Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes.
10.1101/gad.6.4.533
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

Previous studies suggest that the CCAAT/enhancer-binding protein (C/EBP) functions in the coordinate expression of adipocyte genes during differentiation of 3T3-L1 preadipocytes. We sought to block expression of C/EBP selectively using a bovine papilloma virus (BPV) vector to direct transcription of a approximately 0.4-kb segment of C/EBP cDNA (in antisense orientation) containing translated sequence 5' to that encoding the basic and leucine zipper regions of the protein. Vector-directed expression of antisense C/EBP RNA in 3T3-L1 preadipocytes inhibited expression of C/EBP mRNA and protein, as well as several adipose-specific mRNAs, and also prevented cytoplasmic triglyceride accumulation. Rescue of the "adipocyte phenotype" was accomplished by transfection of cells expressing antisense RNA with a modified BPV vector that directs transcription of the complementary sense C/EBP RNA.

Bibliography

Lin, F. T., & Lane, M. D. (1992). Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes. Genes & Development, 6(4), 533–544.

Authors 2
  1. F T Lin (first)
  2. M D Lane (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:25 p.m.)
Indexed 57 minutes ago (Aug. 30, 2025, 12:20 p.m.)
Issued 33 years, 4 months ago (April 1, 1992)
Published 33 years, 4 months ago (April 1, 1992)
Published Online 33 years, 4 months ago (April 1, 1992)
Published Print 33 years, 4 months ago (April 1, 1992)
Funders 0

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@article{Lin_1992, title={Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.4.533}, DOI={10.1101/gad.6.4.533}, number={4}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Lin, F T and Lane, M D}, year={1992}, month=apr, pages={533–544} }