A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements.
10.1101/gad.6.3.466
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

We have identified a Drosophila transcription factor that binds to fat body-specific enhancers of alcohol dehydrogenase (Adh) and yolk protein genes. DNA sequence analysis of cDNA clones encoding this protein, box B-binding factor-2 (BBF-2), indicates that it is a member of the CREB/ATF family of transcriptional regulatory proteins. A number of observations suggest that BBF-2 is involved in fat body-specific expression: Mutations that disrupt BBF-2 binding to two different Adh fat body enhancers in vitro decrease the activity of these enhancers in transgenic flies. BBF-2 mRNA is present in all cell types examined, and the protein is present in cells that express ADH. Finally, BBF-2 is a transcriptional activator in Drosophila tissue culture cells. Remarkably, BBF-2 also binds specifically to regulatory elements required for liver-specific expression of the human Adh and rat tyrosine aminotransferase genes. Thus, BBF-2 and the DNA sequence to which it binds may be important components of a tissue-specific regulatory mechanism conserved between Drosophila and man.

Bibliography

Abel, T., Bhatt, R., & Maniatis, T. (1992). A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements. Genes & Development, 6(3), 466–480.

Authors 3
  1. T Abel (first)
  2. R Bhatt (additional)
  3. T Maniatis (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:25 p.m.)
Indexed 1 year, 2 months ago (June 24, 2024, 3:55 p.m.)
Issued 33 years, 5 months ago (March 1, 1992)
Published 33 years, 5 months ago (March 1, 1992)
Published Online 33 years, 5 months ago (March 1, 1992)
Published Print 33 years, 5 months ago (March 1, 1992)
Funders 0

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@article{Abel_1992, title={A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.3.466}, DOI={10.1101/gad.6.3.466}, number={3}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Abel, T and Bhatt, R and Maniatis, T}, year={1992}, month=mar, pages={466–480} }