Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein.
10.1101/gad.5.2.151
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

Primary rat embryo fibroblasts were transformed by a p53 mutant (alanine to valine change at amino acid 135) plus ras. This p53val135 mutant is temperature sensitive for a conformational change detected by the binding of a monoclonal antibody, PAb246, which recognizes the wild-type protein or the great majority of p53val135 at 32.5 degrees C. At 37 degrees C, both mutant and wild-type p53 conformational forms co-exist in the cells, while at 39.5 degrees C, the majority of the p53val135 in the cell is in a mutant conformation not recognized by PAb246 antibody. At 39.5 degrees C, the mutant p53 is localized in the cytoplasm of the cell. At 32.5 degrees C, the p53 protein enters the nucleus and stops the growth of these cells. At 37 degrees C where there is a mixture of mutant and wild-type p53, the wild-type p53 protein is in a complex with hsc70 and mutant p53 protein in the cytoplasm of the cell during G1. This wild-type protein enters the nucleus as the cells enter the S-phase of the cell cycle. At 32.5 degrees C, the cells stop replication and arrest at the G1/S border. After 48 hr at 32.5 degrees C, 91% of the cells are in the G1 fraction of the cell cycle. The S-phase cells appear to be immune to the p53 negative regulation of growth until they enter the next G1 period.(ABSTRACT TRUNCATED AT 250 WORDS)

Bibliography

Martinez, J., Georgoff, I., Martinez, J., & Levine, A. J. (1991). Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein. Genes & Development, 5(2), 151–159.

Authors 4
  1. J Martinez (first)
  2. I Georgoff (additional)
  3. J Martinez (additional)
  4. A J Levine (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:16 p.m.)
Indexed 5 days, 22 hours ago (Aug. 20, 2025, 9:02 a.m.)
Issued 34 years, 6 months ago (Feb. 1, 1991)
Published 34 years, 6 months ago (Feb. 1, 1991)
Published Online 34 years, 6 months ago (Feb. 1, 1991)
Published Print 34 years, 6 months ago (Feb. 1, 1991)
Funders 0

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@article{Martinez_1991, title={Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.2.151}, DOI={10.1101/gad.5.2.151}, number={2}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Martinez, J and Georgoff, I and Martinez, J and Levine, A J}, year={1991}, month=feb, pages={151–159} }