DOI: 10.1101/gad.5.5.855. Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression.

Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression.

10.1101/gad.5.5.855

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

A complex array of activator and repressor elements located within 669 bp proximal to the fushi tarazu (ftz) transcriptional start site is sufficient to generate the "zebra-stripe" expression pattern characteristic of the ftz gene. P-element-mediated transformation and ftz promoter/lacZ fusion genes were used to characterize, in detail, several of these transcriptional control elements. By reconstructing promoters with synthetic oligonucleotides containing cis-regulators of stripe expression, we show that these regulatory sites can function as independent units to direct position-specific transcription in the Drosophila embryo. In particular, we demonstrate that multiple copies of a positive regulatory site can mediate expression in both the odd- and even-numbered parasegments throughout most of the germ band and that negative regulatory sites can transform a continuous pattern of gene expression into discrete stripes. The reconstructed promoter system presented provides an effective means of studying molecular mechanisms governing spatially restricted transcription in the early embryo.

Bibliography

Topol, J., Dearolf, C. R., Prakash, K., & Parker, C. S. (1991). Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression. Genes & Development, 5(5), 855â867.

Authors 4

J Topol (first)
C R Dearolf (additional)
K Prakash (additional)
C S Parker (additional)

References 44 Referenced 33

10.1038/msb.2012.72
10.1016/0092-8674(88)90088-8
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10.1016/0092-8674(86)90543-X
10.1242/dev.107.3.673 / Development / The zygotic control of Drosophila pair-rule gene expression II. Spatial repression by gap and pair-rule gene products. (1989)
10.1101/gad.2.7.883
10.1038/341340a0
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10.1016/0092-8674(87)90523-X
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10.1016/0092-8674(86)90018-8
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Prakash, K., J. Topol, C.D. Dearolf, and C.S. Parker. 1991. Transcriptional regulation during early Drosophila development. In Advances in developmental biochemistry (ed. P. Wassarman), vol. I. JAI Press, Greenwich. (In press.)
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Topol, J. 1990. Transcriptional control of the Drosophila segmentation gene fushi tarazu. Ph.D. thesis. California Institute of Technology, Pasadena.
Topol, J., G. Weiderrecht, and C.S. Parker. 1987. Biochemical analysis of the fushi tarazu and Ultrabithorax promoter regions. In Genetic regulation of development (ed. W. F. Loomis), pp 3–12. Alan R. Liss Inc., New York.
10.1101/gad.4.4.624
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10.1016/0092-8674(87)90201-7
10.1007/BF00848158

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:19 p.m.)
Indexed	1 year, 8 months ago (Dec. 2, 2023, 7:38 p.m.)
Issued	34 years, 4 months ago (May 1, 1991)
Published	34 years, 4 months ago (May 1, 1991)
Published Online	34 years, 4 months ago (May 1, 1991)
Published Print	34 years, 4 months ago (May 1, 1991)

Funders 0

None

BibTeX

@article{Topol_1991, title={Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.5.855}, DOI={10.1101/gad.5.5.855}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Topol, J and Dearolf, C R and Prakash, K and Parker, C S}, year={1991}, month=may, pages={855–867} }

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