DOI: 10.1101/gad.5.2.244. Occlusion of the HIV poly(A) site.

10.1101/gad.5.2.244

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

To investigate the selective use of poly(A) sites in the 3' long terminal repeat (LTR) but not the 5' LTR of retroviruses, we have studied the poly(A) site of the human immunodeficiency virus (HIV-1). Using hybrid HIV/alpha-globin gene constructs, we demonstrate that the HIV poly(A) site is inactive or occluded when adjacent to an active promoter, either the homologous HIV promoter or the alpha-globin gene promoter. Furthermore, this occlusion of the HIV poly(A) site occurs over a considerable distance of up to at least 500 bp. In contrast, two nonretroviral poly(A) sites [alpha-globin and a synthetic poly(A) site] are active when close to a promoter. We also show that a short fragment of approximately 60 nucleotides containing the HIV poly(A) site is fully active when placed at the 3' end of the human alpha-globin gene or within the rabbit beta-globin gene. This result rules out the requirement of more distant upstream elements for the activity of the HIV poly(A) site, as has been suggested for other viral poly(A) sites. Finally, we show that the GT-rich downstream region of the HIV poly(A) site confers poly(A) site occlusion properties on a synthetic poly(A) site. This result focuses attention on this more variable part of a poly(A) site in retroviruses as a possible general signal for poly(A) site occlusion.

Bibliography

Weichs an der Glon, C., Monks, J., & Proudfoot, N. J. (1991). Occlusion of the HIV poly(A) site. Genes & Development, 5(2), 244â253.

Authors 3

C Weichs an der Glon (first)
J Monks (additional)
N J Proudfoot (additional)

References 23 Referenced 52

10.1016/0092-8674(88)90113-4
10.1128/jvi.63.1.421-424.1989 / J. Virol. / Identification of a U5-specific sequence required for efficient polyadenylation within the human immunodeficiency virus long terminal repeat. (1989)
10.1016/0092-8674(89)90420-0
10.1038/307241a0
10.1016/0092-8674(87)90292-3
10.1038/295120a0
10.1101/gad.3.2.212
10.1038/306398a0
10.1016/0168-9525(88)90028-5
{'key': '2021111320162623000_5.2.244.10', 'first-page': '4008', 'article-title': 'Tripartite sequences within and 3â\x80² to the sea urchin H2A histone gene display properties associated with a transcriptional termination process.', 'volume': '6', 'year': '1986', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Tripartite sequences within and 3â² to the sea urchin H2A histone gene display properties associated with a transcriptional termination process. (1986)
{'key': '2021111320162623000_5.2.244.11', 'first-page': '127', 'article-title': 'HIV trans-activation and transcriptional control mechanisms.', 'volume': '1', 'year': '1989', 'journal-title': 'The New Biologist'} / The New Biologist / HIV trans-activation and transcriptional control mechanisms. (1989)
10.1016/S0065-3527(08)60451-0 / Adv. Virus Res. / The role of avian retroviral LTRs in the regulation of gene expression and viral replication. (1985)
10.1101/gad.3.7.1019
10.1093/nar/12.18.7035
10.1016/0092-8674(80)90347-5
10.1002/j.1460-2075.1984.tb02007.x / EMBO J. / Transcriptional analysis of human zeta globin genes. (1984)
{'key': '2021111320162623000_5.2.244.17', 'first-page': '57', 'article-title': 'Complete nucleotide sequences of functional clones of the AIDS virus.', 'volume': '3', 'year': '1987', 'journal-title': 'AIDS Res.'} / AIDS Res. / Complete nucleotide sequences of functional clones of the AIDS virus. (1987)
10.1101/gad.4.5.764
10.1038/346081a0
10.1126/science.3287617
10.1002/j.1460-2075.1986.tb04587.x / EMBO J. / Î±-Thalassaemia caused by a poly(A) site mutation reveals that transcriptional termination is linked to 3â² end processing in the human Î¶ globin gene. (1986)
{'key': '2021111320162623000_5.2.244.22', 'first-page': '241', 'article-title': 'Transcriptional promiscuity of the human Î±-globin gene.', 'volume': '9', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Transcriptional promiscuity of the human Î±-globin gene. (1989)
10.1126/science.6208611

Dates

Type	When
Created	18 years, 3 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:20 p.m.)
Indexed	1 year, 5 months ago (March 9, 2024, 5:49 a.m.)
Issued	34 years, 7 months ago (Feb. 1, 1991)
Published	34 years, 7 months ago (Feb. 1, 1991)
Published Online	34 years, 7 months ago (Feb. 1, 1991)
Published Print	34 years, 7 months ago (Feb. 1, 1991)

Funders 0

None

BibTeX

@article{Weichs_an_der_Glon_1991, title={Occlusion of the HIV poly(A) site.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.2.244}, DOI={10.1101/gad.5.2.244}, number={2}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Weichs an der Glon, C and Monks, J and Proudfoot, N J}, year={1991}, month=feb, pages={244–253} }

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