DOI: 10.1101/gad.3.5.598. A bacterial environmental sensor that functions as a protein kinase and stimulates transcriptional activation.

A bacterial environmental sensor that functions as a protein kinase and stimulates transcriptional activation.

10.1101/gad.3.5.598

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

Transcription of the genes that encode the major outer membrane porin proteins OmpF and OmpC of Escherichia coli is regulated in response to changes in medium osmolarity by EnvZ and OmpR. EnvZ functions to sense environmental conditions and to relay this information to the DNA-binding protein OmpR. We have used a truncated EnvZ protein (EnvZ115), which is defective in sensory function but able to communicate with OmpR, to study the biochemical interactions between these two proteins and their effects on transcription from the ompF promoter. We show that purified EnvZ115 can phosphorylate OmpR in the presence of ATP. In addition, EnvZ115 stimulates the ability of OmpR to activate ompF transcription in vitro. Using antibodies specific for EnvZ, we have purified the wild-type protein and have shown that it is also an OmpR kinase. These results provide a prokaryotic example of a transmembrane sensory protein that functions as a protein kinase and suggest a mechanism by which EnvZ communicates with OmpR in signal transduction.

Bibliography

Igo, M. M., Ninfa, A. J., & Silhavy, T. J. (1989). A bacterial environmental sensor that functions as a protein kinase and stimulates transcriptional activation. Genes & Development, 3(5), 598â605.

Authors 3

M M Igo (first)
A J Ninfa (additional)
T J Silhavy (additional)

References 33 Referenced 123

10.1007/BF02424561
10.1038/285167a0
10.1084/jem.99.2.167
10.1016/0092-8674(84)90091-6
10.1016/S0021-9258(18)49274-5 / J. Biol. Chem. / Localization and membrane topology of EnvZ, a protein involved in osmoregulation of OmpF and OmpC in Escherichia coli. (1987)
Garrett, S. 1986. Ph.D dissertation, “Genetic studies on the synthesis of the major outermembrane porin proteins of E. coli K12.” The Johns Hopkins University, Baltimore, Maryland.
10.1128/jb.156.1.62-69.1983 / J. Bacteriol. / Isolation and characterization of chain-terminating nonsense mutations in a porin regulator gene, envZ. (1983)
10.1128/jb.140.2.342-350.1979 / J. Bacteriol. / Transcriptional regulation of Escherichia coli K-12 major outer membrane protein 1b. (1979)
10.1016/0022-2836(81)90364-8
10.1038/336139a0
10.1016/0092-8674(88)90489-8
10.1128/jb.170.12.5971-5973.1988 / J. Bacteriol. / EnvZ, a transmembrane environmental sensor of Escherichia coli K-12 is phosphorylated in vitro. (1988)
10.1016/S0021-9258(18)66860-7 / J. Biol. Chem. / Purification and characterization of the OmpR protein, a positive regulator involved in osmoregulatory expression of the ompF and ompC genes in Escherichia coli. (1986)
10.1128/jb.140.3.843-847.1979 / J. Bacteriol. / Influence of molecular size and osmolarity of sugars and dextrans on the synthesis of outer membrane proteins O-8 and O-9 of Escherichia coli K-12. (1979)
10.1073/pnas.85.14.4976
{'key': '2021111319552319000_3.5.598.16', 'first-page': '14629', 'article-title': 'Activation of the ompC gene by the OmpR protein of Escherichia coli.', 'volume': '263', 'year': '1988', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Activation of the ompC gene by the OmpR protein of Escherichia coli. (1988)
10.1016/S0021-9258(19)35453-5 / J. Biol. Chem. / Interaction of OmpR, a positive regulator, with the osmoregulated ompC and ompF genes of Escherichia coli. (1988)
10.1073/pnas.83.16.5909
Ninfa, A.J., E. Brodsky, and B. Magasanik. 1989. Role of NRI-phosphate in the activation of transcription initiation from the nitrogen regulated promoter glnAp2. In DNA-protein interactions in transcription (ed. J. Gralla), pp. 43–52. UCLA Symp. on Mol. and Cell Biology. Vol 95. Alan R. Liss, New York.
10.1073/pnas.85.15.5492
10.1016/S0021-9258(19)76006-2 / J. Biol. Chem. / Interaction of a transcriptional activator, OmpR, with reciprocally osmoregulated genes, ompF and ompC, of Escherichia coli. (1986)
10.1128/jb.155.1.265-274.1983 / J. Bacteriol. / Interactions between chemotaxis genes and flagellar genes in Escherichia coli. (1983)
10.1016/0092-8674(87)90530-7
Silhavy, T.J., M.L. Berman, and L.W. Enquist. 1984. Experiments in gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
10.1128/jb.170.1.439-441.1988 / J. Bacteriol. / EnvZ functions through OmpR to control porin gene expression in Escherichia coli K-12. (1988)
10.1016/S0021-9258(18)66832-2 / J. Biol. Chem. / Kinetic analysis of 1amB mutants suggests the signal sequence plays multiple roles in protein export. (1986)
10.1128/jb.170.12.5728-5738.1988 / J. Bacteriol. / N-terminal half of CheB is involved in methylesterase response to negative chemotactic stimuli in Escherichia coli. (1988)
Stock, A.M., Wylie, D.C. Mottenen, J.M. Lupas, A.N. Ninfa, E.G. Ninfa, A.J. Schutt, C.E. and Stock. J.B. 1989. Phospho-proteins involved in bacterial signal transduction. Cold Spring Harbor Symp. Quant. Biol. (in press). (10.1101/SQB.1988.053.01.009)
10.1016/S0022-2836(83)80085-0
10.1128/jb.131.2.623-630.1977 / J. Bacteriol. / Influence of osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli. (1977)
10.1073/pnas.80.14.4432
10.1073/pnas.85.23.8919
10.1016/S0006-291X(88)80365-6

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 10:57 p.m.)
Indexed	1 year ago (Aug. 11, 2024, 3:09 p.m.)
Issued	36 years, 3 months ago (May 1, 1989)
Published	36 years, 3 months ago (May 1, 1989)
Published Online	36 years, 3 months ago (May 1, 1989)
Published Print	36 years, 3 months ago (May 1, 1989)

Funders 0

None

BibTeX

@article{Igo_1989, title={A bacterial environmental sensor that functions as a protein kinase and stimulates transcriptional activation.}, volume={3}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.3.5.598}, DOI={10.1101/gad.3.5.598}, number={5}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Igo, M M and Ninfa, A J and Silhavy, T J}, year={1989}, month=may, pages={598–605} }

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