A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.
10.1101/gad.5.4.629
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which the first step of mRNA splicing is inhibited. The growth and splicing defects are recessive and cosegregate, thus defining a single essential gene (PRP28). The wild-type PRP28 gene was cloned, and sequence analysis reveals extensive homology to a family of proteins that are thought to function as ATP-dependent RNA helicases. The cold sensitivity is caused by a glycine-to-glutamic acid change in a conserved sequence motif. Interestingly, double mutants containing conditional alleles of PRP28 and PRP24, which encodes a U6 snRNA-binding protein, are inviable. In addition, a suppressor of prp28-1 is a mutant allele of PRP8, which encodes a U5 protein, thus linking PRP28 with U5. These data are consistent with a scenario in which PRP28 acts to unwind the U4/U6 base-pairing interaction in the U4/U6/U5 snRNP, facilitating the first covalent step of splicing.

Bibliography

Strauss, E. J., & Guthrie, C. (1991). A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Genes & Development, 5(4), 629–641.

Authors 2
  1. E J Strauss (first)
  2. C Guthrie (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:19 p.m.)
Indexed 1 year ago (Aug. 8, 2024, 3:55 a.m.)
Issued 34 years, 5 months ago (April 1, 1991)
Published 34 years, 5 months ago (April 1, 1991)
Published Online 34 years, 5 months ago (April 1, 1991)
Published Print 34 years, 5 months ago (April 1, 1991)
Funders 0

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@article{Strauss_1991, title={A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.4.629}, DOI={10.1101/gad.5.4.629}, number={4}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Strauss, E J and Guthrie, C}, year={1991}, month=apr, pages={629–641} }