DOI: 10.1101/gad.3.5.620. DNA-binding activity of the adenovirus-induced E4F transcription factor is regulated by phosphorylation.

DNA-binding activity of the adenovirus-induced E4F transcription factor is regulated by phosphorylation.

10.1101/gad.3.5.620

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Previous experiments have identified E4F, an inducible cellular factor that binds to sequences in the adenovirus E4 promoter that are critical for E1A-dependent transcriptional activation. The E4F factor has been purified and shown to stimulate transcription in vitro from the E4 promoter. Analysis of the affinity-purified factor identifies a single polypeptide of 50 kD that has E4F-specific binding activity. E4F binding activity is also regulated during F9 cell differentiation and can be activated in differentiated F9 cells by viral infection. Furthermore, the activation process appears to involve a phosphorylation event, because treatment of E4F with alkaline phosphatase abolishes activity and incubation of the phosphatase-inactivated factor with an extract from virus infected cells restores activity.

Bibliography

Raychaudhuri, P., Bagchi, S., & Nevins, J. R. (1989). DNA-binding activity of the adenovirus-induced E4F transcription factor is regulated by phosphorylation. Genes & Development, 3(5), 620â627.

Authors 3

P Raychaudhuri (first)
S Bagchi (additional)
J R Nevins (additional)

References 37 Referenced 73

Bagchi, S., Raychaudhuri, P. and Nevins. J.R. 1989. Phosphorylation-dependent activation of the adenovirus inducible E2F transcription factor in a cell-free system. Proc. Natl. Acad. Sci. (in press). (10.1073/pnas.86.12.4352)
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{'key': '2021111319552320000_3.5.620.5', 'first-page': '2653', 'article-title': 'E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.', 'volume': '5', 'year': '1985', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA. (1985)
10.1126/science.2996135
10.1093/nar/12.20.7877
10.1016/0003-2697(80)90013-5
10.1101/gad.2.10.1216
10.1126/science.3291115
10.1016/S0092-8674(85)80076-3
10.1016/S0092-8674(88)90409-6
10.1101/gad.1.10.1132
{'key': '2021111319552320000_3.5.620.14', 'first-page': '867', 'article-title': 'Common control of the heat shock gene and early adenovirus genes: Evidence for a cellular E1A-like activity.', 'volume': '4', 'year': '1984', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Common control of the heat shock gene and early adenovirus genes: Evidence for a cellular E1A-like activity. (1984)
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10.1002/j.1460-2075.1987.tb02374.x / EMBO J. / A cellular transcription factor E4F1 interacts with an E1A-inducible enhancer and mediates constitutive enhancer function in vitro. (1987)
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10.1016/S0076-6879(80)65072-1 / Methods Enzymol. / Definition and mapping of adenovirus-2 nuclear transcription. (1980)
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10.1128/mr.51.4.419-430.1987 / Microbiol. Rev. / Regulation of early adenovirus gene expression. (1987)
10.1128/jvi.32.3.727-733.1979 / J. Virol. / Regulation of the primary expression of the early adenovirus transcription units. (1979)
10.1073/pnas.85.19.7206
10.1002/j.1460-2075.1987.tb02753.x / EMBO J. / Identification of an E1A-inducible cellular factor that interacts with regulatory sequences within the adenovirus E4 promoter. (1987)
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{'key': '2021111319552320000_3.5.620.33', 'first-page': '81', 'article-title': 'Heat shock factor is regulated differently in yeast and HeLa cells.', 'volume': '326', 'year': '1987', 'journal-title': 'Nature'} / Nature / Heat shock factor is regulated differently in yeast and HeLa cells. (1987)
10.1038/326512a0
10.1038/334494a0
{'key': '2021111319552320000_3.5.620.36', 'first-page': '578', 'article-title': 'The adenovirus-inducible factor E2F stimulates transcription after specific DNA-binding.', 'volume': '9', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The adenovirus-inducible factor E2F stimulates transcription after specific DNA-binding. (1989)
10.1002/j.1460-2075.1986.tb04218.x / EMBO J. / Adenovirus stimulation of transcription by RNA polymerase III: Evidence for an E1A-dependent increase in transcription factor IIIC concentration. (1986)

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 10:56 p.m.)
Indexed	1 year, 7 months ago (Jan. 9, 2024, 6:25 p.m.)
Issued	36 years, 3 months ago (May 1, 1989)
Published	36 years, 3 months ago (May 1, 1989)
Published Online	36 years, 3 months ago (May 1, 1989)
Published Print	36 years, 3 months ago (May 1, 1989)

Funders 0

None

BibTeX

@article{Raychaudhuri_1989, title={DNA-binding activity of the adenovirus-induced E4F transcription factor is regulated by phosphorylation.}, volume={3}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.3.5.620}, DOI={10.1101/gad.3.5.620}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Raychaudhuri, P and Bagchi, S and Nevins, J R}, year={1989}, month=may, pages={620–627} }

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