DOI: 10.1101/gad.6.1.135. A 5'-terminal stem-loop structure can stabilize mRNA in Escherichia coli.

A 5'-terminal stem-loop structure can stabilize mRNA in Escherichia coli.

10.1101/gad.6.1.135

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The 5'-untranslated region of the long-lived Escherichia coli ompA transcript functions as an mRNA stabilizer capable of prolonging the lifetime in E. coli of a number of heterologous messages to which it is fused. To elucidate the structural basis of differential mRNA stability in bacteria, the domains of the ompA 5'-untranslated region that allow it to protect mRNA from degradation have been identified by mutational analysis. The presence of a stem-loop no more than 2-4 nucleotides from the extreme 5' terminus of this RNA segment is crucial to its stabilizing influence, whereas the sequence of the stem-loop is relatively unimportant. The potential to form a hairpin very close to the 5' end is a feature common to a number of stable prokaryotic messages. Moreover, the lifetime of a normally labile message (bla mRNA) can be prolonged in E. coli by adding a simple hairpin structure at its 5' terminus. Accelerated degradation of ompA mRNA in the absence of a 5'-terminal stem-loop appears to start downstream of the 5' end. We propose that E. coli messages beginning with a single-stranded RNA segment of significant length are preferentially targeted by a degradative ribonuclease that interacts with the mRNA 5' terminus before cleaving internally at one or more distal sites.

Bibliography

Emory, S. A., Bouvet, P., & Belasco, J. G. (1992). A 5â-terminal stem-loop structure can stabilize mRNA in Escherichia coli. Genes & Development, 6(1), 135â148.

Authors 3

S A Emory (first)
P Bouvet (additional)
J G Belasco (additional)

References 44 Referenced 260

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Kennell, D.E. 1986. The instability of messenger RNA in bacteria. In Maximizing gene expression (ed. W.S. Reznikoff and L. Gold), pp. 101â142. Butterworths, Stoneham, Massachusetts. (10.1016/B978-0-409-90027-9.50010-9)
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10.1002/j.1460-2075.1990.tb07460.x / EMBO J. / Cleavages in the 5â² region of the ompA and bla mRNA control stability: Studies with an E. coli mutant altering mRNA stability and a novel endoribonuclease. (1990)
10.1128/jb.173.8.2488-2497.1991 / J. Bacteriol. / Specific endonucleolytic cleavage of the mRNA for ribosomal protein S20 of Escherichia coli requires the product of the ams gene in vivo and in vitro. (1991)
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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:23 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 12, 2025, 6:01 p.m.)
Issued	33 years, 7 months ago (Jan. 1, 1992)
Published	33 years, 7 months ago (Jan. 1, 1992)
Published Online	33 years, 7 months ago (Jan. 1, 1992)
Published Print	33 years, 7 months ago (Jan. 1, 1992)

Funders 0

None

BibTeX

@article{Emory_1992, title={A 5’-terminal stem-loop structure can stabilize mRNA in Escherichia coli.}, volume={6}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.6.1.135}, DOI={10.1101/gad.6.1.135}, number={1}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Emory, S A and Bouvet, P and Belasco, J G}, year={1992}, month=jan, pages={135–148} }

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