DOI: 10.1101/gad.4.10.1790. Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3.

Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3.

10.1101/gad.4.10.1790

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Initiation factors are used by Escherichia coli to select the initiator tRNA over elongator tRNAs during translation initiation. IF3 appears to "inspect" the anticodon end of the tRNA, probably along with the initiation codon. The anticodon stem and loop of the initiator tRNA, together with part of the initiation codon of the mRNA, can be thought of as a unit. Changes made in the anticodon stem, the anticodon loop, or the anticodon of an initiator tRNA fragment result in a loss of selection by IF3 in an in vitro assay for translation initiation. IF3 allows the selection of an initiator tRNA anticodon stem and loop fragment on GUG and UUG codons but does not select that tRNA fragment in response to AUU.

Bibliography

Hartz, D., Binkley, J., Hollingsworth, T., & Gold, L. (1990). Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3. Genes & Development, 4(10), 1790â1800.

Authors 4

D Hartz (first)
J Binkley (additional)
T Hollingsworth (additional)
L Gold (additional)

References 43 Referenced 99

Beckett, D. and O.C. Uhlenbeck. 1984. Enzymatic synthesis of oligoribonucleotides. In Oligonucleotide synthesis a practical approach (ed. M.J. Gait), pp. 185–197. (IRL Press, Oxford.
10.1016/0167-4781(86)90111-9 / Biochim. Biophys. Acta / Formylmethionyl-tRNA binding to 30S ribosomes programmed with homopolynucleotides and the effect of translational initiation factor 3. (1986)
10.1002/j.1460-2075.1989.tb08515.x / EMBO J. / Dual translational initiation sites control function of lambda S gene. (1989)
Childs, J., K. Villanueba, D. Barrick, T.D. Schneider, G. Stormo, L. Gold, M. Leitner, and M. Caruthers. 1985. Ribosome binding site sequences and function. In Sequence Specificity in Transcription and Translation (ed. R. Calendar and L. Gold), pp. 341–350. Alan R. Liss, New York.
10.1126/science.3051379
10.1016/S0092-8674(88)80006-0
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10.1101/SQB.1969.034.01.023
10.1016/S0021-9258(17)42834-1 / J. Biol. Chem. / Initial velocity kinetic analysis of 30S initiation complex formation in an in vitro system derived from Escherichia coli. (1984)
10.1016/S0021-9258(19)42379-X / J. Biol. Chem. / Initiator methionine transfer ribonucleic acid from wheat embryo. Purification, properties, and partial nucleotide sequences. (1974)
10.1016/0022-2836(75)90215-6
10.1146/annurev.bi.57.070188.001215
10.1073/pnas.81.22.7061
Gualerzi, C. and C.L. Pon. 1981. Protein biosynthesis in procaryotic cells: Mechanism of 30S initiation complex formation in Escherichia coli. In Structural aspects of recognition and assembly in biological macromolecules (ed. L. Balaban, J.L. Sussman, W. Traub, and A. Yonath), pp. 805–826. Balaban, M. ISS, Rehovot.
10.1021/bi00627a025
10.1016/S0021-9258(17)30269-7 / J. Biol. Chem. / Mechanism of the spontaneous and initiation factor 3-induced dissociation of 30S aminoacyl-tRNA polynucleotide ternary complexes. (1979)
10.1016/S0076-6879(88)64058-4
10.1101/gad.3.12a.1899
10.1016/S0021-9258(18)42971-7 / J. Biol. Chem. / A sensitive immunoblotting method for measuring protein synthesis initiation factor levels in lysates of Escherichia coli. (1981)
10.1016/S0022-2836(72)80025-1
10.1016/0022-2836(79)90345-0
10.1073/pnas.79.16.4937
10.1111/j.1432-1033.1982.tb06556.x / Eur. J. Biochem. / Expression of the gene for Escherichia coli initiation factor IF-3 in vivo and in vitro. Over-production in recombinant-plasmid-bearing strains. (1982)
Lowary, P., J. Sampson, J. Milligan, D. Groebe, and O.C. Uhlenbeck. 1986. A better way to make RNA for physical studies. In Structure and dynamics of RNA (ed. P.H. van Knippenberg and C.W. Hilbers), pp. 69–76. Plenum Press, New York. (10.1007/978-1-4684-5173-3_6)
Maniatis, T., E.F. Fritsch, and J. Sambrook. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
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10.1111/j.1432-1033.1976.tb10726.x / Eur. J. Biochem. / Specificity and properties of the destabilization, induced by initiation factor IF-3, of ternary complexes of the 30-S ribosomal subunit, aminoacyl-tRNA and polynucleotides. (1976)
10.1016/S0022-2836(83)80036-9
10.1002/j.1460-2075.1982.tb01166.x / EMBO J. / Sequence of a 1.26 kb DNA fragment containing the structural gene for E. coli initiation factor IF3: Presence of an AUU initiator codon. (1982)
10.1128/jb.171.7.3901-3908.1989 / J. Bacteriol. / Ribosome binding sites and RNA processing sites in the transcript of the unc operon of Escherichia coli. (1989)
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10.1016/S0021-9258(18)91930-7 / J. Biol. Chem. / Characterization of two species of methionine transfer ribonucleic acid from bakers' yeast. (1968)
{'key': '2021111320121084000_4.10.1790.39', 'first-page': '3340', 'article-title': 'Role of the constant uridine in binding of yeast tRNAPhe anticodon arm to 30S ribosomes.', 'volume': '10', 'year': '1982', 'journal-title': 'Nucleic Acids Res.'} / Nucleic Acids Res. / Role of the constant uridine in binding of yeast tRNAPhe anticodon arm to 30S ribosomes. (1982)
10.1093/nar/11.7.2035
10.1073/pnas.84.22.7822
10.1021/bi00272a025
10.1073/pnas.76.7.3289

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	1 year, 6 months ago (Feb. 14, 2024, 4:27 p.m.)
Indexed	2 months ago (July 2, 2025, 2:46 p.m.)
Issued	34 years, 11 months ago (Oct. 1, 1990)
Published	34 years, 11 months ago (Oct. 1, 1990)
Published Online	34 years, 11 months ago (Oct. 1, 1990)
Published Print	34 years, 11 months ago (Oct. 1, 1990)

Funders 0

None

BibTeX

@article{Hartz_1990, title={Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3.}, volume={4}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.4.10.1790}, DOI={10.1101/gad.4.10.1790}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Hartz, D and Binkley, J and Hollingsworth, T and Gold, L}, year={1990}, month=oct, pages={1790–1800} }

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