A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation.
10.1101/gad.6.5.801
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The yeast RAP1 protein is a sequence-specific DNA-binding protein that functions as both a repressor and an activator of transcription. RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly(C1-3A) sequences. Previous studies have indicated that the regulatory function of RAP1 is determined by the context of its binding site and, presumably, its interactions with other factors. Using the two-hybrid system, a genetic screen for the identification of protein-protein interactions, we have isolated a gene encoding a RAP1-interacting factor (RIF1). Strains carrying gene disruptions of RIF1 grow normally but are defective in transcriptional silencing and telomere length regulation, two phenotypes strikingly similar to those of silencing-defective rap1s mutants. Furthermore, hybrid proteins containing rap1s missense mutations are defective in an interaction with RIF1 in the two-hybrid system. Taken together, these data support the idea that the rap1s phenotypes are attributable to a failure to recruit RIF1 to silencers and telomeres and suggest that RIF1 is a cofactor or mediator for RAP1 in the establishment of a repressed chromatin state at these loci. By use of the two-hybrid system, we have isolated a mutation in RIF1 that partially restores the interaction with rap1s mutant proteins.

Bibliography

Hardy, C. F., Sussel, L., & Shore, D. (1992). A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation. Genes & Development, 6(5), 801–814.

Authors 3
  1. C F Hardy (first)
  2. L Sussel (additional)
  3. D Shore (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:25 p.m.)
Indexed 1 week, 1 day ago (Aug. 19, 2025, 5:56 a.m.)
Issued 33 years, 3 months ago (May 1, 1992)
Published 33 years, 3 months ago (May 1, 1992)
Published Online 33 years, 3 months ago (May 1, 1992)
Published Print 33 years, 3 months ago (May 1, 1992)
Funders 0

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@article{Hardy_1992, title={A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.5.801}, DOI={10.1101/gad.6.5.801}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Hardy, C F and Sussel, L and Shore, D}, year={1992}, month=may, pages={801–814} }