DOI: 10.1101/gad.4.3.357. The Caenorhabditis elegans gene lin-10 is broadly expressed while required specifically for the determination of vulval cell fates.

The Caenorhabditis elegans gene lin-10 is broadly expressed while required specifically for the determination of vulval cell fates.

10.1101/gad.4.3.357

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

As a first step in a molecular dissection of the pathway controlling the determination of vulval cell fates in Caenorhabditis elegans, we have analyzed the gene lin-10. We show that loss-of-function mutations in this gene specifically prevent the induction of vulval cell lineages and result instead in the expression of hypodermal cell lineages. We isolated a transposon-insertion allele of lin-10 and used it to clone a genomic region that contains the lin-10 locus. The location of lin-10 within this region was determined by identifying a transcript affected by three independent lin-10 mutations and by delimiting the minimal segment of DNA sufficient to rescue the lin-10 mutant phenotype in germ line transformation experiments. The predicted lin-10 protein sequence is not similar to sequences in current data bases, suggesting that lin-10 defines a novel class of gene involved in the specification of cell fates. Although our genetic studies indicate that lin-10 is required specifically for the determination of vulval cell fates, lin-10 transcripts are present in cells other than vulval precursor cells. This result suggests that lin-10 may have a general but redundant role in development, functioning in diverse cell lineages to control cell fates. Alternatively, lin-10 may function specifically in vulval development, in which case lin-10 activity could be regulated at a post-transcriptional level or could have biological consequences only in conjunction with the products of other genes.

Bibliography

Kim, S. K., & Horvitz, H. R. (1990). The Caenorhabditis elegans gene lin-10 is broadly expressed while required specifically for the determination of vulval cell fates. Genes & Development, 4(3), 357â371.

Authors 2

S K Kim (first)
H R Horvitz (additional)

References 60 Referenced 53

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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:06 p.m.)
Indexed	2 months, 1 week ago (June 26, 2025, 2:04 p.m.)
Issued	35 years, 6 months ago (March 1, 1990)
Published	35 years, 6 months ago (March 1, 1990)
Published Online	35 years, 6 months ago (March 1, 1990)
Published Print	35 years, 6 months ago (March 1, 1990)

Funders 0

None

BibTeX

@article{Kim_1990, title={The Caenorhabditis elegans gene lin-10 is broadly expressed while required specifically for the determination of vulval cell fates.}, volume={4}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.4.3.357}, DOI={10.1101/gad.4.3.357}, number={3}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Kim, S K and Horvitz, H R}, year={1990}, month=mar, pages={357–371} }

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