DOI: 10.1101/gad.5.5.773. Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.

Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.

10.1101/gad.5.5.773

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

U4 and U6 small nuclear RNAs are associated by an extensive base-pairing interaction that must be disrupted and reformed with each round of splicing. U4 mutations within the U4/U6 interaction domain destabilize the complex in vitro and cause a cold-sensitive phenotype in vivo. Restabilization of the U4/U6 helix by dominant (gain-of-function), compensatory mutations in U6 results in wild-type growth. Cold-insensitive growth can also be restored by two classes of recessive (loss-of-function) suppressors: (1) mutations in PRP24, which we show to be a U6-specific binding protein of the RNP-consensus family; and (2) mutations in U6, which lie outside the interaction domain and identify putative PRP24-binding sites. Destabilization of the U4/U6 helix causes the accumulation of a PRP24/U4/U6 complex, which is undetectable in wild-type cells. The loss-of-function suppressor mutations inhibit the binding of PRP24 to U6, and thus presumably promote the release of PRP24 from the PRP24/U4/U6 complex and the reformation of the base-paired U4/U6 snRNP. We propose that the PRP24/U4/U6 complex is normally a highly transient intermediate in the spliceosome cycle and that PRP24 promotes the reannealing of U6 with U4.

Bibliography

Shannon, K. W., & Guthrie, C. (1991). Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs. Genes & Development, 5(5), 773â785.

Authors 2

K W Shannon (first)
C Guthrie (additional)

References 58 Referenced 103

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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:18 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 6, 2025, 8:54 a.m.)
Issued	34 years, 3 months ago (May 1, 1991)
Published	34 years, 3 months ago (May 1, 1991)
Published Online	34 years, 3 months ago (May 1, 1991)
Published Print	34 years, 3 months ago (May 1, 1991)

Funders 0

None

BibTeX

@article{Shannon_1991, title={Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.5.773}, DOI={10.1101/gad.5.5.773}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Shannon, K W and Guthrie, C}, year={1991}, month=may, pages={773–785} }

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