A ubiquitous factor is required for C/EBP-related proteins to form stable transcription complexes on an albumin promoter segment in vitro.
10.1101/gad.6.6.991
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The liver-enriched transcription factor CCAAT/enhancer binding protein (C/EBP) binds to numerous liver-specific promoters, yet the mechanism by which the protein stimulates transcription has not been described. The serum albumin promoter, which is liver specific, contains a strong C/EBP-binding site tightly juxtaposed to a binding site for the ubiquitous factor nuclear factor-Y (NF-Y). The binding of C/EBP impairs the binding of NF-Y; yet surprisingly, this arrangement leads to strong synergistic activation of a minimal promoter in liver nuclear extracts. Transcriptional synergism is manifested by NF-Y facilitating the ability of C/EBP to form preinitiation complexes that are stable through multiple rounds of transcription. Binding by itself, C/EBP stimulates transcription weakly without forming stable complexes, and moving the NF-Y binding site 10 bp away from the C/EBP site increases NF-Y binding in the presence of C/EBP but reduces the efficiency of stable complex formation and transcriptional synergism. These findings show that C/EBP requires precise positioning next to a ubiquitous factor for optimal formation of stable complexes and provides a model to understand the dramatic activation of the albumin gene in hepatic development.

Bibliography

Milos, P. M., & Zaret, K. S. (1992). A ubiquitous factor is required for C/EBP-related proteins to form stable transcription complexes on an albumin promoter segment in vitro. Genes & Development, 6(6), 991–1004.

Authors 2
  1. P M Milos (first)
  2. K S Zaret (additional)
References 55 Referenced 102
  1. 10.1101/gad.1.3.256
  2. 10.1101/gad.3.8.1146
  3. 10.1073/pnas.84.19.6673
  4. 10.1101/gad.5.9.1538
  5. 10.1038/345361a0
  6. 10.1242/dev.113.1.217 / Development / Hepatocyte differentiation initiated during endodermal-mesenchymal interaction prior to liver formation. (1991)
  7. 10.1016/0092-8674(87)90036-5
  8. 10.1101/gad.2.8.957
  9. 10.1073/pnas.85.21.7937
  10. 10.1126/science.1899487
  11. 10.1016/0092-8674(91)90531-3
  12. 10.1101/gad.4.9.1541
  13. 10.1126/science.2943018
  14. 10.1016/0092-8674(89)90877-5
  15. 10.1101/gad.4.8.1416
  16. 10.1016/0092-8674(88)90109-2
  17. 10.1016/0092-8674(86)90519-2
  18. 10.1016/0092-8674(88)90119-5
  19. 10.1016/S0021-9258(17)39577-7 / J. Biol. Chem. / Separation and partial characterization of three functional steps in transcription initiation by human RNA polymerase II. (1985)
  20. 10.1016/S0021-9258(18)61372-9 / J. Biol. Chem. / Functional steps ininitiation and reinitiation from the major late promoter in a HeLa nuclear extract. (1987)
  21. {'key': '2021111320224972000_6.6.991.21', 'first-page': '4750', 'article-title': 'The rat albumin promoter is composed of six distinct positive elements within 130 nucleotides.', 'volume': '9', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The rat albumin promoter is composed of six distinct positive elements within 130 nucleotides. (1989)
  22. 10.1073/pnas.86.5.1553
  23. 10.1002/j.1460-2075.1990.tb07509.x / EMBO J. / Co-evolution from yeast to mouse: cDNA cloning of the two NF-Y (CP-1/CBF) subunits. (1990)
  24. 10.1016/S0092-8674(88)80011-4
  25. 10.1016/0092-8674(88)90118-3
  26. 10.1101/gad.1.2.133
  27. 10.1073/pnas.83.16.5889
  28. 10.1126/science.3289117
  29. 10.1016/0092-8674(87)90583-6
  30. 10.1016/0092-8674(91)90321-O
  31. 10.1038/345359a0
  32. 10.1101/gad.2.5.528
  33. {'key': '2021111320224972000_6.6.991.33', 'first-page': '773', 'article-title': 'Extracellular signals that regulate liver transcription factors during hepatic differentation in vitro.', 'volume': '11', 'year': '1991', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Extracellular signals that regulate liver transcription factors during hepatic differentation in vitro. (1991)
  34. 10.1126/science.2711183
  35. 10.1073/pnas.87.14.5378
  36. 10.1016/0092-8674(90)90808-R
  37. 10.1073/pnas.87.10.3992
  38. 10.1101/gad.4.10.1714
  39. 10.1073/pnas.88.1.224
  40. 10.1002/j.1460-2075.1987.tb04854.x / EMBO J. / Discrete elements within the SV40 enhancer region display different cell-specific enhancer activities. (1987)
  41. 10.1038/333040a0
  42. 10.1002/j.1460-2075.1984.tb02164.x / EMBO J. / Tissue-specific expression is conferred by a sequence from the 5′ end of the rat albumin gene. (1984)
  43. 10.1101/gad.1.10.1172
  44. 10.1101/gad.1.3.268
  45. 10.1016/0092-8674(90)90459-R
  46. 10.1073/pnas.85.3.757
  47. 10.1016/S0021-9258(18)61506-6 / J. Biol. Chem. / Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site. (1987)
  48. 10.1146/annurev.bi.59.070190.003431
  49. 10.1101/gad.1.1.65
  50. 10.1038/319121a0
  51. 10.1073/pnas.79.17.5254
  52. 10.1126/science.2683088
  53. 10.1101/gad.5.9.1553
  54. 10.1016/0022-2836(90)90341-I
  55. 10.1073/pnas.85.23.9076
Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:24 p.m.)
Indexed 3 months, 1 week ago (May 22, 2025, 5:30 a.m.)
Issued 33 years, 2 months ago (June 1, 1992)
Published 33 years, 2 months ago (June 1, 1992)
Published Online 33 years, 2 months ago (June 1, 1992)
Published Print 33 years, 2 months ago (June 1, 1992)
Funders 0

None

@article{Milos_1992, title={A ubiquitous factor is required for C/EBP-related proteins to form stable transcription complexes on an albumin promoter segment in vitro.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.6.991}, DOI={10.1101/gad.6.6.991}, number={6}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Milos, P M and Zaret, K S}, year={1992}, month=jun, pages={991–1004} }