DOI: 10.1101/gad.3.2.141. Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.

Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.

10.1101/gad.3.2.141

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

During sporulation of Bacillus subtilis, two identical genomes segregate in two compartments, the forespore and mother cell. These genomes are expressed differentially, with some genes such as sspE turned on only in the forespore. In vitro transcription of sspE was obtained only with RNA polymerase extracted from sporulating cells. Fractionation of factors associated with this enzyme and reconstitution with core RNA polymerase from vegetative cells generated an enzyme accurately transcribing sspE in vitro and led to purification of a polypeptide with the amino-terminal sequence of the spoIIIG product. Inactivation of spoIIIG abolished expression of sspE and five other forespore-specific genes, whereas synthesis of the spoIIIG product in vegetative cells rapidly turned these genes on. Therefore, spoIIIG encodes a sigma-factor, sigma G, which controls the expression of multiple genes in the forespore compartment.

Bibliography

Sun, D. X., Stragier, P., & Setlow, P. (1989). Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis. Genes & Development, 3(2), 141â149.

Authors 3

D X Sun (first)
P Stragier (additional)
P Setlow (additional)

References 30 Referenced 157

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10.1038/227680a0
10.1128/jb.167.1.168-173.1986 / J. Bacteriol. / Cloning and nucleotide sequencing of genes for small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus: and ‘Thermoactinomyces thalpophilus’. (1986)
10.1038/227910a0
10.1146/annurev.ge.20.120186.003205
10.1093/nar/16.14.6567
10.1128/jb.170.1.239-244.1988 / J. Bacteriol. / Studies on the regulation of expression of genes coding for small, acid-soluble proteins of Bacillus subtilis spores using lacZ gene fusions. (1988)
10.1016/S0021-9258(18)61070-1 / J. Biol. Chem. / Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. (1987)
10.1126/science.6162199
10.1016/0003-2697(77)90020-3
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10.1016/S0021-9258(19)43523-0 / J. Biol. Chem. / Comparative size and properties of the sigma subunits of ribonucleic acid polymerase from Bacillus subtilis and Escherichia coli. (1973)
Stragier, P. 1986. Comment on ‘Duplicated sporulation genes in bacteria’ by J. Errington, P. Fort, and J. Mandelstam. FEBS Lett. 188 (1984-188, 1985), FEBS Lett. 195: 9–11. (10.1016/0014-5793(86)80119-3)
10.1016/0092-8674(88)90407-2
10.1016/0092-8674(83)90544-5
Zuber, P., Healy, J. Carter, H.L. III, Cutting, S. Moran C.P. Jr., and Losick. R. 1989. Mutation changing the specificity of an RNA polymerase sigma factor. J. Mol. Biol., (in press). (10.1016/0022-2836(89)90569-X)

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 10:50 p.m.)
Indexed	1 year ago (Aug. 3, 2024, 2:53 p.m.)
Issued	36 years, 7 months ago (Feb. 1, 1989)
Published	36 years, 7 months ago (Feb. 1, 1989)
Published Online	36 years, 7 months ago (Feb. 1, 1989)
Published Print	36 years, 7 months ago (Feb. 1, 1989)

Funders 0

None

BibTeX

@article{Sun_1989, title={Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.}, volume={3}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.3.2.141}, DOI={10.1101/gad.3.2.141}, number={2}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Sun, D X and Stragier, P and Setlow, P}, year={1989}, month=feb, pages={141–149} }

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