DOI: 10.1101/gad.6.11.2077. The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence.

The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence.

10.1101/gad.6.11.2077

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Among eukaryotic transcription trans-activators, the human immunodeficiency virus type 1 (HIV-1) Tat protein is exceptional in that its target site TAR is an RNA rather than a DNA sequence. Here, we confirm that fusion of Tat to the RNA-binding domain of the HIV-1 Rev protein permits the efficient activation of an HIV-1 long terminal repeat (LTR) promoter in which critical TAR sequences have been replaced by RNA sequences derived from the HIV-1 Rev response element (RRE). An RRE target sequence as small as 13 nucleotides is shown to form an effective in vivo target for Rev binding. More important, a fusion protein consisting of Rev attached to the VP16 transcription activation domain was also observed to efficiently activate the HIV-1 LTR from this nascent RNA target. These data demonstrate that trans-activation of transcription by acidic activation domains does not require a stable interaction with the promoter DNA and suggest that VP16, like Tat, can act on steps subsequent to the formation of the HIV-1 LTR preinitiation complex. The finding that the activation domains of VP16 and Tat are functionally interchangeable raises the possibility that these apparently disparate viral trans-activators may nevertheless act via similar mechanisms.

Bibliography

Tiley, L. S., Madore, S. J., Malim, M. H., & Cullen, B. R. (1992). The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence. Genes & Development, 6(11), 2077â2087.

Authors 4

L S Tiley (first)
S J Madore (additional)
M H Malim (additional)
B R Cullen (additional)

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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:28 p.m.)
Indexed	1 week, 1 day ago (Aug. 27, 2025, 11:51 a.m.)
Issued	32 years, 10 months ago (Nov. 1, 1992)
Published	32 years, 10 months ago (Nov. 1, 1992)
Published Online	32 years, 10 months ago (Nov. 1, 1992)
Published Print	32 years, 10 months ago (Nov. 1, 1992)

Funders 0

None

BibTeX

@article{Tiley_1992, title={The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.11.2077}, DOI={10.1101/gad.6.11.2077}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Tiley, L S and Madore, S J and Malim, M H and Cullen, B R}, year={1992}, month=nov, pages={2077–2087} }

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