Alternative splicing of fosB transcripts results in differentially expressed mRNAs encoding functionally antagonistic proteins.
10.1101/gad.5.7.1212
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

We show that serum-stimulated fibroblasts transiently express two different forms of fosB mRNA, which are generated by alternative splicing of the transcript from a single gene. In addition to the known long form (fosB-L), encoding a protein of 338 amino acids (FosB-L), a second shorter form (fosB-S) with a deletion of 140 bp was detected. This deletion creates a stop codon 3' to the leucine repeat, giving rise to a protein of 237 amino acids (FosB-S) lacking the carboxyl terminus of FosB-L. Only the long FosB form efficiently induces transformation in mouse and rat fibroblast cell lines and trans-represses the c-fos promoter. Both of these functions are suppressed by coexpressed FosB-S. Upon serum stimulation, maximum expression of the oncogenic fosB-L form precedes the expression of the antagonistic fosB-S form, indicating a new mechanisms regulating the action of members of the Fos family. However, FosB-L and FosB-S do not differ in all trans-regulatory properties: Trans-activation of a 5x TRE-CAT reporter construct in HeLa and NIH-3T3 cells was found with both FosB forms. These observations suggest a correlation between fosB-induced transformation and trans-repression, thus pointing to different mechanisms involved in transformation by fosB and c-fos/v-fos.

Bibliography

Mumberg, D., Lucibello, F. C., Schuermann, M., & Müller, R. (1991). Alternative splicing of fosB transcripts results in differentially expressed mRNAs encoding functionally antagonistic proteins. Genes & Development, 5(7), 1212–1223.

Authors 4
  1. D Mumberg (first)
  2. F C Lucibello (additional)
  3. M Schuermann (additional)
  4. R Müller (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:20 p.m.)
Indexed 2 months, 1 week ago (June 21, 2025, 12:48 a.m.)
Issued 34 years, 2 months ago (July 1, 1991)
Published 34 years, 2 months ago (July 1, 1991)
Published Online 34 years, 2 months ago (July 1, 1991)
Published Print 34 years, 2 months ago (July 1, 1991)
Funders 0

None

@article{Mumberg_1991, title={Alternative splicing of fosB transcripts results in differentially expressed mRNAs encoding functionally antagonistic proteins.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.7.1212}, DOI={10.1101/gad.5.7.1212}, number={7}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Mumberg, D and Lucibello, F C and Schuermann, M and Müller, R}, year={1991}, month=jul, pages={1212–1223} }