DOI: 10.1101/gad.5.7.1224. Characterization of cDNAs encoding the polypyrimidine tract-binding protein.

Characterization of cDNAs encoding the polypyrimidine tract-binding protein.

10.1101/gad.5.7.1224

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The polypyrimidine tract of mammalian introns is recognized by a 62-kD protein (pPTB). Mutations in the polypyrimidine tract that reduce the binding of pPTB also reduce the efficiency of formation of the pre-spliceosome complex containing U2 snRNP. The PTB protein was purified to homogeneity by affinity chromatography on a matrix containing poly(U), and peptide sequence was used to isolate several cDNAs. Because a variety of cell types express mRNA complementary to these cDNAs, PTB may be a ubiquitous splicing factor. Three classes of cDNAs were identified, on the basis of the presence of additional sequences at an internal position. This variation in sequence probably reflects alternative splicing of the PTB pre-mRNA and produces mRNAs encoding the prototype PTB protein, a form of PTB protein containing 19 additional residues, and a truncated form of PTB protein with a novel carboxyl terminus. A murine homolog of pPTB has been characterized previously as a DNA-binding protein. Sequence comparisons indicate that pPTB is distantly related to the hnRNP L protein and that these two proteins should be considered as members of a novel family of RNA-binding proteins.

Bibliography

Gil, A., Sharp, P. A., Jamison, S. F., & Garcia-Blanco, M. A. (1991). Characterization of cDNAs encoding the polypyrimidine tract-binding protein. Genes & Development, 5(7), 1224â1236.

Authors 4

A Gil (first)
P A Sharp (additional)
S F Jamison (additional)
M A Garcia-Blanco (additional)

References 70 Referenced 180

{'key': '2021111320183743000_5.7.1224.1', 'first-page': '2932', 'article-title': 'mRNA polyadenylate-binding protein: Gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.', 'volume': '6', 'year': '1986', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / mRNA polyadenylate-binding protein: Gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence. (1986)
10.1073/pnas.84.20.6970
10.1006/jmbi.1990.9999
10.1038/342819a0
10.1101/gad.3.4.431
10.1016/0092-8674(88)90248-6
10.1016/0092-8674(85)90270-3
10.1016/0092-8674(87)90332-1
Bothwell, A.L.M., D.W. Ballard, and W.M. Philbrick. 1990. 25 kd Nuclear protein found in plasmacytoma. EMBL/Genbank Databases EMBL; X52101; MMP25K.
10.1146/annurev.bi.56.070187.002343
{'key': '2021111320183743000_5.7.1224.11', 'first-page': '1344', 'article-title': 'The “spliceosome”: Yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction.', 'volume': '228', 'year': '1985', 'journal-title': 'Science'} / Science / The “spliceosome”: Yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction. (1985)
10.1016/S0021-9258(18)60904-4 / J. Biol. Chem. / RNA binding fragments from nucleolin contain the ribonucleoprotein consensus sequence. (1987)
10.1073/pnas.86.24.9788
10.1126/science.3952495
10.1016/0968-0004(88)90046-1
10.1002/j.1460-2075.1988.tb03330.x / EMBO J. / Structure and expression of a Xenopus gene encoding an snRNP protein (U1 70K). (1988)
10.1016/S0092-8674(85)80131-8
10.1038/343437a0
10.1101/gad.3.12a.1874
10.1073/pnas.87.8.3082
10.1016/0092-8674(90)90236-8
10.1016/0092-8674(86)90812-3
10.1016/S0092-8674(85)80130-6
10.1146/annurev.ge.20.120186.003323
{'key': '2021111320183743000_5.7.1224.25', 'first-page': '787', 'article-title': 'Isolation and characterization of full-length cDNA clones for human α-, β-, and γ-actin mRNAs: Skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.', 'volume': '3', 'year': '1983', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Isolation and characterization of full-length cDNA clones for human α-, β-, and γ-actin mRNAs: Skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. (1983)
10.1093/nar/17.14.5633
10.1016/S0021-9258(17)33665-7 / J. Biol. Chem. / Purification and physical characterization of nucleic acid helix-unwinding proteins from calf thymus. (1976)
10.1038/344461a0
10.1016/0092-8674(86)90066-8
10.1016/0092-8674(84)90268-X
10.1093/nar/15.20.8125
10.1016/0092-8674(85)90269-7
10.1101/gad.4.7.1158
10.1016/0092-8674(90)90237-9
10.1101/gad.2.9.1155
10.1002/j.1460-2075.1985.tb04119.x / EMBO J. / Purification of a protein required for the splicing of premRNA and its separation from the lariat debranching enzyme. (1985)
10.1016/0092-8674(89)90882-9
10.1038/325673a0
Maniatis, T., E.F. Fritsch, and J. Sambrook. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
10.1016/0167-4781(90)90032-W / Biochim. Biophys Acta / Structure and developmental expression of chicken nucleolin and NO38: Coordinate expression of two abundant non-ribosomal nucleolar proteins. (1990)
10.1093/nar/14.7.3045
10.1038/348515a0
10.1146/annurev.bi.55.070186.005351
10.1016/0092-8674(91)90219-O
10.1083/jcb.109.6.2575
{'key': '2021111320183743000_5.7.1224.46', 'first-page': '8742', 'article-title': 'The mammalian analogue of the yeast prp8 splicing protein is present in the U4/5/6 small nuclear ribonucleoproteins.', 'volume': '80', 'year': '1989', 'journal-title': 'Proc. Natl. Acad. Sci.'} / Proc. Natl. Acad. Sci. / The mammalian analogue of the yeast prp8 splicing protein is present in the U4/5/6 small nuclear ribonucleoproteins. (1989)
10.1038/263211a0
10.1016/0092-8674(89)90175-X
10.1101/gad.3.12b.2113
10.1126/science.3144044
10.1126/science.2973660
{'key': '2021111320183743000_5.7.1224.52', 'first-page': '207', 'article-title': 'A factor U2AF is required for the U2 snRNP binding and splicing complex assembly.', 'volume': '52', 'year': '1989', 'journal-title': 'Cell'} / Cell / A factor U2AF is required for the U2 snRNP binding and splicing complex assembly. (1989)
10.1016/0092-8674(86)90557-X
10.1073/pnas.74.12.5463
10.1016/0092-8674(89)90296-1
10.1002/j.1460-2075.1988.tb03101.x / EMBO J. / A U1 snRNA: Pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site. (1988)
10.1126/science.2161558
10.1001/jama.1988.03410200091032
{'key': '2021111320183743000_5.7.1224.59', 'first-page': '1258', 'article-title': '5′-Splice site selection in yeast: Genetic alterations in base-pairing with U1 reveal additional requirements.', 'volume': '2', 'year': '1989', 'journal-title': 'Genes & Dev.'} / Genes & Dev. / 5′-Splice site selection in yeast: Genetic alterations in base-pairing with U1 reveal additional requirements. (1989)
10.1038/333568a0
10.1016/0092-8674(89)90678-8
10.1016/0092-8674(89)90426-1
10.1002/j.1460-2075.1988.tb03228.x / EMBO J. / RNA binding specificity of hnRNP proteins: A subset binds to the 3′ end of introns. (1988)
{'key': '2021111320183743000_5.7.1224.64', 'first-page': '1731', 'article-title': 'Primary structure of human nuclear ribonucleoprotein particle C proteins: Conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA and pre-rRNA-binding proteins.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Primary structure of human nuclear ribonucleoprotein particle C proteins: Conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA and pre-rRNA-binding proteins. (1987)
10.1016/0092-8674(86)90518-0
10.1002/j.1460-2075.1990.tb07634.x / EMBO J. / Splicing factor SF4 is dispensable for the assembly of a functional splicing complex and participates in the subsequent steps of the splicing reaction. (1990)
10.1016/0092-8674(84)90426-4
10.1073/pnas.86.23.9243
10.1002/j.1460-2075.1991.tb07937.x / EMBO J. / Biochemical characterization of U2 snRNP auxiliary factor: An essential pre-mRNA splicing factor with a novel intranuclear distribution. (1991)
10.1093/nar/17.7.2655

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:20 p.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:38 p.m.)
Issued	34 years, 1 month ago (July 1, 1991)
Published	34 years, 1 month ago (July 1, 1991)
Published Online	34 years, 1 month ago (July 1, 1991)
Published Print	34 years, 1 month ago (July 1, 1991)

Funders 0

None

BibTeX

@article{Gil_1991, title={Characterization of cDNAs encoding the polypyrimidine tract-binding protein.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.7.1224}, DOI={10.1101/gad.5.7.1224}, number={7}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Gil, A and Sharp, P A and Jamison, S F and Garcia-Blanco, M A}, year={1991}, month=jul, pages={1224–1236} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        15
      ]
    ],
    "date-time": "2025-07-15T03:38:46Z",
    "timestamp": 1752550726626
  },
  "reference-count": 70,
  "publisher": "Cold Spring Harbor Laboratory",
  "issue": "7",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1991,
        7
      ]
    ]
  },
  "abstract": "<jats:p>The polypyrimidine tract of mammalian introns is recognized by a 62-kD protein (pPTB). Mutations in the polypyrimidine tract that reduce the binding of pPTB also reduce the efficiency of formation of the pre-spliceosome complex containing U2 snRNP. The PTB protein was purified to homogeneity by affinity chromatography on a matrix containing poly(U), and peptide sequence was used to isolate several cDNAs. Because a variety of cell types express mRNA complementary to these cDNAs, PTB may be a ubiquitous splicing factor. Three classes of cDNAs were identified, on the basis of the presence of additional sequences at an internal position. This variation in sequence probably reflects alternative splicing of the PTB pre-mRNA and produces mRNAs encoding the prototype PTB protein, a form of PTB protein containing 19 additional residues, and a truncated form of PTB protein with a novel carboxyl terminus. A murine homolog of pPTB has been characterized previously as a DNA-binding protein. Sequence comparisons indicate that pPTB is distantly related to the hnRNP L protein and that these two proteins should be considered as members of a novel family of RNA-binding proteins.</jats:p>",
  "DOI": "10.1101/gad.5.7.1224",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        6,
        5
      ]
    ],
    "date-time": "2007-06-05T21:15:46Z",
    "timestamp": 1181078146000
  },
  "page": "1224-1236",
  "source": "Crossref",
  "is-referenced-by-count": 180,
  "title": "Characterization of cDNAs encoding the polypyrimidine tract-binding protein.",
  "prefix": "10.1101",
  "volume": "5",
  "author": [
    {
      "given": "A",
      "family": "Gil",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "P A",
      "family": "Sharp",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "S F",
      "family": "Jamison",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M A",
      "family": "Garcia-Blanco",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "246",
  "published-online": {
    "date-parts": [
      [
        1991,
        7,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2021111320183743000_5.7.1224.1",
      "first-page": "2932",
      "article-title": "mRNA polyadenylate-binding protein: Gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.",
      "volume": "6",
      "year": "1986",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111320183743000_5.7.1224.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.84.20.6970"
    },
    {
      "key": "2021111320183743000_5.7.1224.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1990.9999"
    },
    {
      "key": "2021111320183743000_5.7.1224.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/342819a0"
    },
    {
      "key": "2021111320183743000_5.7.1224.5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.3.4.431"
    },
    {
      "key": "2021111320183743000_5.7.1224.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(88)90248-6"
    },
    {
      "key": "2021111320183743000_5.7.1224.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(85)90270-3"
    },
    {
      "key": "2021111320183743000_5.7.1224.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(87)90332-1"
    },
    {
      "key": "2021111320183743000_5.7.1224.9",
      "unstructured": "Bothwell, A.L.M., D.W. Ballard, and W.M. Philbrick. 1990. 25 kd Nuclear protein found in plasmacytoma. EMBL/Genbank Databases EMBL; X52101; MMP25K."
    },
    {
      "key": "2021111320183743000_5.7.1224.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.56.070187.002343"
    },
    {
      "key": "2021111320183743000_5.7.1224.11",
      "first-page": "1344",
      "article-title": "The \u201cspliceosome\u201d: Yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction.",
      "volume": "228",
      "year": "1985",
      "journal-title": "Science"
    },
    {
      "key": "2021111320183743000_5.7.1224.12",
      "doi-asserted-by": "crossref",
      "first-page": "10922",
      "DOI": "10.1016/S0021-9258(18)60904-4",
      "article-title": "RNA binding fragments from nucleolin contain the ribonucleoprotein consensus sequence.",
      "volume": "262",
      "year": "1987",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111320183743000_5.7.1224.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.86.24.9788"
    },
    {
      "key": "2021111320183743000_5.7.1224.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3952495"
    },
    {
      "key": "2021111320183743000_5.7.1224.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0968-0004(88)90046-1"
    },
    {
      "key": "2021111320183743000_5.7.1224.16",
      "doi-asserted-by": "crossref",
      "first-page": "4311",
      "DOI": "10.1002/j.1460-2075.1988.tb03330.x",
      "article-title": "Structure and expression of a Xenopus gene encoding an snRNP protein (U1 70K).",
      "volume": "7",
      "year": "1988",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111320183743000_5.7.1224.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(85)80131-8"
    },
    {
      "key": "2021111320183743000_5.7.1224.18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/343437a0"
    },
    {
      "key": "2021111320183743000_5.7.1224.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.3.12a.1874"
    },
    {
      "key": "2021111320183743000_5.7.1224.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.8.3082"
    },
    {
      "key": "2021111320183743000_5.7.1224.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90236-8"
    },
    {
      "key": "2021111320183743000_5.7.1224.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90812-3"
    },
    {
      "key": "2021111320183743000_5.7.1224.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(85)80130-6"
    },
    {
      "key": "2021111320183743000_5.7.1224.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.ge.20.120186.003323"
    },
    {
      "key": "2021111320183743000_5.7.1224.25",
      "first-page": "787",
      "article-title": "Isolation and characterization of full-length cDNA clones for human \u03b1-, \u03b2-, and \u03b3-actin mRNAs: Skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.",
      "volume": "3",
      "year": "1983",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111320183743000_5.7.1224.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/17.14.5633"
    },
    {
      "key": "2021111320183743000_5.7.1224.27",
      "doi-asserted-by": "crossref",
      "first-page": "2124",
      "DOI": "10.1016/S0021-9258(17)33665-7",
      "article-title": "Purification and physical characterization of nucleic acid helix-unwinding proteins from calf thymus.",
      "volume": "251",
      "year": "1976",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2021111320183743000_5.7.1224.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/344461a0"
    },
    {
      "key": "2021111320183743000_5.7.1224.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90066-8"
    },
    {
      "key": "2021111320183743000_5.7.1224.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(84)90268-X"
    },
    {
      "key": "2021111320183743000_5.7.1224.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/15.20.8125"
    },
    {
      "key": "2021111320183743000_5.7.1224.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(85)90269-7"
    },
    {
      "key": "2021111320183743000_5.7.1224.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.4.7.1158"
    },
    {
      "key": "2021111320183743000_5.7.1224.34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90237-9"
    },
    {
      "key": "2021111320183743000_5.7.1224.35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.2.9.1155"
    },
    {
      "key": "2021111320183743000_5.7.1224.36",
      "doi-asserted-by": "crossref",
      "first-page": "3571",
      "DOI": "10.1002/j.1460-2075.1985.tb04119.x",
      "article-title": "Purification of a protein required for the splicing of premRNA and its separation from the lariat debranching enzyme.",
      "volume": "4",
      "year": "1985",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111320183743000_5.7.1224.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90882-9"
    },
    {
      "key": "2021111320183743000_5.7.1224.38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/325673a0"
    },
    {
      "key": "2021111320183743000_5.7.1224.39",
      "unstructured": "Maniatis, T., E.F. Fritsch, and J. Sambrook. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York."
    },
    {
      "key": "2021111320183743000_5.7.1224.40",
      "doi-asserted-by": "crossref",
      "first-page": "126",
      "DOI": "10.1016/0167-4781(90)90032-W",
      "article-title": "Structure and developmental expression of chicken nucleolin and NO38: Coordinate expression of two abundant non-ribosomal nucleolar proteins.",
      "volume": "1049",
      "year": "1990",
      "journal-title": "Biochim. Biophys Acta"
    },
    {
      "key": "2021111320183743000_5.7.1224.41",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/14.7.3045"
    },
    {
      "key": "2021111320183743000_5.7.1224.42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/348515a0"
    },
    {
      "key": "2021111320183743000_5.7.1224.43",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.55.070186.005351"
    },
    {
      "key": "2021111320183743000_5.7.1224.44",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90219-O"
    },
    {
      "key": "2021111320183743000_5.7.1224.45",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.109.6.2575"
    },
    {
      "key": "2021111320183743000_5.7.1224.46",
      "first-page": "8742",
      "article-title": "The mammalian analogue of the yeast prp8 splicing protein is present in the U4/5/6 small nuclear ribonucleoproteins.",
      "volume": "80",
      "year": "1989",
      "journal-title": "Proc. Natl. Acad. Sci."
    },
    {
      "key": "2021111320183743000_5.7.1224.47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/263211a0"
    },
    {
      "key": "2021111320183743000_5.7.1224.48",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90175-X"
    },
    {
      "key": "2021111320183743000_5.7.1224.49",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.3.12b.2113"
    },
    {
      "key": "2021111320183743000_5.7.1224.50",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3144044"
    },
    {
      "key": "2021111320183743000_5.7.1224.51",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.2973660"
    },
    {
      "key": "2021111320183743000_5.7.1224.52",
      "first-page": "207",
      "article-title": "A factor U2AF is required for the U2 snRNP binding and splicing complex assembly.",
      "volume": "52",
      "year": "1989",
      "journal-title": "Cell"
    },
    {
      "key": "2021111320183743000_5.7.1224.53",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90557-X"
    },
    {
      "key": "2021111320183743000_5.7.1224.54",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.74.12.5463"
    },
    {
      "key": "2021111320183743000_5.7.1224.55",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90296-1"
    },
    {
      "key": "2021111320183743000_5.7.1224.56",
      "doi-asserted-by": "crossref",
      "first-page": "2533",
      "DOI": "10.1002/j.1460-2075.1988.tb03101.x",
      "article-title": "A U1 snRNA: Pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5\u2032 cleavage site.",
      "volume": "7",
      "year": "1988",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111320183743000_5.7.1224.57",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.2161558"
    },
    {
      "key": "2021111320183743000_5.7.1224.58",
      "doi-asserted-by": "publisher",
      "DOI": "10.1001/jama.1988.03410200091032"
    },
    {
      "key": "2021111320183743000_5.7.1224.59",
      "first-page": "1258",
      "article-title": "5\u2032-Splice site selection in yeast: Genetic alterations in base-pairing with U1 reveal additional requirements.",
      "volume": "2",
      "year": "1989",
      "journal-title": "Genes & Dev."
    },
    {
      "key": "2021111320183743000_5.7.1224.60",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/333568a0"
    },
    {
      "key": "2021111320183743000_5.7.1224.61",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90678-8"
    },
    {
      "key": "2021111320183743000_5.7.1224.62",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90426-1"
    },
    {
      "key": "2021111320183743000_5.7.1224.63",
      "doi-asserted-by": "crossref",
      "first-page": "3519",
      "DOI": "10.1002/j.1460-2075.1988.tb03228.x",
      "article-title": "RNA binding specificity of hnRNP proteins: A subset binds to the 3\u2032 end of introns.",
      "volume": "7",
      "year": "1988",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111320183743000_5.7.1224.64",
      "first-page": "1731",
      "article-title": "Primary structure of human nuclear ribonucleoprotein particle C proteins: Conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA and pre-rRNA-binding proteins.",
      "volume": "7",
      "year": "1987",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2021111320183743000_5.7.1224.65",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90518-0"
    },
    {
      "key": "2021111320183743000_5.7.1224.66",
      "doi-asserted-by": "crossref",
      "first-page": "4119",
      "DOI": "10.1002/j.1460-2075.1990.tb07634.x",
      "article-title": "Splicing factor SF4 is dispensable for the assembly of a functional splicing complex and participates in the subsequent steps of the splicing reaction.",
      "volume": "9",
      "year": "1990",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111320183743000_5.7.1224.67",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(84)90426-4"
    },
    {
      "key": "2021111320183743000_5.7.1224.68",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.86.23.9243"
    },
    {
      "key": "2021111320183743000_5.7.1224.69",
      "doi-asserted-by": "crossref",
      "first-page": "207",
      "DOI": "10.1002/j.1460-2075.1991.tb07937.x",
      "article-title": "Biochemical characterization of U2 snRNP auxiliary factor: An essential pre-mRNA splicing factor with a novel intranuclear distribution.",
      "volume": "10",
      "year": "1991",
      "journal-title": "EMBO J."
    },
    {
      "key": "2021111320183743000_5.7.1224.70",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/17.7.2655"
    }
  ],
  "container-title": "Genes &amp; Development",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1101/gad.5.7.1224",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        11,
        14
      ]
    ],
    "date-time": "2021-11-14T04:20:51Z",
    "timestamp": 1636863651000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "http://genesdev.cshlp.org/lookup/doi/10.1101/gad.5.7.1224"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1991,
        7
      ]
    ]
  },
  "references-count": 70,
  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          1991,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1101/gad.5.7.1224"
  ],
  "URL": "http://dx.doi.org/10.1101/gad.5.7.1224",
  "relation": {},
  "ISSN": [
    "0890-9369",
    "1549-5477"
  ],
  "subject": [],
  "container-title-short": "Genes Dev.",
  "published": {
    "date-parts": [
      [
        1991,
        7
      ]
    ]
  }
}