DOI: 10.1101/gad.6.11.2214. Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.

Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.

10.1101/gad.6.11.2214

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The Escherichia coli ruvA and ruvB genes are involved in DNA repair and in the late step of homologous genetic recombination. We have demonstrated previously that the RuvA-RuvB protein complex in the presence of ATP promotes reabsorption of cruciform structures extruded from a supercoiled plasmid with an inverted repeat sequence. Because the cruciform structure is topologically analogous to the Holiday structure, we have proposed that the role of the RuvA and RuvB proteins in recombination is to promote a strand exchange reaction at the Holliday junction. Here, we studied the specific interaction of the RuvA-RuvB complex with the Holliday structure using synthetic analogs prepared by annealing four oligonucleotides. The affinities of the RuvA protein for synthetic Holliday junctions are much higher (> 20-fold) than for duplex DNA, and the affinities of the RuvA protein for the junctions are further enhanced (> 4-fold) by the interaction with the RuvB protein. The RuvA-RuvB protein complex in the presence of ATP promotes dissociation of the synthetic Holliday junction with homology in the central core into two halves by catalyzing branch migration to the DNA ends, but it does not affect the structure of the synthetic Holliday junction without the homology. The separation of the synthetic Holliday junction is a result of the activity of the RuvA-RuvB complex that promotes strand exchange and DNA unwinding. Furthermore, RuvA and RuvB promote the strand exchange reaction at the Holliday junctions made by RecA. These results provide further evidence that the RuvA-RuvB complex recognizes the Holliday junction and promotes branch migration in homologous recombination.

Bibliography

Iwasaki, H., Takahagi, M., Nakata, A., & Shinagawa, H. (1992). Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes & Development, 6(11), 2214â2220.

Authors 4

H Iwasaki (first)
M Takahagi (additional)
A Nakata (additional)
H Shinagawa (additional)

References 27 Referenced 134

10.1093/nar/16.4.1541
10.1007/BF00269858
10.1038/354506a0
10.1017/S0016672300001233
10.1007/BF00334372
10.1128/jb.171.10.5276-5280.1989 / J. Bacteriol. / Overproduction, purification, and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair. (1989)
10.1002/j.1460-2075.1991.tb05016.x / EMBO J. / Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. (1991)
10.1128/jb.173.17.5414-5418.1991 / J. Bacteriol. / Conjugational recombination in resolvase-deficient ruvC mutants of Escherichia coli K-12 depends on recG. (1991)
10.1128/jb.173.21.6837-6843.1991 / J. Bacteriol. / Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12. (1991)
10.1007/BF00383532
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10.1016/0092-8674(90)90747-3
10.1128/jb.138.1.1-6.1979 / J. Bacteriol. / Thermoresistant revertants of an Escherichia coli strain carrying tif-1 and ruv mutations: Non-suppressibility of ruv by sfi. (1979)
10.1128/jb.117.2.337-344.1974 / J. Bacteriol. / Isolation and characterization of an Escherichia coli ruv mutant which forms nonseptate filaments after low doses of ultraviolet light irradiation. (1974)
10.1016/S0021-9258(19)38845-3 / J. Biol. Chem. / Interaction of a four-way junction in DNA with T4 endonuclease VII. (1990)
10.1073/pnas.89.12.5452
Radding, CM. 1988. Homologous pairing and strand exchange promoted by Escherichia coli RecA protein. In Genetic recombination (ed. R.Kucherlapati and G.R. Smith), pp. 193â229. American Society for Microbiology, Washington, D.C.
10.1016/0165-1218(89)90010-4
10.1128/jb.173.23.7711-7715.1991 / J. Bacteriol. / Resolution of Holliday junctions in Escherichia coli: Identification of the ruvC gene product as a 19-kilodalton protein. (1991)
10.1007/BF00261724 / Mol.& Gen. Genet. / Molecular and functional analysis of the ruv region of Escherichia coli K-12 reveals three genes involved in DNA repair and recombination. (1990)
10.1073/pnas.88.19.8445
10.1128/jb.170.9.4322-4329.1988 / J. Bacteriol. / Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination. (1988)
10.1016/0300-9084(91)90120-P / Biochimie / Properties of the Escherichia coli RuvA and RuvB proteins involved in DNA repair, recombination and mutagenesis. (1991)
10.1007/BF00330811
10.1128/jb.173.18.5747-5753.1991 / J. Bacteriol. / Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease. (1991)
10.1016/0092-8674(92)90626-N
10.1016/0092-8674(92)90638-S

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:31 p.m.)
Indexed	1 year ago (Aug. 7, 2024, 8:37 a.m.)
Issued	32 years, 9 months ago (Nov. 1, 1992)
Published	32 years, 9 months ago (Nov. 1, 1992)
Published Online	32 years, 9 months ago (Nov. 1, 1992)
Published Print	32 years, 9 months ago (Nov. 1, 1992)

Funders 0

None

BibTeX

@article{Iwasaki_1992, title={Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.11.2214}, DOI={10.1101/gad.6.11.2214}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Iwasaki, H and Takahagi, M and Nakata, A and Shinagawa, H}, year={1992}, month=nov, pages={2214–2220} }

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