print	33 years, 1 month ago (Aug. 1, 1992)

Funders 0

None

BibTeX

@article{Mukherjee_1992, title={Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.8.1480}, DOI={10.1101/gad.6.8.1480}, number={8}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Mukherjee, B and Morgenbesser, S D and DePinho, R A}, year={1992}, month=aug, pages={1480–1492} }

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