The Hox-1.3 homeo box protein is a sequence-specific DNA-binding phosphoprotein.
10.1101/gad.3.2.158
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

We report that the murine Hox-1.3 homeo domain protein is a nuclear phosphoprotein capable of binding to specific DNA sequences. DNase I protection of the Hox-1.3 gene promoter region with the Hox-1.3 protein identifies a binding site 144 bp upstream from the start of transcription. Both phosphorylated and nonphosphorylated forms bind DNA directly in a sequence-specific manner. Electrophoretic mobility shift assays were performed with a set of synthetic oligonucleotides representing either the DNase I-protected region of the Hox-1.3 gene or partially homologous sequences present in promoter regions of other characterized viral, yeast, and mammalian genes. From the results, we deduce a consensus binding motif of CPyPyNATTAT/GPy. Base substitutions in the core ATTA sequence severely reduce or abolish binding. In the SV40 enhancer, the Hox-1.3 binding motif overlaps both the octamer (Octa2) and the transactivator protein-1 (AP-1) binding sites. The Hox-1.3 binding motif also overlaps the nuclear factor III (NF-III) octamer motif in the adenovirus-2 origin of DNA replication. Overlap among DNA-binding sites suggests that regulation imparted by certain cis-elements may be integrated by these different factors.

Bibliography

Odenwald, W. F., Garbern, J., Arnheiter, H., Tournier-Lasserve, E., & Lazzarini, R. A. (1989). The Hox-1.3 homeo box protein is a sequence-specific DNA-binding phosphoprotein. Genes & Development, 3(2), 158–172.

Authors 5
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  2. J Garbern (additional)
  3. H Arnheiter (additional)
  4. E Tournier-Lasserve (additional)
  5. R A Lazzarini (additional)
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Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 10:51 p.m.)
Indexed 1 month, 3 weeks ago (July 1, 2025, 9:31 a.m.)
Issued 36 years, 6 months ago (Feb. 1, 1989)
Published 36 years, 6 months ago (Feb. 1, 1989)
Published Online 36 years, 6 months ago (Feb. 1, 1989)
Published Print 36 years, 6 months ago (Feb. 1, 1989)
Funders 0

None

@article{Odenwald_1989, title={The Hox-1.3 homeo box protein is a sequence-specific DNA-binding phosphoprotein.}, volume={3}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.3.2.158}, DOI={10.1101/gad.3.2.158}, number={2}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Odenwald, W F and Garbern, J and Arnheiter, H and Tournier-Lasserve, E and Lazzarini, R A}, year={1989}, month=feb, pages={158–172} }