DOI: 10.1101/gad.3.10.1613. The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA.

The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA.

10.1101/gad.3.10.1613

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

The cognate sequence of transcription factor PTF1, which plays a key role in pancreas-specific gene expression, has a bipartite organization. Two separate DNA domains, the A and the B boxes, are required for efficient binding of the factor. The structure of PTF1 was elucidated by cross-linking purified PTF1 to DNA templates that had been differentially substituted with azido-deoxyuridine (N3.dU). This site-directed UV cross-linking shows that PTF1 contains two DNA-binding proteins, distinct in size and sensitivity to Staphylococcus aureus V8 protease. A 64-kD protein is cross-linked with DNA containing N3.dU substitutions in the A box, and a 48-kD protein is cross-linked with DNA containing N3.dU substitutions in the B box. Both proteins bind simultaneously to the same DNA molecule. The data indicate that PTF1 is a heteromeric oligomer and that its cell-specific DNA-binding potential is the result of a concerted activity of two DNA-binding subunits.

Bibliography

Roux, E., Strubin, M., HagenbÃ¼chle, O., & Wellauer, P. K. (1989). The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA. Genes & Development, 3(10), 1613â1624.

Authors 4

E Roux (first)
M Strubin (additional)
O Hagenbüchle (additional)
P K Wellauer (additional)

References 18 Referenced 59

10.1073/pnas.83.11.3599
10.1016/0092-8674(87)90036-5
{'key': '2021111320013047000_3.10.1613.3', 'first-page': '4723', 'article-title': 'A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.', 'volume': '6', 'year': '1986', 'journal-title': 'Mol Cell. Biol.'} / Mol Cell. Biol. / A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor. (1986)
10.1016/0092-8674(88)90484-9
{'key': '2021111320013047000_3.10.1613.5', 'first-page': '2464', 'article-title': 'Identification of a cell-specific DNA-binding activity that interacts with a transcriptional activator of genes expressed in the acinar pancreas.', 'volume': '9', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Identification of a cell-specific DNA-binding activity that interacts with a transcriptional activator of genes expressed in the acinar pancreas. (1989)
10.1093/nar/11.5.1475
10.1021/bi00375a037
10.1126/science.2832951
10.1016/0092-8674(88)90147-X
10.1002/j.1460-2075.1988.tb03000.x / EMBO J. / Two distinct factors interact with the promoter regions of several liver specific genes. (1988)
10.1016/0092-8674(86)90070-X
{'key': '2021111320013047000_3.10.1613.12', 'first-page': '212', 'article-title': 'Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors.', 'volume': '16', 'year': '1980', 'journal-title': 'In Vitro'} / In Vitro / Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors. (1980)
{'key': '2021111320013047000_3.10.1613.13', 'first-page': '893', 'article-title': 'The cell-specific elastase I enhancer comprises two domains.', 'volume': '13', 'year': '1988', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / The cell-specific elastase I enhancer comprises two domains. (1988)
10.1038/227680a0
10.1016/0092-8674(87)90583-6
10.1016/0092-8674(88)90146-8
10.1016/0092-8674(87)90582-4
10.1016/0092-8674(88)90059-1

Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:02 p.m.)
Indexed	11 months, 4 weeks ago (Sept. 5, 2024, 1:52 p.m.)
Issued	35 years, 11 months ago (Oct. 1, 1989)
Published	35 years, 11 months ago (Oct. 1, 1989)
Published Online	35 years, 11 months ago (Oct. 1, 1989)
Published Print	35 years, 11 months ago (Oct. 1, 1989)

Funders 0

None

BibTeX

@article{Roux_1989, title={The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA.}, volume={3}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.3.10.1613}, DOI={10.1101/gad.3.10.1613}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Roux, E and Strubin, M and Hagenbüchle, O and Wellauer, P K}, year={1989}, month=oct, pages={1613–1624} }

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