DOI: 10.1101/gad.5.10.1881. The dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos.

The dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos.

10.1101/gad.5.10.1881

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

A gradient of the maternal morphogen dorsal (dl) initiates the differentiation of various tissues along the dorsal-ventral axis of early Drosophila embryos. dl is a sequence-specific DNA-binding protein that is related to the mammalian regulatory factor NF-kappa B. Previous studies suggest that dl can function as a transcriptional repressor. To determine how dl functions as an activator we have examined the promoter of the mesoderm determinant gene twist (twi). Genetic studies suggest that peak levels of dl protein in ventral regions of early embryos initiate twi expression. Using a combination of promoter fusion-P-transformation assays, and in vitro DNA-binding assays coupled with site-directed mutagenesis, we establish a direct link between dl-binding sites and twi expression in the early embryo. We also present evidence that the dorsal-ventral limits of twi expression depend on the number and affinity of dl-binding sites present in its promoter. A comparison of twi with a second dl target gene, zen, suggests a correlation between the affinities of dl-binding sites and response to different thresholds of dl morphogen.

Bibliography

Jiang, J., Kosman, D., Ip, Y. T., & Levine, M. (1991). The dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos. Genes & Development, 5(10), 1881â1891.

Authors 4

J Jiang (first)
D Kosman (additional)
Y T Ip (additional)
M Levine (additional)

References 35 Referenced 306

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{'key': '2021111320194746000_5.10.1881.9', 'first-page': '956', 'article-title': 'Characterization of Drosophila transcription factors that activate the tandem promoters of the alcohol dehydrogenase gene.', 'volume': '41', 'year': '1985', 'journal-title': 'Cell'} / Cell / Characterization of Drosophila transcription factors that activate the tandem promoters of the alcohol dehydrogenase gene. (1985)
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Kosman, D., Ip, Y.T. Levine, M. and Arora. K. 1991. The establishment of the mesoderm-neuroectoderm boundary in the Drosophila embryo. Science (in press). (10.1126/science.1925551)
10.1016/0092-8674(89)90935-5
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10.1002/j.1460-2075.1988.tb03056.x / EMBO J. / Sequence of the twist gene and nuclear localization of its protein in endomesodermal cells of early Drosophila embryos. (1988)
10.1016/0092-8674(91)90014-P
10.1016/0378-1119(88)90177-1
10.1016/S0022-5193(69)80016-0

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:20 p.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:36 p.m.)
Issued	33 years, 10 months ago (Oct. 1, 1991)
Published	33 years, 10 months ago (Oct. 1, 1991)
Published Online	33 years, 10 months ago (Oct. 1, 1991)
Published Print	33 years, 10 months ago (Oct. 1, 1991)

Funders 0

None

BibTeX

@article{Jiang_1991, title={The dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos.}, volume={5}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.5.10.1881}, DOI={10.1101/gad.5.10.1881}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Jiang, J and Kosman, D and Ip, Y T and Levine, M}, year={1991}, month=oct, pages={1881–1891} }

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