A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes.
10.1101/gad.3.7.921
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

The Saccharomyces cerevisiae MCM1 gene product is a protein with multiple functions. It is a transcription factor necessary for expression of mating-type-specific genes and is also required for the maintenance of minichromosomes. MCM1 shows DNA-binding specificities similar to those of two previously reported DNA-binding factors, pheromone/receptor transcription factor (PRTF) and general regulator of mating type (GRM); like PRTF, its activity can be modulated by the alpha 1 protein. MCM1 binds to the dyad symmetry element 5'-CCTAATTAGG and related sequences, which we refer to as MCM1 control elements (MCEs). MCEs are found within the regulatory regions of a- and alpha-specific genes. Direct and indirect DNA binding assays suggest that a conserved 5'-ATTAGG in one-half of the dyad symmetry element is important for MCM1 binding whereas variants in the other half are tolerated. We have used a novel DNase I 'nicking interference' assay to investigate the interaction of MCM1 with its substrate. These data suggest that MCM1 binds as a dimer, interacting symmetrically with the ATTAGG residues in each half of the binding site. MCM1 contains striking homology to the DNA-binding domain of the human serum response factor (SRF) which mediates the transient transcriptional activation of growth-stimulated genes by binding to the serum response element (SRE). We have shown that MCM1 binds to the human c-fos SRE in vitro and, like other MCEs, the c-fos SRE exhibits MCM1-mediated upstream activating sequence (UAS) activity in vivo.

Bibliography

Passmore, S., Elble, R., & Tye, B. K. (1989). A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes. Genes & Development, 3(7), 921–935.

Authors 3
  1. S Passmore (first)
  2. R Elble (additional)
  3. B K Tye (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 11:02 p.m.)
Indexed 11 months, 1 week ago (Sept. 12, 2024, 10:50 p.m.)
Issued 36 years, 1 month ago (July 1, 1989)
Published 36 years, 1 month ago (July 1, 1989)
Published Online 36 years, 1 month ago (July 1, 1989)
Published Print 36 years, 1 month ago (July 1, 1989)
Funders 0

None

@article{Passmore_1989, title={A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes.}, volume={3}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.3.7.921}, DOI={10.1101/gad.3.7.921}, number={7}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Passmore, S and Elble, R and Tye, B K}, year={1989}, month=jul, pages={921–935} }