DOI: 10.1101/gad.3.10.1518. Spatial regulation of zerknüllt: a dorsal-ventral patterning gene in Drosophila.

Spatial regulation of zerknüllt: a dorsal-ventral patterning gene in Drosophila.

10.1101/gad.3.10.1518

Crossref journal-article

Cold Spring Harbor Laboratory

Genes & Development (246)

Abstract

zerknüllt (zen) is one of approximately 10 zygotically active genes that control the differentiation of the dorsal-ventral (D/V) pattern during early embryogenesis in Drosophila. Past genetic analyses suggest that maternal factors repress the expression of zen in ventral regions, thereby restricting zen products to dorsal and dorsal-lateral regions of precellular embryos. Subsequent interactions with other zygotic D/V regulatory genes refine the zen pattern, restricting expression to the dorsal-most ectoderm. Here we describe the use of zen promoter fusions and P-element transformation to identify cis elements that are responsible for the complex spatial pattern of zen expression. The zen promoter shows a two-tier organization: Distal sequences mediate its initial response to maternal factors, whereas proximal sequences are responsible for the refinement of the pattern in older embryos. The distal regulatory element has the property of a silencer (or anti-enhancer) element and can act over a distance to repress ventral expression of a heterologous promoter. Also, we discuss evidence that proximal promoter sequences interact with factors that may be modulated by a cell-cell communication pathway.

Bibliography

Doyle, H. J., Kraut, R., & Levine, M. (1989). Spatial regulation of zerknÃ¼llt: a dorsal-ventral patterning gene in Drosophila. Genes & Development, 3(10), 1518â1533.

Authors 3

H J Doyle (first)
R Kraut (additional)
M Levine (additional)

References 51 Referenced 87

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10.1093/genetics/108.4.953 / Genetics / Tissue-specific and pretranslational character of variants of the rosy locus control element in Drosophila melanogaster. (1984)
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{'key': '2021111320013043000_3.10.1518.14', 'first-page': '349', 'article-title': 'The v-rel oncogene encodes a cell-specific transcriptional activator of certain promoters.', 'volume': '3', 'year': '1988', 'journal-title': 'Oncogene'} / Oncogene / The v-rel oncogene encodes a cell-specific transcriptional activator of certain promoters. (1988)
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{'key': '2021111320013043000_3.10.1518.23', 'first-page': '493', 'article-title': 'Transcription pattern of the Drosophila segmentation gene hairy.', 'volume': '318', 'year': '1985', 'journal-title': 'Nature'} / Nature / Transcription pattern of the Drosophila segmentation gene hairy. (1985)
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10.1093/genetics/116.1.67 / Genetics / Sequence of the structural gene for xanthine dehydrogenase (rosy locus) in Drosophila melanogaster. (1987)
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10.1093/genetics/116.1.55 / Genetics / Mutations affecting expression of the ry locus in Drosophila melanogaster. (1987)
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Rushlow, C. and M. Levine. 1989. The role of the zerknüllt gene in dorsal-ventral pattern formation in Drosophila. In Advances in genetics (in press). (10.1016/S0065-2660(08)60028-0)
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Schröder, C., Tautz, D. Seifert, F. and Jäckle. H. Differential regulation of the two transcripts from the Drosophila gap segmentation gene hunchback. EMBO J. 7: 2881–2887. (10.1002/j.1460-2075.1988.tb03145.x)
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Dates

Type	When
Created	18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited	3 years, 9 months ago (Nov. 13, 2021, 11:03 p.m.)
Indexed	3 weeks, 4 days ago (July 31, 2025, 11:47 p.m.)
Issued	35 years, 10 months ago (Oct. 1, 1989)
Published	35 years, 10 months ago (Oct. 1, 1989)
Published Online	35 years, 10 months ago (Oct. 1, 1989)
Published Print	35 years, 10 months ago (Oct. 1, 1989)

Funders 0

None

BibTeX

@article{Doyle_1989, title={Spatial regulation of zerknüllt: a dorsal-ventral patterning gene in Drosophila.}, volume={3}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.3.10.1518}, DOI={10.1101/gad.3.10.1518}, number={10}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Doyle, H J and Kraut, R and Levine, M}, year={1989}, month=oct, pages={1518–1533} }

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