Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast.
10.1101/gad.5.11.2000
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The yeast HO gene is transcribed transiently during G1 as cells undergo START. START-specific HO activation requires two proteins, SWI4 and SWI6, which act via a motif (CACGA4) repeated up to 10 times within the URS2 region of the HO promoter. We identified a DNA-binding activity containing SWI4 and SWI6 that recognizes the CACGA4 sequences within URS2. Two forms of SWI4,6-DNA complexes called L and U can be distinguished by their electrophoretic mobility. L complexes can be detected at all stages of the cell cycle, but U complexes are only detected in cells that have undergone START. The formation of U complexes may be the trigger of HO activation. The SWI6 protein is concentrated in the nucleus throughout G1, but at some point in S or G2 significant amounts accumulate in the cytoplasm. This change in cellular location of the SWI6 protein might contribute to the turnoff of HO transcription after cells have undergone START.

Bibliography

Taba, M. R., Muroff, I., Lydall, D., Tebb, G., & Nasmyth, K. (1991). Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast. Genes & Development, 5(11), 2000–2013.

Authors 5
  1. M R Taba (first)
  2. I Muroff (additional)
  3. D Lydall (additional)
  4. G Tebb (additional)
  5. K Nasmyth (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:23 p.m.)
Indexed 4 months, 2 weeks ago (April 11, 2025, 3:49 a.m.)
Issued 33 years, 9 months ago (Nov. 1, 1991)
Published 33 years, 9 months ago (Nov. 1, 1991)
Published Online 33 years, 9 months ago (Nov. 1, 1991)
Published Print 33 years, 9 months ago (Nov. 1, 1991)
Funders 0

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@article{Taba_1991, title={Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.11.2000}, DOI={10.1101/gad.5.11.2000}, number={11}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Taba, M R and Muroff, I and Lydall, D and Tebb, G and Nasmyth, K}, year={1991}, month=nov, pages={2000–2013} }