DOI: 10.1101/gad.4.4.636. A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation.

A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation.

10.1101/gad.4.4.636

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

We created a library of DNA molecules in which the required TATA element of a yeast gal-his3 promoter is replaced by random-sequence oligomers averaging 16 bp in length. Surprisingly, 1% of such random sequences functionally replace the native yeast TATA element. In many cases, sequences completely unrelated to the consensus TATA element (TATAAA) stimulate transcription with equal or increased efficiency. Transcription mediated by these synthetic elements requires GAL4 and is initiated from normal his3 initiation sites, suggesting that it occurs by a mechanism indistinguishable from that involving wild-type TATA elements. Many, but not all, of these elements act as substrates for yeast TFIID in reconstituted transcription reactions in vitro. These observations indicate that yeast TFIID can stimulate transcription from TATA elements whose sequences differ from the consensus, and they suggest the possibility of alternative factors that may provide a related function for transcriptional activation.

Bibliography

Singer, V. L., Wobbe, C. R., & Struhl, K. (1990). A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation. Genes & Development, 4(4), 636â645.

Authors 3

V L Singer (first)
C R Wobbe (additional)
K Struhl (additional)

References 26 Referenced 145

10.1038/334037a0
10.1016/0092-8674(89)90578-3
10.1038/334077a0
10.1073/pnas.85.8.2691
10.1002/j.1460-2075.1989.tb03372.x / EMBO J. / Yeast upstream activator protein GCN4 can stimulate transcription when its binding site replaces the TATA element. (1989)
10.1016/0092-8674(87)90171-1
10.1016/0092-8674(88)90108-0
10.1016/0076-6879(83)01013-7
10.1016/S0092-8674(88)80020-5
10.1073/pnas.86.15.5718
{'key': '2021111320044362000_4.4.636.11', 'first-page': '5298', 'article-title': 'Functional distinctions between yeast TATA elements.', 'volume': '9', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Functional distinctions between yeast TATA elements. (1989)
10.1146/annurev.bi.57.070188.004203
10.1016/0378-1119(87)90131-4
10.1126/science.3289117
10.1146/annurev.ge.20.120186.003205
Miller, J.H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Oliphant, A. 1989. Defining genetic elements and protein structure by selecting functional sequences from random DNA. Ph.D. thesis, Harvard University, Cambridge.
10.1016/0076-6879(87)55037-6 / Methods Enzymol. / The use of random-sequence oligonucleotides for determining consensus sequences. (1987)
10.1016/0378-1119(86)90180-0 / Gene / Cloning of random-sequence oligodeoxynucleotides. (1986)
10.1016/0092-8674(87)90751-3
10.1038/337573a0
10.1016/S0021-9258(18)61505-4 / J. Biol. Chem. / Factors involved in specific transcription by mammalian RNA polymerase II: Purification and functional analysis of initiation factors IIB and IIE. (1987)
10.1101/gad.2.9.1144
10.1073/pnas.82.13.4394
{'key': '2021111320044362000_4.4.636.25', 'first-page': '3847', 'article-title': 'Constitutive and inducible Saccharomyces cerevisiae promoters: Evidence for two distinct molecular mechanisms.', 'volume': '6', 'year': '1986', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Constitutive and inducible Saccharomyces cerevisiae promoters: Evidence for two distinct molecular mechanisms. (1986)
10.1146/annurev.bi.58.070189.005155

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:05 p.m.)
Indexed	3 months, 3 weeks ago (May 8, 2025, 6:02 a.m.)
Issued	35 years, 5 months ago (April 1, 1990)
Published	35 years, 5 months ago (April 1, 1990)
Published Online	35 years, 5 months ago (April 1, 1990)
Published Print	35 years, 5 months ago (April 1, 1990)

Funders 0

None

BibTeX

@article{Singer_1990, title={A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation.}, volume={4}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.4.4.636}, DOI={10.1101/gad.4.4.636}, number={4}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Singer, V L and Wobbe, C R and Struhl, K}, year={1990}, month=apr, pages={636–645} }

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