RAP1 protein activates and silences transcription of mating-type genes in yeast.
10.1101/gad.5.4.616
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

RAP1 is a sequence-specific DNA-binding protein essential for cell growth. The occurrence of RAP1-binding sites in many promoter regions, the mating-type gene silencer elements, and telomeres suggests that RAP1 has multiple functions in the cell. To assess its role in transcription, temperature-sensitive mutations in RAP1 were generated. Analysis of rap1ts strains provides evidence that RAP1 functions in both transcriptional activation and silencing of mating-type genes. Several observations indicate that rap1ts strains are defective in the expression of MAT alpha, whose upstream activation sequence (UAS) contains a RAP1-binding site. At nonpermissive temperatures, decreases in MAT alpha steady-state transcript levels can be detected in MAT alpha rap1ts strains. Furthermore, these strains are deficient in alpha-pheromone production and simultaneously express at least two alpha-specific genes. These phenotypes can be reversed by replacing the RAP1-binding site at MAT alpha with a binding site for the GAL4 transcriptional activator. Certain rap1ts alleles have an opposite effect on the silent mating-type locus HMR, which becomes partially derepressed at nonpermissive temperatures.

Bibliography

Kurtz, S., & Shore, D. (1991). RAP1 protein activates and silences transcription of mating-type genes in yeast. Genes & Development, 5(4), 616–628.

Authors 2
  1. S Kurtz (first)
  2. D Shore (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:18 p.m.)
Indexed 1 month, 2 weeks ago (July 14, 2025, 11:47 p.m.)
Issued 34 years, 4 months ago (April 1, 1991)
Published 34 years, 4 months ago (April 1, 1991)
Published Online 34 years, 4 months ago (April 1, 1991)
Published Print 34 years, 4 months ago (April 1, 1991)
Funders 0

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@article{Kurtz_1991, title={RAP1 protein activates and silences transcription of mating-type genes in yeast.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.4.616}, DOI={10.1101/gad.5.4.616}, number={4}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Kurtz, S and Shore, D}, year={1991}, month=apr, pages={616–628} }