A new look into an old problem: keratins as tools to investigate determination, morphogenesis, and differentiation in skin.
10.1101/gad.3.1.1
Crossref journal-article
Cold Spring Harbor Laboratory
Genes & Development (246)
Abstract

We have investigated keratin and keratin mRNA expression during (1) differentiation of stem cells into epidermis and hair follicles and (2) morphogenesis of follicles. Our results indicate that a type I keratin K14 is expressed early in embryonal basal cells. Subsequently, its expression is elevated in the basal layer of developing epidermis but suppressed in developing matrix cells. This difference represents an early and major biochemical distinction between the two diverging cell types. Moreover, because expression of this keratin is not readily influenced by extracellular regulators or cell culture, it suggests a well-defined and narrow window of development during which an irreversible divergence in basal and matrix cells may take place. In contrast to K14, which is expressed very early in development and coincident with basal epidermal differentiation, a hair-specific type I keratin and its mRNA is expressed late in hair matrix development and well after follicle morphogenesis. Besides providing an additional developmental difference between epidermal and hair matrix cells, the hair-specific keratins provide the first demonstration that keratin expression may be a consequence rather than a cause of cell organization and differentiation.

Bibliography

Kopan, R., & Fuchs, E. (1989). A new look into an old problem: keratins as tools to investigate determination, morphogenesis, and differentiation in skin. Genes & Development, 3(1), 1–15.

Authors 2
  1. R Kopan (first)
  2. E Fuchs (additional)
References 71 Referenced 120
  1. 10.1083/jcb.93.3.551
  2. Billingham, R.E. and W.K. Silvers 1968. Dermo-epidermal interactions and epithelial specificity. Epithelial-mesenchymal interactions (ed. R. Fleischmajer and R.E. Billingham), pp 252. Williams and Wilkins Co., Baltimore.
  3. {'key': '2021111319494807000_3.1.1.3', 'first-page': '511', 'article-title': 'Fine structure of the early hair germ and dermal papilla in the human foetus.', 'volume': '102', 'year': '1968', 'journal-title': 'J. Anat.'} / J. Anat. / Fine structure of the early hair germ and dermal papilla in the human foetus. (1968)
  4. {'key': '2021111319494807000_3.1.1.4', 'first-page': '117', 'article-title': 'The tranplantation of individual rat and guinea-pig whisker papillae.', 'volume': '9', 'year': '1961', 'journal-title': 'J. Embryol. Exp. Morphol.'} / J. Embryol. Exp. Morphol. / The tranplantation of individual rat and guinea-pig whisker papillae. (1961)
  5. 10.1016/0012-1606(84)90162-3
  6. 10.1016/0141-8130(83)90040-5
  7. 10.1083/jcb.101.4.1257
  8. {'key': '2021111319494807000_3.1.1.8', 'first-page': '342', 'article-title': 'The development of mouse vibrissae in vivo and in vitro.', 'volume': '86', 'year': '1952', 'journal-title': 'J. Anat. Physiol.'} / J. Anat. Physiol. / The development of mouse vibrissae in vivo and in vitro. (1952)
  9. 10.1007/BF00857264
  10. 10.1016/0092-8674(80)90150-6
  11. 10.1042/bj2360695
  12. 10.1083/jcb.98.4.1388
  13. 10.1083/jcb.102.4.1412
  14. 10.1016/0092-8674(80)90094-X
  15. 10.1016/0092-8674(81)90169-0
  16. 10.1073/pnas.80.19.5857
  17. 10.1016/S0070-2153(08)60097-6 / Curr. Top. Dev. Biol. / The human keratin genes and their differential expression. (1987)
  18. 10.1016/0092-8674(82)90424-X
  19. 10.1016/0092-8674(83)90034-X
  20. 10.1007/BF00525727
  21. 10.1111/j.1432-0436.1988.tb00805.x
  22. 10.1111/j.1432-0436.1988.tb00724.x
  23. 10.1111/j.1432-0436.1986.tb00562.x
  24. 10.1016/0092-8674(80)90406-7
  25. 10.1071/BI9820103 / Aust. J. Biol. Sci. / In vitro growth and differentiation of epithelial cells derived from post-embryonic hair follicles. (1982)
  26. {'key': '2021111319494807000_3.1.1.26', 'first-page': '209', 'article-title': 'A quantitative study of hair growth using mouse and rat vibrissal follicles.', 'volume': '72', 'year': '1982', 'journal-title': 'J. Embryol. Exp. Morph.'} / J. Embryol. Exp. Morph. / A quantitative study of hair growth using mouse and rat vibrissal follicles. (1982)
  27. 10.1111/1523-1747.ep12355183
  28. 10.1111/j.1432-0436.1981.tb01177.x
  29. Jacobson, C.M. 1966. A comparative study of the mechanisms by which x-irradiation and genetic mutation cause loss of vibrissae in embryo mice. J. Embryol. Exp. Morph. 16: 369-179. (10.1242/dev.16.2.369)
  30. 10.1038/311560a0
  31. 10.1111/j.1432-0436.1984.tb00278.x
  32. Jorcano, J.L., Reiger, M. Franz, J.K. Schiller, D.L. Moll, R. and Franke. W.W. 1984b. Identification of two types of keratin polypeptides within the acidic cytokeratin subfamily I. J. Mol. Biol. 179: 357-281. (10.1016/0022-2836(84)90468-6)
  33. 10.1073/pnas.81.14.4280
  34. 10.1111/1523-1747.ep12260492
  35. 10.1083/jcb.105.1.427
  36. 10.1038/227680a0
  37. {'key': '2021111319494807000_3.1.1.37', 'first-page': '486', 'article-title': 'Sequence and expression of a type II keratin, K5, in human epidermal cells.', 'volume': '8', 'year': '1988', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Sequence and expression of a type II keratin, K5, in human epidermal cells. (1988)
  38. 10.1083/jcb.103.6.2593
  39. Maniatis, T., E.F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  40. 10.1071/BI9770389 / Aust. J. Biol. Sci. / The keratin proteins of wool, horn and hoof from sheep. (1977)
  41. {'key': '2021111319494807000_3.1.1.41', 'first-page': '201', 'article-title': 'Development of hair coat and skin glands in fetal porcine integument.', 'volume': '144', 'year': '1986', 'journal-title': 'J. Anat.'} / J. Anat. / Development of hair coat and skin glands in fetal porcine integument. (1986)
  42. 10.1111/j.1432-0436.1982.tb01280.x
  43. 10.1016/0092-8674(82)90400-7
  44. Montagna, W. and P.F. Parakkal. 1974. The structure and function of skin. 3rd ed. Academic Press, New York, pp. 1–433. (10.1016/B978-0-12-505263-4.50006-6)
  45. Moscona, A.A. and B.B. Garber. 1968. Reconstruction of skin from single cells and integumental differentiation in cell aggregates. In Epithelial-mesenchymal interactions. (ed. R.E. Billingham and R.W. Fleischmajer) pp. 230–243. Williams and Wilkins Co., Baltimore.
  46. 10.1177/34.7.2423579
  47. 10.1083/jcb.97.1.244
  48. 10.1016/0092-8674(77)90176-3
  49. {'key': '2021111319494807000_3.1.1.49', 'first-page': '331', 'article-title': 'Whisker growth after removal of the dermal papilla and lengths of follicle in the hooded rat.', 'volume': '15', 'year': '1966', 'journal-title': 'J. Embryol. Exp. Morphol.'} / J. Embryol. Exp. Morphol. / Whisker growth after removal of the dermal papilla and lengths of follicle in the hooded rat. (1966)
  50. {'key': '2021111319494807000_3.1.1.50', 'first-page': '231', 'article-title': 'Histological studies of whisker regeneration in the hooded rat.', 'volume': '16', 'year': '1966', 'journal-title': 'J. Embryol. Exp. Morphol.'} / J. Embryol. Exp. Morphol. / Histological studies of whisker regeneration in the hooded rat. (1966)
  51. {'key': '2021111319494807000_3.1.1.51', 'first-page': '219', 'article-title': 'The induction of hair follicle formation in the adult hooded rat by vibrissa dermal papillae.', 'volume': '23', 'year': '1970', 'journal-title': 'J. Embryol. Exp. Morphol.'} / J. Embryol. Exp. Morphol. / The induction of hair follicle formation in the adult hooded rat by vibrissa dermal papillae. (1970)
  52. Pinkus, F. 1910. The development of the integument. In Manual of human embryology, (ed. Keibel and Mall), vol I, pp. 243–291. Lippincott, Philadelphia.
  53. Pinkus, F. 1958. Embryology of hair. In The biology of hair growth (ed. W. Montagna and R.A. Ellis) pp. 1–23, Academic Press, New York. (10.1016/B978-1-4832-3287-4.50007-6)
  54. {'key': '2021111319494807000_3.1.1.54', 'first-page': '113', 'article-title': 'Induction of hair follicles in mouse skin by rat vibrissa dermal papillae.', 'volume': '94', 'year': '1986', 'journal-title': 'J. Embryol. Exp. Morph.'} / J. Embryol. Exp. Morph. / Induction of hair follicles in mouse skin by rat vibrissa dermal papillae. (1986)
  55. 10.1016/S0092-8674(75)80001-8
  56. 10.1111/1523-1747.ep12471760
  57. 10.1111/j.1432-0436.1987.tb00174.x
  58. 10.1111/1523-1747.ep12291902
  59. 10.1038/302794a0
  60. 10.1073/pnas.81.18.5709
  61. 10.1016/0092-8674(85)90098-4
  62. 10.1083/jcb.107.2.427
  63. 10.1016/S0021-9258(19)62353-7 / J. Biol. Chem. / Keratin filaments in cultured human epidermal cells. (1978)
  64. Sun, T.-T., R. Eichner, A. Schermer, D. Cooper, W.G. Nelson, and R.A. Weiss. 1984. Classification, expression, and possible mechanisms of evolution of mammalian epithelial keratins: a unifying model. In The cancer cell. Vol. 1. The transformed phenotype. (ed. A. Levine, W. Topp, G. van de Woude, and J.D. Watson), pp. 169–176. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  65. 10.1073/pnas.77.9.5201
  66. {'key': '2021111319494807000_3.1.1.66', 'first-page': '4683', 'article-title': 'Electrophoretic transfer of proteins from polyacrylamide gels onto nitrocellulose sheets: Procedure and some applications.', 'volume': '81', 'year': '1979', 'journal-title': 'Proc. Natl. Acad. Sci.'} / Proc. Natl. Acad. Sci. / Electrophoretic transfer of proteins from polyacrylamide gels onto nitrocellulose sheets: Procedure and some applications. (1979)
  67. 10.1083/jcb.103.5.1945
  68. 10.1083/jcb.99.6.2211
  69. 10.1083/jcb.98.4.1397
  70. 10.1016/0012-1606(65)90007-2
  71. 10.1016/0092-8674(82)90324-5
Dates
Type When
Created 18 years, 2 months ago (June 5, 2007, 5:15 p.m.)
Deposited 3 years, 9 months ago (Nov. 13, 2021, 10:50 p.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued 36 years, 7 months ago (Jan. 1, 1989)
Published 36 years, 7 months ago (Jan. 1, 1989)
Published Online 36 years, 7 months ago (Jan. 1, 1989)
Published Print 36 years, 7 months ago (Jan. 1, 1989)
Funders 0

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@article{Kopan_1989, title={A new look into an old problem: keratins as tools to investigate determination, morphogenesis, and differentiation in skin.}, volume={3}, ISSN={1549-5477}, url={http://dx.doi.org/10.1101/gad.3.1.1}, DOI={10.1101/gad.3.1.1}, number={1}, journal={Genes & Development}, publisher={Cold Spring Harbor Laboratory}, author={Kopan, R and Fuchs, E}, year={1989}, month=jan, pages={1–15} }