The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation.
10.1101/gad.5.8.1464
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The product of proto-oncogene Rel associates with a number of cellular proteins. We have studied the effect of one of them, a phosphoprotein of 40 kD (pp40), on the DNA-binding activity of the Rel protein. We demonstrate that purified pp40 not only inhibits the binding of Rel, but also NF-kappa B (p50-p65) heterocomplex to DNA. Additionally, I kappa B beta, but not I kappa B alpha, also prevented the binding of Rel to the kappa B site. I kappa B beta and pp40 are related proteins because (1) they share a number of common tryptic peptides, (2) their inhibitory effect on DNA binding can be abolished by preincubation with pp40-specific antiserum, and (3) labeled I kappa B beta can be immunoprecipitated with pp40 antibodies. pp40 is part of the Rel complex present in the cytoplasm and nuclear extracts of WEHI-231 cells. The activity of pp40 to inhibit the DNA binding of Rel and NF-kappa B is modulated by phosphorylation.

Bibliography

Kerr, L. D., Inoue, J., Davis, N., Link, E., Baeuerle, P. A., Bose, H. R., & Verma, I. M. (1991). The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation. Genes & Development, 5(8), 1464–1476.

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  1. L D Kerr (first)
  2. J Inoue (additional)
  3. N Davis (additional)
  4. E Link (additional)
  5. P A Baeuerle (additional)
  6. H R Bose (additional)
  7. I M Verma (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:20 p.m.)
Indexed 3 months, 1 week ago (May 18, 2025, 12:03 p.m.)
Issued 34 years ago (Aug. 1, 1991)
Published 34 years ago (Aug. 1, 1991)
Published Online 34 years ago (Aug. 1, 1991)
Published Print 34 years ago (Aug. 1, 1991)
Funders 0

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@article{Kerr_1991, title={The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.8.1464}, DOI={10.1101/gad.5.8.1464}, number={8}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Kerr, L D and Inoue, J and Davis, N and Link, E and Baeuerle, P A and Bose, H R and Verma, I M}, year={1991}, month=aug, pages={1464–1476} }