DOI: 10.1101/gad.5.9.1684. Functional dissection of the paired segmentation gene in Drosophila embryos.

Functional dissection of the paired segmentation gene in Drosophila embryos.

10.1101/gad.5.9.1684

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

An ectopic expression assay in Drosophila embryos was used to investigate the roles of pair-rule segmentation genes in the spatial regulation of the segment-polarity gene, engrailed (en). It is hypothesized that the regions of overlap in expression of two genes, paired (prd) and even-skipped (eve), define the odd-numbered en expression stripes. Consistent with this combinatorial model, ectopic expression of prd caused these en stripes to be expanded posteriorly. Surprisingly, however, ectopic expression of a prd gene with a deletion of the conserved paired box resulted in loss of these odd-numbered en stripes. This dominant negative effect is a phenocopy of en expression in prd embryos and suggests that the paired box is necessary for normal prd- function. A similar deletion of odd-numbered en stripes was also observed after ectopic expression of a chimeric fushi tarazu (ftz) gene containing a substituted prd gene homeo box; in addition, in these embryos, the even-numbered en stripes were expanded anteriorly, as observed when the unaltered ftz gene is ectopically expressed. These effects suggest that the chimeric protein may have DNA or protein targets of both the normal Ftz and Prd proteins.

Bibliography

Morrissey, D., Askew, D., Raj, L., & Weir, M. (1991). Functional dissection of the paired segmentation gene in Drosophila embryos. Genes & Development, 5(9), 1684â1696.

Authors 4

D Morrissey (first)
D Askew (additional)
L Raj (additional)
M Weir (additional)

References 50 Referenced 41

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{'key': '2021111320192410000_5.9.1684.10', 'first-page': '56', 'article-title': 'Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein.', 'volume': '43', 'year': '1985', 'journal-title': 'Cell'} / Cell / Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein. (1985)
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{'key': '2021111320192410000_5.9.1684.41', 'first-page': '25', 'article-title': 'The structure and function of the homeodomain.', 'volume': '989', 'year': '1989', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta / The structure and function of the homeodomain. (1989)
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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:20 p.m.)
Indexed	12 minutes ago (Sept. 3, 2025, 1:35 a.m.)
Issued	34 years ago (Sept. 1, 1991)
Published	34 years ago (Sept. 1, 1991)
Published Online	34 years ago (Sept. 1, 1991)
Published Print	34 years ago (Sept. 1, 1991)

Funders 0

None

BibTeX

@article{Morrissey_1991, title={Functional dissection of the paired segmentation gene in Drosophila embryos.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.9.1684}, DOI={10.1101/gad.5.9.1684}, number={9}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Morrissey, D and Askew, D and Raj, L and Weir, M}, year={1991}, month=sep, pages={1684–1696} }

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