TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters.
10.1101/gad.6.2.304
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

We have studied the effect of exogenous expression of the basal transcription factor TFIID on the activities of several different TATA-containing and TATA-lacking promoters. Overexpression of TFIID from a transfected plasmid in Drosophila Schneider cells resulted in substantial concentration-dependent increases in expression from a cotransfected minimal TATA-containing promoter. Overexpression of TFIID activated expression from all TATA-containing promoters tested, with the maximum level of activation being inversely proportional to the strength of the promoter. In contrast, expression from TATA-less promoters was not enhanced, and could in fact be reduced, by increased expression of TFIID. Consistent with these findings overexpression of TFIID had opposite effects on Sp1-mediated activation observed from minimal synthetic promoters consisting of Sp1-binding sites and either a TATA box or initiator element. We discuss the significance of these results in terms of the role of TFIID in the initiation of transcription and as a possible regulatory target for expression from TATA-containing promoters, as well as the role TFIID may play in expression from TATA-less promoters.

Bibliography

Colgan, J., & Manley, J. L. (1992). TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters. Genes & Development, 6(2), 304–315.

Authors 2
  1. J Colgan (first)
  2. J L Manley (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:23 p.m.)
Indexed 2 weeks, 6 days ago (Aug. 5, 2025, 9:12 a.m.)
Issued 33 years, 6 months ago (Feb. 1, 1992)
Published 33 years, 6 months ago (Feb. 1, 1992)
Published Online 33 years, 6 months ago (Feb. 1, 1992)
Published Print 33 years, 6 months ago (Feb. 1, 1992)
Funders 0

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@article{Colgan_1992, title={TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.2.304}, DOI={10.1101/gad.6.2.304}, number={2}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Colgan, J and Manley, J L}, year={1992}, month=feb, pages={304–315} }