DOI: 10.1101/gad.3.7.936. The yeast transcription activator PRTF, a homolog of the mammalian serum response factor, is encoded by the MCM1 gene.

The yeast transcription activator PRTF, a homolog of the mammalian serum response factor, is encoded by the MCM1 gene.

10.1101/gad.3.7.936

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Two proteins, alpha 1 and pheromone/receptor transcription factor (PRTF), bind cooperatively to the upstream activation sequences (UAS) of yeast alpha-specific genes and thereby activate their transcription. In these protein-DNA complexes, the PRTF moiety interacts with a degenerate dyad symmetric sequence, the P box. PRTF contributes also to the regulation of a second set of cell-type-specific genes, the a-specific genes. We used two in vitro assays to show that PRTF is encoded, at least in part, by the MCM1 gene. In one assay, truncated MCM1 proteins encoded by deletion derivatives of the MCM1 gene formed protein-DNA complexes of novel mobility, demonstrated that MCM1 can bind to the P-box-containing DNA. Second, antibodies raised to a synthetic MCM1 polypeptide retard the migration of PRTF-DNA complexes in gel mobility shift assays. This result indicates that PRTF, defined as an activity that binds cooperatively with alpha 1 to alpha-specific UAS elements, shares an epitope with MCM1. In addition, we show that MCM1 deletions that remove the carboxy-terminal 129 codons of 286 total codons encode truncated MCM1 molecules that are competent to activate transcription in vivo, indicating that the carboxy-terminal residues are not required for this process.

Bibliography

Jarvis, E. E., Clark, K. L., & Sprague, G. F. (1989). The yeast transcription activator PRTF, a homolog of the mammalian serum response factor, is encoded by the MCM1 gene. Genes & Development, 3(7), 936â945.

Authors 3

E E Jarvis (first)
K L Clark (additional)
G F Sprague (additional)

References 37 Referenced 114

10.1038/275104a0
10.1016/0092-8674(87)90326-6
10.1016/0378-1119(79)90004-0 / Gene / Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments. (1979)
10.1016/0003-2697(81)90281-5
10.1128/jb.143.2.971-980.1980 / J. Bacteriol. / In vitro gene fusions that join an enzymatically active β-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. (1980)
10.1016/0092-8674(88)90144-4
10.1007/BF00331501
10.1016/0378-1119(87)90286-1
10.1093/genetics/121.2.223 / Genetics / The MFα1 gene of Saccharomyces cerevisiae: Genetic mapping and mutational analysis of promoter elements. (1989)
10.1093/nar/9.23.6505
10.1073/pnas.81.20.6442
10.1101/gad.2.12b.1713
10.1128/mr.52.4.536-553.1988 / Microbiol. Rev. / Life cycle of the budding yeast Saccharomyces cerevisiae. (1988)
10.1016/0092-8674(86)90070-X
{'key': '2021111320002564000_3.7.936.15', 'first-page': '3185', 'article-title': 'Identification of sequence elements that confer cell-type-specific control of MFα1 expression in Saccharomyces cerevisiae.', 'volume': '7', 'year': '1987', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Identification of sequence elements that confer cell-type-specific control of MFα1 expression in Saccharomyces cerevisiae. (1987)
{'key': '2021111320002564000_3.7.936.16', 'first-page': '309', 'article-title': 'Identification of a DNA segment that is necessary and sufficient for α-specific gene control in Saccharomyces cerevisiae: Implications for regulation of α-specific and a-specific genes.', 'volume': '8', 'year': '1988', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Identification of a DNA segment that is necessary and sufficient for α-specific gene control in Saccharomyces cerevisiae: Implications for regulation of α-specific and a-specific genes. (1988)
10.1016/S0092-8674(85)80119-7
Kagen, A. and M. Glick. 1979. Methods of radioimmunoassay (ed. X. Jaffe and X. Behrman), pp. 328–329. Academic Press, New York.
10.1126/science.3080805
10.1016/S0092-8674(88)90449-7
10.1016/0092-8674(83)90410-5
10.1016/0092-8674(87)90491-0
10.1038/227680a0
10.1093/genetics/106.3.365 / Genetics / Mutants of S. cerevisiae defective in the maintenance of minichromosomes. (1984)
Maniatis, T., E.F. Fritsch, and J. Sambrook. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Miller, J.H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
10.1126/science.3306917
10.1016/0092-8674(88)90244-9
10.1016/0022-2836(88)90358-0
10.1016/0092-8674(89)90903-3
10.1073/pnas.83.10.3248
10.1073/pnas.74.12.5463
Sherman, F., G.R. Fink, and J.B. Hicks. 1982. Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
10.1146/annurev.mi.37.100183.003203
10.1002/j.1460-2075.1988.tb03323.x / EMBO J. / Interactions of purified transcription factors: Binding of yeast MATα1 and PRTF to cell type-specific, upstream activating sequences. (1988)
10.1073/pnas.76.9.4350
10.1016/0378-1119(85)90120-9

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:02 p.m.)
Indexed	1 year, 6 months ago (Feb. 25, 2024, 11:01 p.m.)
Issued	36 years, 1 month ago (July 1, 1989)
Published	36 years, 1 month ago (July 1, 1989)
Published Online	36 years, 1 month ago (July 1, 1989)
Published Print	36 years, 1 month ago (July 1, 1989)

Funders 0

None

BibTeX

@article{Jarvis_1989, title={The yeast transcription activator PRTF, a homolog of the mammalian serum response factor, is encoded by the MCM1 gene.}, volume={3}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.3.7.936}, DOI={10.1101/gad.3.7.936}, number={7}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Jarvis, E E and Clark, K L and Sprague, G F}, year={1989}, month=jul, pages={936–945} }

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